{"id":{"repo_id":"ku","oai_identifier":"oai:kuscholarworks.ku.edu:1808/7747"},"canonical_url":"https://search.dev.ndltd.org/etd/ku/oai:kuscholarworks.ku.edu:1808/7747","repository":{"repo_id":"ku","name":"University of Kansas","base_url":"https://kuscholarworks.ku.edu/server/oai/request"},"display":{"title":"RNA Helicase 1 interacts with an ABCRNAi Transporter: Genetic Interactions with haf-6","abstract":"The C. elegans rha-1 gene encodes a conserved helicase with ATP-dependent DEAD/H-box and double-stranded RNA binding domains. rha-1 is orthologous to the Drosophila maleless gene(MLE), an essential component of the dosage compensation machinery that leads to a two-fold increase in transcription of genes located on the single X chromosome of males in comparison to the transcription rate of homologous genes located on an X chromosome in females. The human ortholog, RNA helicase A (RHA), unwinds double-stranded DNA and RNA in a 3' to 5' direction. RHA is a component of several distinct protein complexes, including the RNA-induced silencing complex (RISC), the coding region determinant (CRD)-mediated complex, mRNP granules, and also associates with BRCA1, CREBbp or SMN1 and the RNA polymerase II complex, phosphorylated histones (H2AFX). RHA affects a number of different biological activities, including CRD-mediated mRNA stabilization, RNA splicing, translation, and transcription. It also has been discovered that regulation of RHA is disrupted in many types of cancers. The C. elegans rha-1 gene is expressed in the gonad where it localizes to the nucleus and the cytoplasm and is required for proper development. Defects in rha-1 lead to germline defects, aberrant expression of genes residing in repetitive transgene arrays, and defects in RNAi. ABC transporter proteins have also been implicated to play a role in RNAi in C. elegans. Ten out of the sixty-one ABC transporters are required for efficient RNAi in the germ line; these transporters are called ABCRNAi transporters. All of the ABCRNAi transporter mutants interact genetically with rde-2 (a novel protein) and mut-7 (a protein with homology to RnaseD). One of these ABCRNAi transporter genes is haf-6. The works presented here show that rha-1 mutants also interact genetically with haf-6, with rde-2, and with mut-7 with respect to RNAi defects. By contrast, mutations in rha-1 suppress the transposon mobilization phenotypes observed in haf-6 mutants. It is also observed that an unknown mutation is genetically interacting with rha-1 and haf-6. We are currently investigating roles for rha-1 in proper accumulation of haf-6 and other ABCRNAi transporter mRNAs in an effort to understand the interrelated functions of these proteins in RNAi.","abstract_html":"The C. elegans rha-1 gene encodes a conserved helicase with ATP-dependent DEAD/H-box and double-stranded RNA binding domains. rha-1 is orthologous to the Drosophila maleless gene(MLE), an essential component of the dosage compensation machinery that leads to a two-fold increase in transcription of genes located on the single X chromosome of males in comparison to the transcription rate of homologous genes located on an X chromosome in females. The human ortholog, RNA helicase A (RHA), unwinds double-stranded DNA and RNA in a 3' to 5' direction. RHA is a component of several distinct protein complexes, including the RNA-induced silencing complex (RISC), the coding region determinant (CRD)-mediated complex, mRNP granules, and also associates with BRCA1, CREBbp or SMN1 and the RNA polymerase II complex, phosphorylated histones (H2AFX). RHA affects a number of different biological activities, including CRD-mediated mRNA stabilization, RNA splicing, translation, and transcription. It also has been discovered that regulation of RHA is disrupted in many types of cancers. The C. elegans rha-1 gene is expressed in the gonad where it localizes to the nucleus and the cytoplasm and is required for proper development. Defects in rha-1 lead to germline defects, aberrant expression of genes residing in repetitive transgene arrays, and defects in RNAi. ABC transporter proteins have also been implicated to play a role in RNAi in C. elegans. Ten out of the sixty-one ABC transporters are required for efficient RNAi in the germ line; these transporters are called ABCRNAi transporters. All of the ABCRNAi transporter mutants interact genetically with rde-2 (a novel protein) and mut-7 (a protein with homology to RnaseD). One of these ABCRNAi transporter genes is haf-6. The works presented here show that rha-1 mutants also interact genetically with haf-6, with rde-2, and with mut-7 with respect to RNAi defects. By contrast, mutations in rha-1 suppress the transposon mobilization phenotypes observed in haf-6 mutants. It is also observed that an unknown mutation is genetically interacting with rha-1 and haf-6. We are currently investigating roles for rha-1 in proper accumulation of haf-6 and other ABCRNAi transporter mRNAs in an effort to understand the interrelated functions of these proteins in RNAi.","abstract_has_math":false,"creators":["Rivera, Laticia"],"institution":"University of Kansas","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Timmons, Lisa"],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-12-03","date_published":"2010-12-03","updated_at":"2026-07-24T02:46:40Z","subjects":["Genetics","Abc transporter","Haf-6","Rnai"],"languages":["en"],"rights":["This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["http://dissertations.umi.com/ku:11213"],"render_values":[{"text":"http://dissertations.umi.com/ku:11213","href":"http://dissertations.umi.com/ku:11213","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/1808/7747","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Timmons, Lisa"]},{"key":"dc:creator","label":"Author","values":["Rivera, Laticia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2011-07-04T19:22:28Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2011-07-04T19:22:28Z"]},{"key":"dc:date.issued","label":"Date","values":["2010-12-03"]},{"key":"dc:publisher","label":"Institution","values":["University of Kansas"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Genetics","Abc transporter","Haf-6","Rnai"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["http://dissertations.umi.com/ku:11213"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1808/7747"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The C. elegans rha-1 gene encodes a conserved helicase with ATP-dependent DEAD/H-box and double-stranded RNA binding domains. rha-1 is orthologous to the Drosophila maleless gene(MLE), an essential component of the dosage compensation machinery that leads to a two-fold increase in transcription of genes located on the single X chromosome of males in comparison to the transcription rate of homologous genes located on an X chromosome in females. The human ortholog, RNA helicase A (RHA), unwinds double-stranded DNA and RNA in a 3' to 5' direction. RHA is a component of several distinct protein complexes, including the RNA-induced silencing complex (RISC), the coding region determinant (CRD)-mediated complex, mRNP granules, and also associates with BRCA1, CREBbp or SMN1 and the RNA polymerase II complex, phosphorylated histones (H2AFX). RHA affects a number of different biological activities, including CRD-mediated mRNA stabilization, RNA splicing, translation, and transcription. It also has been discovered that regulation of RHA is disrupted in many types of cancers. The C. elegans rha-1 gene is expressed in the gonad where it localizes to the nucleus and the cytoplasm and is required for proper development. Defects in rha-1 lead to germline defects, aberrant expression of genes residing in repetitive transgene arrays, and defects in RNAi. ABC transporter proteins have also been implicated to play a role in RNAi in C. elegans. Ten out of the sixty-one ABC transporters are required for efficient RNAi in the germ line; these transporters are called ABCRNAi transporters. All of the ABCRNAi transporter mutants interact genetically with rde-2 (a novel protein) and mut-7 (a protein with homology to RnaseD). One of these ABCRNAi transporter genes is haf-6. The works presented here show that rha-1 mutants also interact genetically with haf-6, with rde-2, and with mut-7 with respect to RNAi defects. By contrast, mutations in rha-1 suppress the transposon mobilization phenotypes observed in haf-6 mutants. It is also observed that an unknown mutation is genetically interacting with rha-1 and haf-6. We are currently investigating roles for rha-1 in proper accumulation of haf-6 and other ABCRNAi transporter mRNAs in an effort to understand the interrelated functions of these proteins in RNAi."]},{"key":"dc:title","label":"Title","values":["RNA Helicase 1 interacts with an ABCRNAi Transporter: Genetic Interactions with haf-6"]}]}],"canonical_facts":{"dc:contributor.advisor":["Timmons, Lisa"],"dc:creator":["Rivera, Laticia"],"dc:date.accessioned":["2011-07-04T19:22:28Z"],"dc:date.available":["2011-07-04T19:22:28Z"],"dc:date.issued":["2010-12-03"],"dc:description.abstract":["The C. elegans rha-1 gene encodes a conserved helicase with ATP-dependent DEAD/H-box and double-stranded RNA binding domains. rha-1 is orthologous to the Drosophila maleless gene(MLE), an essential component of the dosage compensation machinery that leads to a two-fold increase in transcription of genes located on the single X chromosome of males in comparison to the transcription rate of homologous genes located on an X chromosome in females. The human ortholog, RNA helicase A (RHA), unwinds double-stranded DNA and RNA in a 3' to 5' direction. RHA is a component of several distinct protein complexes, including the RNA-induced silencing complex (RISC), the coding region determinant (CRD)-mediated complex, mRNP granules, and also associates with BRCA1, CREBbp or SMN1 and the RNA polymerase II complex, phosphorylated histones (H2AFX). RHA affects a number of different biological activities, including CRD-mediated mRNA stabilization, RNA splicing, translation, and transcription. It also has been discovered that regulation of RHA is disrupted in many types of cancers. The C. elegans rha-1 gene is expressed in the gonad where it localizes to the nucleus and the cytoplasm and is required for proper development. Defects in rha-1 lead to germline defects, aberrant expression of genes residing in repetitive transgene arrays, and defects in RNAi. ABC transporter proteins have also been implicated to play a role in RNAi in C. elegans. Ten out of the sixty-one ABC transporters are required for efficient RNAi in the germ line; these transporters are called ABCRNAi transporters. All of the ABCRNAi transporter mutants interact genetically with rde-2 (a novel protein) and mut-7 (a protein with homology to RnaseD). One of these ABCRNAi transporter genes is haf-6. The works presented here show that rha-1 mutants also interact genetically with haf-6, with rde-2, and with mut-7 with respect to RNAi defects. By contrast, mutations in rha-1 suppress the transposon mobilization phenotypes observed in haf-6 mutants. It is also observed that an unknown mutation is genetically interacting with rha-1 and haf-6. We are currently investigating roles for rha-1 in proper accumulation of haf-6 and other ABCRNAi transporter mRNAs in an effort to understand the interrelated functions of these proteins in RNAi."],"dc:identifier.other":["http://dissertations.umi.com/ku:11213"],"dc:identifier.uri":["http://hdl.handle.net/1808/7747"],"dc:language.iso":["en"],"dc:publisher":["University of Kansas"],"dc:rights":["This item is protected by copyright and unless otherwise specified the copyright of this thesis/dissertation is held by the author."],"dc:subject":["Genetics","Abc transporter","Haf-6","Rnai"],"dc:title":["RNA Helicase 1 interacts with an ABCRNAi Transporter: Genetic Interactions with haf-6"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T02:46:40Z"}