Community Dynamics of Freshwater Picocyanobacteria and Development and Application of HIP 1 PCR (Cyanobacterial Typing Technique).
type: CreativeWork
description: The main aim of this study was to further our understanding of the diversity of a freshwater picocyanobacterial community. The HIP 1 PCR typing technique was developed and applied to 506 isolates of picocyanobacteria from a field study on Esthwaite Water in 2000. This study has demonstrated that genetic diversity existed within the picocyanobacteria characterised morphologically by rod shaped cells and high phycocyanin pigment content. Picocyanobacteria of this morphology represented a significant proportion of the picocyanobacterial community within the lake in 2000, which was typical of summer conditions for that lake. Twenty-one HIP 1 types were defined; ninety isolates were not assigned to a type as they generated HIP 1 PCR products which were insufficiently similar to less than five other isolates. The diversity of HIP 1 PCR products generated from isolates within the types ranged from homogeneous complex patterns to similarity of one key PCR product and heterogeneity of minor products. It is likely that on further isolation and testing of isolates within the more heterogeneous types further splitting of these types will be appropriate. The HIP 1 types were each re-isolated on at least two sampling occasions (one month apart). Some types were isolated from sites spanning the sampling period demonstrating that there was some stability within the HIP 1 types within the picocyanobacterial community within Esthwaite Water over the summer of 2000. Some types appeared to be isolated more successfully at different times of the sampling period suggesting a temporal shift/changes of HIP 1 types in the picocyanobacterial community between June and October in Esthwaite Water. It was possible to demonstrate that three HIP 1 types were isolated at statistically different success rates from some spatial or temporal locations. It was concluded that picocyanobacteria are considerably more diverse than indicated by gross morphology. Picocyanobacteria of different genotypes may …
abstract: The main aim of this study was to further our understanding of the diversity of a freshwater picocyanobacterial community. The HIP 1 PCR typing technique was developed and applied to 506 isolates of picocyanobacteria from a field study on Esthwaite Water in 2000. This study has demonstrated that genetic diversity existed within the picocyanobacteria characterised morphologically by rod shaped cells and high phycocyanin pigment content. Picocyanobacteria of this morphology represented a significant proportion of the picocyanobacterial community within the lake in 2000, which was typical of summer conditions for that lake. Twenty-one HIP 1 types were defined; ninety isolates were not assigned to a type as they generated HIP 1 PCR products which were insufficiently similar to less than five other isolates. The diversity of HIP 1 PCR products generated from isolates within the types ranged from homogeneous complex patterns to similarity of one key PCR product and heterogeneity of minor products. It is likely that on further isolation and testing of isolates within the more heterogeneous types further splitting of these types will be appropriate. The HIP 1 types were each re-isolated on at least two sampling occasions (one month apart). Some types were isolated from sites spanning the sampling period demonstrating that there was some stability within the HIP 1 types within the picocyanobacterial community within Esthwaite Water over the summer of 2000. Some types appeared to be isolated more successfully at different times of the sampling period suggesting a temporal shift/changes of HIP 1 types in the picocyanobacterial community between June and October in Esthwaite Water. It was possible to demonstrate that three HIP 1 types were isolated at statistically different success rates from some spatial or temporal locations. It was concluded that picocyanobacteria are considerably more diverse than indicated by gross morphology. Picocyanobacteria of different genotypes may …
author: Harper, Katie Johanna
date published: 2005
date modified: 2026-07-24T02:48:52Z
provider: Lancaster University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Lancaster University