{"id":{"repo_id":"lancaster","oai_identifier":"oai:eprints.lancs.ac.uk:82398"},"canonical_url":"https://search.dev.ndltd.org/etd/lancaster/oai:eprints.lancs.ac.uk:82398","repository":{"repo_id":"lancaster","name":"Lancaster University","base_url":"https://eprints.lancs.ac.uk/cgi/oai2"},"display":{"title":"Biospectroscopy investigations Into cervical cytology","abstract":"Local treatment for cervical intra-epithelial neoplasia (CIN) involves the removal of the affected part of the tissue and is >95% effective in preventing re-invasive disease. However, removal of part of the cervix is linked to significant adverse sequelae, including preterm birth; with cone depth and radicality of treatment correlating to the frequency and severity of adverse events. Since pre-treatment cervix length vary amongst women, the percentage of cervix excised may correlate more accurately to risk than absolute dimensions. Attenuated total reflectance, Fourier-transform infra-red (ATR-FTIR) spectroscopy detected that treatment for CIN significantly alters the biochemical fingerprint in the cervix, compared with women who have not had treatment; this is due to the excision of cervical tissue rather than a disease controlling effect. However, the spectra did not correlate to the cone depth or proportion of cervical length excised. Post-hoc analyses of patient characteristics found that spectral absorbance was different for treated women according to whether they were current/non-smokers; nulliparous/parous; by luteal/follicular phase; and by combined oral contraceptive pill use; these patient characteristics are likely to have affected the excisional outcomes. As traditional IR techniques are limited by the effect of diffraction of ~3 μm to 30 μm, we assessed the potential of scanning near-field optical microscopy in combination with an IR free electron laser (SNOM-IR-FEL), in determining the biophysical properties of abnormal cervical cells. SNOM-IR-FEL is able to distinguish between normal and various grades of cervical abnormalities at designated wavelengths associated with DNA, amides I and II and lipids, at spatial resolutions below the diffraction limit (≥0.2 μm).","abstract_html":"Local treatment for cervical intra-epithelial neoplasia (CIN) involves the removal of the affected part of the tissue and is >95% effective in preventing re-invasive disease. However, removal of part of the cervix is linked to significant adverse sequelae, including preterm birth; with cone depth and radicality of treatment correlating to the frequency and severity of adverse events. Since pre-treatment cervix length vary amongst women, the percentage of cervix excised may correlate more accurately to risk than absolute dimensions. Attenuated total reflectance, Fourier-transform infra-red (ATR-FTIR) spectroscopy detected that treatment for CIN significantly alters the biochemical fingerprint in the cervix, compared with women who have not had treatment; this is due to the excision of cervical tissue rather than a disease controlling effect. However, the spectra did not correlate to the cone depth or proportion of cervical length excised. Post-hoc analyses of patient characteristics found that spectral absorbance was different for treated women according to whether they were current/non-smokers; nulliparous/parous; by luteal/follicular phase; and by combined oral contraceptive pill use; these patient characteristics are likely to have affected the excisional outcomes. As traditional IR techniques are limited by the effect of diffraction of ~3 μm to 30 μm, we assessed the potential of scanning near-field optical microscopy in combination with an IR free electron laser (SNOM-IR-FEL), in determining the biophysical properties of abnormal cervical cells. SNOM-IR-FEL is able to distinguish between normal and various grades of cervical abnormalities at designated wavelengths associated with DNA, amides I and II and lipids, at spatial resolutions below the diffraction limit (≥0.2 μm).","abstract_has_math":false,"creators":["Halliwell, Diane Elizabeth","Martin, Frank"],"institution":"Lancaster University","degree_name":"masters","degree_level":"masters","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016","date_published":"2016","updated_at":"2026-07-24T02:48:30Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Halliwell, Diane Elizabeth","Martin, Frank"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016"]},{"key":"dc:date.issued","label":"Date","values":["2016"]},{"key":"dc:publisher.commercial","label":"Dc Publisher Commercial","values":["Lancaster University"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Lancaster Environment Centre"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Lancaster University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://eprints.lancs.ac.uk/id/eprint/82398/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["masters"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://eprints.lancs.ac.uk/id/eprint/82398/1/2016HalliwellDEMRes.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Local treatment for cervical intra-epithelial neoplasia (CIN) involves the removal of the affected part of the tissue and is >95% effective in preventing re-invasive disease. However, removal of part of the cervix is linked to significant adverse sequelae, including preterm birth; with cone depth and radicality of treatment correlating to the frequency and severity of adverse events. Since pre-treatment cervix length vary amongst women, the percentage of cervix excised may correlate more accurately to risk than absolute dimensions. Attenuated total reflectance, Fourier-transform infra-red (ATR-FTIR) spectroscopy detected that treatment for CIN significantly alters the biochemical fingerprint in the cervix, compared with women who have not had treatment; this is due to the excision of cervical tissue rather than a disease controlling effect. However, the spectra did not correlate to the cone depth or proportion of cervical length excised. Post-hoc analyses of patient characteristics found that spectral absorbance was different for treated women according to whether they were current/non-smokers; nulliparous/parous; by luteal/follicular phase; and by combined oral contraceptive pill use; these patient characteristics are likely to have affected the excisional outcomes. As traditional IR techniques are limited by the effect of diffraction of ~3 μm to 30 μm, we assessed the potential of scanning near-field optical microscopy in combination with an IR free electron laser (SNOM-IR-FEL), in determining the biophysical properties of abnormal cervical cells. SNOM-IR-FEL is able to distinguish between normal and various grades of cervical abnormalities at designated wavelengths associated with DNA, amides I and II and lipids, at spatial resolutions below the diffraction limit (≥0.2 μm)."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Biospectroscopy investigations Into cervical cytology"]}]}],"canonical_facts":{"dc:creator":["Halliwell, Diane Elizabeth","Martin, Frank"],"dc:date":["2016"],"dc:date.issued":["2016"],"dc:description.abstract":["Local treatment for cervical intra-epithelial neoplasia (CIN) involves the removal of the affected part of the tissue and is >95% effective in preventing re-invasive disease. However, removal of part of the cervix is linked to significant adverse sequelae, including preterm birth; with cone depth and radicality of treatment correlating to the frequency and severity of adverse events. Since pre-treatment cervix length vary amongst women, the percentage of cervix excised may correlate more accurately to risk than absolute dimensions. Attenuated total reflectance, Fourier-transform infra-red (ATR-FTIR) spectroscopy detected that treatment for CIN significantly alters the biochemical fingerprint in the cervix, compared with women who have not had treatment; this is due to the excision of cervical tissue rather than a disease controlling effect. However, the spectra did not correlate to the cone depth or proportion of cervical length excised. Post-hoc analyses of patient characteristics found that spectral absorbance was different for treated women according to whether they were current/non-smokers; nulliparous/parous; by luteal/follicular phase; and by combined oral contraceptive pill use; these patient characteristics are likely to have affected the excisional outcomes. As traditional IR techniques are limited by the effect of diffraction of ~3 μm to 30 μm, we assessed the potential of scanning near-field optical microscopy in combination with an IR free electron laser (SNOM-IR-FEL), in determining the biophysical properties of abnormal cervical cells. SNOM-IR-FEL is able to distinguish between normal and various grades of cervical abnormalities at designated wavelengths associated with DNA, amides I and II and lipids, at spatial resolutions below the diffraction limit (≥0.2 μm)."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://eprints.lancs.ac.uk/id/eprint/82398/1/2016HalliwellDEMRes.pdf"],"dc:publisher.commercial":["Lancaster University"],"dc:publisher.department":["Lancaster Environment Centre"],"dc:publisher.institution":["Lancaster University"],"dc:relation.isreferencedby":["https://eprints.lancs.ac.uk/id/eprint/82398/"],"dc:title":["Biospectroscopy investigations Into cervical cytology"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["masters"],"dc:type.qualificationname":["masters"]},"updated_at":"2026-07-24T02:48:30Z"}