{"id":{"repo_id":"ku","oai_identifier":"oai:kuscholarworks.ku.edu:1808/26893"},"canonical_url":"https://search.dev.ndltd.org/etd/ku/oai:kuscholarworks.ku.edu:1808/26893","repository":{"repo_id":"ku","name":"University of Kansas","base_url":"https://kuscholarworks.ku.edu/server/oai/request"},"display":{"title":"Drosophila CPEB, Orb2, a Putative Biochemical Engram of Long-term Memory","abstract":"How a transient experience creates an enduring yet dynamic memory remains a fundamental unresolved issue in studies of memory. Experience-dependent aggregation of the RNA-binding protein CPEB/Orb2 is one of the candidate mechanisms of memory maintenance. Here, using tools that allow rapid and reversible inactivation of Orb2 protein I find that Orb2 activity is required for encoding and recall of memory. Blocking the Orb2 oligomerization process by interfering with the protein phosphorylation pathway or expressing an anti-amyloidogenic peptide impairs long-term memory. Facilitating Orb2 aggregation by a DNA-J family chaperone, JJJ2, enhances the animal’s capacity to form long-term memory. Finally, I have developed tools to visualize training-dependent aggregation of Orb2. I find that aggregated Orb2 in subset of mushroom body neurons can serve as a “molecular signature” of memory and predict memory strength. My data indicate that self-sustaining aggregates of Orb2 may serve as a physical substrate of memory and provide a molecular basis for the perduring yet malleable nature of memory.","abstract_html":"How a transient experience creates an enduring yet dynamic memory remains a fundamental unresolved issue in studies of memory. Experience-dependent aggregation of the RNA-binding protein CPEB/Orb2 is one of the candidate mechanisms of memory maintenance. Here, using tools that allow rapid and reversible inactivation of Orb2 protein I find that Orb2 activity is required for encoding and recall of memory. Blocking the Orb2 oligomerization process by interfering with the protein phosphorylation pathway or expressing an anti-amyloidogenic peptide impairs long-term memory. Facilitating Orb2 aggregation by a DNA-J family chaperone, JJJ2, enhances the animal’s capacity to form long-term memory. Finally, I have developed tools to visualize training-dependent aggregation of Orb2. I find that aggregated Orb2 in subset of mushroom body neurons can serve as a “molecular signature” of memory and predict memory strength. My data indicate that self-sustaining aggregates of Orb2 may serve as a physical substrate of memory and provide a molecular basis for the perduring yet malleable nature of memory.","abstract_has_math":false,"creators":["Li, Liying"],"institution":"University of Kansas","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Si, Kausik","Cheney, Paul"],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-08-31","date_published":"2017-08-31","updated_at":"2026-07-24T02:45:19Z","subjects":["Neurosciences","Molecular biology","CPEB","engram","long-term memory","prion-like molecule"],"languages":["en"],"rights":["Copyright held by the author."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["http://dissertations.umi.com/ku:15506"],"render_values":[{"text":"http://dissertations.umi.com/ku:15506","href":"http://dissertations.umi.com/ku:15506","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/1808/26893","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Si, Kausik","Cheney, Paul"]},{"key":"dc:creator","label":"Author","values":["Li, Liying"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-10-22T15:47:31Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-10-22T15:47:31Z"]},{"key":"dc:date.issued","label":"Date","values":["2017-08-31"]},{"key":"dc:publisher","label":"Institution","values":["University of Kansas"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Neurosciences","Molecular biology","CPEB","engram","long-term memory","prion-like molecule"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright held by the author."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["http://dissertations.umi.com/ku:15506"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1808/26893"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["How a transient experience creates an enduring yet dynamic memory remains a fundamental unresolved issue in studies of memory. Experience-dependent aggregation of the RNA-binding protein CPEB/Orb2 is one of the candidate mechanisms of memory maintenance. Here, using tools that allow rapid and reversible inactivation of Orb2 protein I find that Orb2 activity is required for encoding and recall of memory. Blocking the Orb2 oligomerization process by interfering with the protein phosphorylation pathway or expressing an anti-amyloidogenic peptide impairs long-term memory. Facilitating Orb2 aggregation by a DNA-J family chaperone, JJJ2, enhances the animal’s capacity to form long-term memory. Finally, I have developed tools to visualize training-dependent aggregation of Orb2. I find that aggregated Orb2 in subset of mushroom body neurons can serve as a “molecular signature” of memory and predict memory strength. My data indicate that self-sustaining aggregates of Orb2 may serve as a physical substrate of memory and provide a molecular basis for the perduring yet malleable nature of memory."]},{"key":"dc:title","label":"Title","values":["Drosophila CPEB, Orb2, a Putative Biochemical Engram of Long-term Memory"]}]}],"canonical_facts":{"dc:contributor.advisor":["Si, Kausik","Cheney, Paul"],"dc:creator":["Li, Liying"],"dc:date.accessioned":["2018-10-22T15:47:31Z"],"dc:date.available":["2018-10-22T15:47:31Z"],"dc:date.issued":["2017-08-31"],"dc:description.abstract":["How a transient experience creates an enduring yet dynamic memory remains a fundamental unresolved issue in studies of memory. Experience-dependent aggregation of the RNA-binding protein CPEB/Orb2 is one of the candidate mechanisms of memory maintenance. Here, using tools that allow rapid and reversible inactivation of Orb2 protein I find that Orb2 activity is required for encoding and recall of memory. Blocking the Orb2 oligomerization process by interfering with the protein phosphorylation pathway or expressing an anti-amyloidogenic peptide impairs long-term memory. Facilitating Orb2 aggregation by a DNA-J family chaperone, JJJ2, enhances the animal’s capacity to form long-term memory. Finally, I have developed tools to visualize training-dependent aggregation of Orb2. I find that aggregated Orb2 in subset of mushroom body neurons can serve as a “molecular signature” of memory and predict memory strength. My data indicate that self-sustaining aggregates of Orb2 may serve as a physical substrate of memory and provide a molecular basis for the perduring yet malleable nature of memory."],"dc:identifier.other":["http://dissertations.umi.com/ku:15506"],"dc:identifier.uri":["http://hdl.handle.net/1808/26893"],"dc:language.iso":["en"],"dc:publisher":["University of Kansas"],"dc:rights":["Copyright held by the author."],"dc:subject":["Neurosciences","Molecular biology","CPEB","engram","long-term memory","prion-like molecule"],"dc:title":["Drosophila CPEB, Orb2, a Putative Biochemical Engram of Long-term Memory"],"dc:type":["Dissertation"]},"updated_at":"2026-07-24T02:45:19Z"}