Modulating Cariogenic Interfaces Through Machine Learning-Guided Peptide Design
type: CreativeWork
description: In 2025, dental caries remains one of the most prevalent chronic diseases worldwide. Cariostatic agents such as silver diamine fluoride (SDF) have been receiving steadily increasing interest due to their efficiency, minimal intervention requirements, short application time, and low cost. SDF is an easily applied topical solution that can be used to arrest carious decay. It first gained interest in pediatric dentistry and is now emerging as a nonsurgical treatment option for treating caries in elderly and adult populations with special needs due to their pre-existing medical conditions. While SDF treatments offer speed and ease in caries management, making it readily accessible for a wider range of patients, its utilization remains limited. Several factors affect this outcome, including long-term efficacy, impact on future restorations due to modified tissue interfaces, and black staining. Upon application, SDF undergoes a series of chemical reactions on dental tissues that lead to tissue restoration with these concerning factors, including the permanent black staining of carious lesions, i.e., enamel and dentin. Previously, our group investigated a peptide-enabled approach for biomimetic reconstruction of SDF-treated dental tissues to mitigate the undesired effects. A silver binding peptide was explored to provide an anchoring ability on SDF-treated tissues with an additional remineralization function. This thesis further explores the design of peptides capable of targeting silver compounds formed after SDF treatments while minimizing interactions with hydroxyapatite through a generative machine-learning approach with augmentation for small data sets. Silver-binding peptide sequences are selected through a process that includes tailored physiochemical analysis and in silico assessments to discover candidates with properties that target caries-related environments and functions. Additionally, candidates underwent structural mapping along a pH spectrum designed to mim…
abstract: In 2025, dental caries remains one of the most prevalent chronic diseases worldwide. Cariostatic agents such as silver diamine fluoride (SDF) have been receiving steadily increasing interest due to their efficiency, minimal intervention requirements, short application time, and low cost. SDF is an easily applied topical solution that can be used to arrest carious decay. It first gained interest in pediatric dentistry and is now emerging as a nonsurgical treatment option for treating caries in elderly and adult populations with special needs due to their pre-existing medical conditions. While SDF treatments offer speed and ease in caries management, making it readily accessible for a wider range of patients, its utilization remains limited. Several factors affect this outcome, including long-term efficacy, impact on future restorations due to modified tissue interfaces, and black staining. Upon application, SDF undergoes a series of chemical reactions on dental tissues that lead to tissue restoration with these concerning factors, including the permanent black staining of carious lesions, i.e., enamel and dentin. Previously, our group investigated a peptide-enabled approach for biomimetic reconstruction of SDF-treated dental tissues to mitigate the undesired effects. A silver binding peptide was explored to provide an anchoring ability on SDF-treated tissues with an additional remineralization function. This thesis further explores the design of peptides capable of targeting silver compounds formed after SDF treatments while minimizing interactions with hydroxyapatite through a generative machine-learning approach with augmentation for small data sets. Silver-binding peptide sequences are selected through a process that includes tailored physiochemical analysis and in silico assessments to discover candidates with properties that target caries-related environments and functions. Additionally, candidates underwent structural mapping along a pH spectrum designed to mim…
author: Chu, Kalea
language: en
keywords: Bioengineering, Biomedical engineering, Nanotechnology, biomimetic, dental caries, machine learning, oral health, peptide
date published: 2025-01-01
date modified: 2026-07-24T02:46:29Z
provider: University of Kansas
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Kansas

PropertyValue
property ID: Handle
value: https://hdl.handle.net/1808/37957