{"id":{"repo_id":"ku","oai_identifier":"oai:kuscholarworks.ku.edu:1808/9725"},"canonical_url":"https://search.dev.ndltd.org/etd/ku/oai:kuscholarworks.ku.edu:1808/9725","repository":{"repo_id":"ku","name":"University of Kansas","base_url":"https://kuscholarworks.ku.edu/server/oai/request"},"display":{"title":"The interaction of ELKS with Ca2+ channel β subunits may contribute to active zone organization","abstract":"The cytoskeletal matrix of the active zone (CAZ) and synaptic voltage-dependent calcium channels (VDCCs) are both required for the organization and regulation of synaptic vesicle release. Here we report a novel interaction between the CAZ protein family ELKS and VDCC β subunits. We found that two isoforms of ELKS (ELKS2 and ELKS1b) and two isoforms of VDCC β subunits (β1b and β4) co-immunoprecipitated. Using fluorescent immunohistochemistry, we observed colocalization between ELKS1b and VDCC β4 at synapses in the molecular layer of the cerebellum, suggesting a synaptic role for the interaction of these two proteins. Analysis of a P/Q-type VDCC knockout mouse (Cacna1a-/-) revealed that the localization of the VDCC β4 subunit to the molecular layer was disrupted, although ELKS1b protein localization was not affected. The results demonstrate that two isoforms of the ELKS family and two VDCC β subunits interact and suggest that their interaction may play a role in the organization of the active zone.","abstract_html":"The cytoskeletal matrix of the active zone (CAZ) and synaptic voltage-dependent calcium channels (VDCCs) are both required for the organization and regulation of synaptic vesicle release. Here we report a novel interaction between the CAZ protein family ELKS and VDCC β subunits. We found that two isoforms of ELKS (ELKS2 and ELKS1b) and two isoforms of VDCC β subunits (β1b and β4) co-immunoprecipitated. Using fluorescent immunohistochemistry, we observed colocalization between ELKS1b and VDCC β4 at synapses in the molecular layer of the cerebellum, suggesting a synaptic role for the interaction of these two proteins. Analysis of a P/Q-type VDCC knockout mouse (Cacna1a-/-) revealed that the localization of the VDCC β4 subunit to the molecular layer was disrupted, although ELKS1b protein localization was not affected. The results demonstrate that two isoforms of the ELKS family and two VDCC β subunits interact and suggest that their interaction may play a role in the organization of the active zone.","abstract_has_math":false,"creators":["Billings, Sara Elizabeth"],"institution":"University of Kansas","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Nishimune, Hiroshi"],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-12-31","date_published":"2011-12-31","updated_at":"2026-07-24T02:44:44Z","subjects":["Neurosciences","Cell biology","Biology","Active zone","Cast","Cytomatrix of the active zone","Elks","Synapse","Voltage-dependent calcium channel"],"languages":["en_US"],"rights":["This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["http://dissertations.umi.com/ku:11840"],"render_values":[{"text":"http://dissertations.umi.com/ku:11840","href":"http://dissertations.umi.com/ku:11840","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/1808/9725","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Nishimune, Hiroshi"]},{"key":"dc:creator","label":"Author","values":["Billings, Sara Elizabeth"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2012-06-03T14:11:10Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2012-06-03T14:11:10Z"]},{"key":"dc:date.issued","label":"Date","values":["2011-12-31"]},{"key":"dc:publisher","label":"Institution","values":["University of Kansas"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Neurosciences","Cell biology","Biology","Active zone","Cast","Cytomatrix of the active zone","Elks","Synapse","Voltage-dependent calcium channel"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["http://dissertations.umi.com/ku:11840"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1808/9725"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The cytoskeletal matrix of the active zone (CAZ) and synaptic voltage-dependent calcium channels (VDCCs) are both required for the organization and regulation of synaptic vesicle release. Here we report a novel interaction between the CAZ protein family ELKS and VDCC β subunits. We found that two isoforms of ELKS (ELKS2 and ELKS1b) and two isoforms of VDCC β subunits (β1b and β4) co-immunoprecipitated. Using fluorescent immunohistochemistry, we observed colocalization between ELKS1b and VDCC β4 at synapses in the molecular layer of the cerebellum, suggesting a synaptic role for the interaction of these two proteins. Analysis of a P/Q-type VDCC knockout mouse (Cacna1a-/-) revealed that the localization of the VDCC β4 subunit to the molecular layer was disrupted, although ELKS1b protein localization was not affected. The results demonstrate that two isoforms of the ELKS family and two VDCC β subunits interact and suggest that their interaction may play a role in the organization of the active zone."]},{"key":"dc:title","label":"Title","values":["The interaction of ELKS with Ca2+ channel β subunits may contribute to active zone organization"]}]}],"canonical_facts":{"dc:contributor.advisor":["Nishimune, Hiroshi"],"dc:creator":["Billings, Sara Elizabeth"],"dc:date.accessioned":["2012-06-03T14:11:10Z"],"dc:date.available":["2012-06-03T14:11:10Z"],"dc:date.issued":["2011-12-31"],"dc:description.abstract":["The cytoskeletal matrix of the active zone (CAZ) and synaptic voltage-dependent calcium channels (VDCCs) are both required for the organization and regulation of synaptic vesicle release. Here we report a novel interaction between the CAZ protein family ELKS and VDCC β subunits. We found that two isoforms of ELKS (ELKS2 and ELKS1b) and two isoforms of VDCC β subunits (β1b and β4) co-immunoprecipitated. Using fluorescent immunohistochemistry, we observed colocalization between ELKS1b and VDCC β4 at synapses in the molecular layer of the cerebellum, suggesting a synaptic role for the interaction of these two proteins. Analysis of a P/Q-type VDCC knockout mouse (Cacna1a-/-) revealed that the localization of the VDCC β4 subunit to the molecular layer was disrupted, although ELKS1b protein localization was not affected. The results demonstrate that two isoforms of the ELKS family and two VDCC β subunits interact and suggest that their interaction may play a role in the organization of the active zone."],"dc:identifier.other":["http://dissertations.umi.com/ku:11840"],"dc:identifier.uri":["http://hdl.handle.net/1808/9725"],"dc:language.iso":["en_US"],"dc:publisher":["University of Kansas"],"dc:rights":["This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author."],"dc:subject":["Neurosciences","Cell biology","Biology","Active zone","Cast","Cytomatrix of the active zone","Elks","Synapse","Voltage-dependent calcium channel"],"dc:title":["The interaction of ELKS with Ca2+ channel β subunits may contribute to active zone organization"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T02:44:44Z"}