{"id":{"repo_id":"laurentian","oai_identifier":"oai:laurentian.scholaris.ca:10219/2399"},"canonical_url":"https://search.dev.ndltd.org/etd/laurentian/oai:laurentian.scholaris.ca:10219/2399","repository":{"repo_id":"laurentian","name":"Laurentian University","base_url":"https://laurentian.scholaris.ca/server/oai/request"},"display":{"title":"Intramuscular immunization of mice with KatA (catalase) generates functional antibodies against Campylobacter jejuni invasion of gut epithelial cells in vitro","abstract":"The high incidence of Campylobacter jejuni-associated diarrhea, increase in the frequency of drug resistance in clinical isolates, and the recent association of C. jejuni infections and Guillain-Barré syndrome has heightened the need to develop effective anti-Campylobacter vaccines. Due to the risk of auto-immunity, vaccination using inactivated whole-cells or attenuated cells cannot be considered. Subunit vaccines however are a viable alternative and have been shown to be effective against similar pathogens. The main goal of this research is to test efficacy of the catalase A (KatA) protein as vaccine candidate against this pathogen. It was determined that KatA co-fractionates with both the soluble and insoluble cell fractions and a balanced IgG1/IgG2a response could be produced against it following immunization in mice. It was demonstrated that these antibodies can mediate complement-specific bactericidal activity and reduced adhesion and invasion of human epithelial (Caco-2) cells by C. jejuni using established in vitro assays.","abstract_html":"The high incidence of Campylobacter jejuni-associated diarrhea, increase in the frequency of drug resistance in clinical isolates, and the recent association of C. jejuni infections and Guillain-Barré syndrome has heightened the need to develop effective anti-Campylobacter vaccines. Due to the risk of auto-immunity, vaccination using inactivated whole-cells or attenuated cells cannot be considered. Subunit vaccines however are a viable alternative and have been shown to be effective against similar pathogens. The main goal of this research is to test efficacy of the catalase A (KatA) protein as vaccine candidate against this pathogen. It was determined that KatA co-fractionates with both the soluble and insoluble cell fractions and a balanced IgG1/IgG2a response could be produced against it following immunization in mice. It was demonstrated that these antibodies can mediate complement-specific bactericidal activity and reduced adhesion and invasion of human epithelial (Caco-2) cells by C. jejuni using established in vitro assays.","abstract_has_math":false,"creators":["Rickaby, Barbara Ashley"],"institution":"Laurentian University of Sudbury","degree_name":"Master's Theses","degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-05-27","date_published":"2015-05-27","updated_at":"2026-08-21T16:45:57Z","subjects":["Campylobacter jejuni","catalase","functional antibodies","KatA","vaccine,"],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://laurentian.scholaris.ca/handle/10219/2399","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"source_record":{"url":"https://laurentian.scholaris.ca/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Alaurentian.scholaris.ca%3A10219%2F2399","prefix":"dim"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Rickaby, Barbara Ashley"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-05-27T15:44:12Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-05-27T15:44:12Z"]},{"key":"dc:date.issued","label":"Date","values":["2015-05-27"]},{"key":"dc:publisher","label":"Institution","values":["Laurentian University of Sudbury"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master's Theses"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Laurentian University of Sudbury"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Campylobacter jejuni","catalase","functional antibodies","KatA","vaccine,"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://laurentian.scholaris.ca/handle/10219/2399"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The high incidence of Campylobacter jejuni-associated diarrhea, increase in the frequency of drug resistance in clinical isolates, and the recent association of C. jejuni infections and Guillain-Barré syndrome has heightened the need to develop effective anti-Campylobacter vaccines. Due to the risk of auto-immunity, vaccination using inactivated whole-cells or attenuated cells cannot be considered. Subunit vaccines however are a viable alternative and have been shown to be effective against similar pathogens. The main goal of this research is to test efficacy of the catalase A (KatA) protein as vaccine candidate against this pathogen. It was determined that KatA co-fractionates with both the soluble and insoluble cell fractions and a balanced IgG1/IgG2a response could be produced against it following immunization in mice. It was demonstrated that these antibodies can mediate complement-specific bactericidal activity and reduced adhesion and invasion of human epithelial (Caco-2) cells by C. jejuni using established in vitro assays."]},{"key":"dc:title","label":"Title","values":["Intramuscular immunization of mice with KatA (catalase) generates functional antibodies against Campylobacter jejuni invasion of gut epithelial cells in vitro"]}]}],"canonical_facts":{"dc:creator":["Rickaby, Barbara Ashley"],"dc:date.accessioned":["2015-05-27T15:44:12Z"],"dc:date.available":["2015-05-27T15:44:12Z"],"dc:date.issued":["2015-05-27"],"dc:description.abstract":["The high incidence of Campylobacter jejuni-associated diarrhea, increase in the frequency of drug resistance in clinical isolates, and the recent association of C. jejuni infections and Guillain-Barré syndrome has heightened the need to develop effective anti-Campylobacter vaccines. Due to the risk of auto-immunity, vaccination using inactivated whole-cells or attenuated cells cannot be considered. Subunit vaccines however are a viable alternative and have been shown to be effective against similar pathogens. The main goal of this research is to test efficacy of the catalase A (KatA) protein as vaccine candidate against this pathogen. It was determined that KatA co-fractionates with both the soluble and insoluble cell fractions and a balanced IgG1/IgG2a response could be produced against it following immunization in mice. It was demonstrated that these antibodies can mediate complement-specific bactericidal activity and reduced adhesion and invasion of human epithelial (Caco-2) cells by C. jejuni using established in vitro assays."],"dc:identifier.uri":["https://laurentian.scholaris.ca/handle/10219/2399"],"dc:language.iso":["en_US"],"dc:publisher":["Laurentian University of Sudbury"],"dc:subject":["Campylobacter jejuni","catalase","functional antibodies","KatA","vaccine,"],"dc:title":["Intramuscular immunization of mice with KatA (catalase) generates functional antibodies against Campylobacter jejuni invasion of gut epithelial cells in vitro"],"dc:type":["Thesis"],"thesis:degree_name":["Master's Theses"],"thesis:institution_name":["Laurentian University of Sudbury"]},"updated_at":"2026-08-21T16:45:57Z"}