{"id":{"repo_id":"kings","oai_identifier":"oai:kclpure.kcl.ac.uk:studenttheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04"},"canonical_url":"https://search.dev.ndltd.org/etd/kings/oai:kclpure.kcl.ac.uk:studenttheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04","repository":{"repo_id":"kings","name":"King's College London","base_url":"https://kclpure.kcl.ac.uk/ws/oai"},"display":{"title":"Defining the role of the transcription factor T-bet in the innate immune system in intestinal inflammation.","abstract":"Mice lacking the transcription factor T-bet in the innate immune system (Tbx21-/- x Rag2-/- Ulcerative Colitis, or TRUC) develop microbiota-dependent inflammatory bowel disease (IBD). The innate immune mechanisms responsible for causing disease are still to be resolved, yet potentially offer novel insights into the pathogenesis of IBD, and how T-bet might influence inflammation in the gut. <br/>In this thesis it was shown that chronic IBD in TRUC mice was dependent on interleukin-17A (IL-17A) producing IL-7R+ CD90+ innate lymphoid cells (ILCs). Depletion of ILCs or IL-17A neutralization cured disease. Cytokines responsible for driving innate IL-17A included IL-23, IL-6 and TL1A. IL-23 and IL-6 played functionally important roles in TRUC disease, since neutralisation reduced innate IL-17A production and attenuated disease. TNFα, predominantly produced by CD11chigh class II+ CD11b+ CD103- dendritic cells (DCs), synergised with IL-23 to drive IL-17A production by ILCs. <br/>Helicobacter typhlonius (HT) was identified as the key colitigenic bacterium driving TRUC disease. Four-five-four ribosomal RNA gene sequencing demonstrated that HT was consistently present in the faeces of all TRUC mice, but absent from a newly-derived colony of disease-free Tbx21-/- x Rag2-/- mice. Inoculation of pure HT cultures was sufficient to reinstate disease into this new colony of healthy mice. <br/>T-bet impacted on colitis in several ways. In the absence of T-bet, ILCs selectively produced IL-17A and were poor producers of interferon-γ. Second, T-bet deficient DCs produced excess TNFα in comparison with T-bet sufficient DCs. Finally, T-bet was a transcriptional repressor of the Il7ra locus. IL-7R was expressed by ILCs and signalling through this receptor was critical for TRUC disease. Selective IL-7R blockade attenuated disease. Together these data provide new insights into TRUC disease and demonstrate at least some of the mechanisms by which T-bet regulates the interplay between mucosal innate immune cells and the intestinal microbiota.","abstract_html":"Mice lacking the transcription factor T-bet in the innate immune system (Tbx21-/- x Rag2-/- Ulcerative Colitis, or TRUC) develop microbiota-dependent inflammatory bowel disease (IBD). The innate immune mechanisms responsible for causing disease are still to be resolved, yet potentially offer novel insights into the pathogenesis of IBD, and how T-bet might influence inflammation in the gut. &lt;br/&gt;In this thesis it was shown that chronic IBD in TRUC mice was dependent on interleukin-17A (IL-17A) producing IL-7R+ CD90+ innate lymphoid cells (ILCs). Depletion of ILCs or IL-17A neutralization cured disease. Cytokines responsible for driving innate IL-17A included IL-23, IL-6 and TL1A. IL-23 and IL-6 played functionally important roles in TRUC disease, since neutralisation reduced innate IL-17A production and attenuated disease. TNFα, predominantly produced by CD11chigh class II+ CD11b+ CD103- dendritic cells (DCs), synergised with IL-23 to drive IL-17A production by ILCs. &lt;br/&gt;Helicobacter typhlonius (HT) was identified as the key colitigenic bacterium driving TRUC disease. Four-five-four ribosomal RNA gene sequencing demonstrated that HT was consistently present in the faeces of all TRUC mice, but absent from a newly-derived colony of disease-free Tbx21-/- x Rag2-/- mice. Inoculation of pure HT cultures was sufficient to reinstate disease into this new colony of healthy mice. &lt;br/&gt;T-bet impacted on colitis in several ways. In the absence of T-bet, ILCs selectively produced IL-17A and were poor producers of interferon-γ. Second, T-bet deficient DCs produced excess TNFα in comparison with T-bet sufficient DCs. Finally, T-bet was a transcriptional repressor of the Il7ra locus. IL-7R was expressed by ILCs and signalling through this receptor was critical for TRUC disease. Selective IL-7R blockade attenuated disease. Together these data provide new insights into TRUC disease and demonstrate at least some of the mechanisms by which T-bet regulates the interplay between mucosal innate immune cells and the intestinal microbiota.","abstract_has_math":false,"creators":["Powell, Nicholas"],"institution":"King's College London","degree_name":"Doctor of Philosophy","degree_level":"Doctoral Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Lord, Graham Michael"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-2-1","date_published":"2014-2-1","updated_at":"2026-07-24T02:44:42Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04"],"render_values":[{"text":"oai:kclpure.kcl.ac.uk:studenttheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04","href":null,"code":true}]}]},"links":{"outbound_url":"https://kclpure.kcl.ac.uk/portal/en/studentTheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Lord, Graham Michael"]},{"key":"dc:creator","label":"Author","values":["Powell, Nicholas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-2-1"]},{"key":"dc:date.issued","label":"Date","values":["2014-2-1"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Experimental Immunobiology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["King's College London"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04","https://kclpure.kcl.ac.uk/portal/en/studentTheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://kclpure.kcl.ac.uk/portal/files/13519072/Studentthesis-Nicholas_Powell_2014.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Mice lacking the transcription factor T-bet in the innate immune system (Tbx21-/- x Rag2-/- Ulcerative Colitis, or TRUC) develop microbiota-dependent inflammatory bowel disease (IBD). The innate immune mechanisms responsible for causing disease are still to be resolved, yet potentially offer novel insights into the pathogenesis of IBD, and how T-bet might influence inflammation in the gut. <br/>In this thesis it was shown that chronic IBD in TRUC mice was dependent on interleukin-17A (IL-17A) producing IL-7R+ CD90+ innate lymphoid cells (ILCs). Depletion of ILCs or IL-17A neutralization cured disease. Cytokines responsible for driving innate IL-17A included IL-23, IL-6 and TL1A. IL-23 and IL-6 played functionally important roles in TRUC disease, since neutralisation reduced innate IL-17A production and attenuated disease. TNFα, predominantly produced by CD11chigh class II+ CD11b+ CD103- dendritic cells (DCs), synergised with IL-23 to drive IL-17A production by ILCs. <br/>Helicobacter typhlonius (HT) was identified as the key colitigenic bacterium driving TRUC disease. Four-five-four ribosomal RNA gene sequencing demonstrated that HT was consistently present in the faeces of all TRUC mice, but absent from a newly-derived colony of disease-free Tbx21-/- x Rag2-/- mice. Inoculation of pure HT cultures was sufficient to reinstate disease into this new colony of healthy mice. <br/>T-bet impacted on colitis in several ways. In the absence of T-bet, ILCs selectively produced IL-17A and were poor producers of interferon-γ. Second, T-bet deficient DCs produced excess TNFα in comparison with T-bet sufficient DCs. Finally, T-bet was a transcriptional repressor of the Il7ra locus. IL-7R was expressed by ILCs and signalling through this receptor was critical for TRUC disease. Selective IL-7R blockade attenuated disease. Together these data provide new insights into TRUC disease and demonstrate at least some of the mechanisms by which T-bet regulates the interplay between mucosal innate immune cells and the intestinal microbiota."]},{"key":"dc:title","label":"Title","values":["Defining the role of the transcription factor T-bet in the innate immune system in intestinal inflammation."]}]}],"canonical_facts":{"dc:contributor.advisor":["Lord, Graham Michael"],"dc:creator":["Powell, Nicholas"],"dc:date":["2014-2-1"],"dc:date.issued":["2014-2-1"],"dc:description.abstract":["Mice lacking the transcription factor T-bet in the innate immune system (Tbx21-/- x Rag2-/- Ulcerative Colitis, or TRUC) develop microbiota-dependent inflammatory bowel disease (IBD). The innate immune mechanisms responsible for causing disease are still to be resolved, yet potentially offer novel insights into the pathogenesis of IBD, and how T-bet might influence inflammation in the gut. <br/>In this thesis it was shown that chronic IBD in TRUC mice was dependent on interleukin-17A (IL-17A) producing IL-7R+ CD90+ innate lymphoid cells (ILCs). Depletion of ILCs or IL-17A neutralization cured disease. Cytokines responsible for driving innate IL-17A included IL-23, IL-6 and TL1A. IL-23 and IL-6 played functionally important roles in TRUC disease, since neutralisation reduced innate IL-17A production and attenuated disease. TNFα, predominantly produced by CD11chigh class II+ CD11b+ CD103- dendritic cells (DCs), synergised with IL-23 to drive IL-17A production by ILCs. <br/>Helicobacter typhlonius (HT) was identified as the key colitigenic bacterium driving TRUC disease. Four-five-four ribosomal RNA gene sequencing demonstrated that HT was consistently present in the faeces of all TRUC mice, but absent from a newly-derived colony of disease-free Tbx21-/- x Rag2-/- mice. Inoculation of pure HT cultures was sufficient to reinstate disease into this new colony of healthy mice. <br/>T-bet impacted on colitis in several ways. In the absence of T-bet, ILCs selectively produced IL-17A and were poor producers of interferon-γ. Second, T-bet deficient DCs produced excess TNFα in comparison with T-bet sufficient DCs. Finally, T-bet was a transcriptional repressor of the Il7ra locus. IL-7R was expressed by ILCs and signalling through this receptor was critical for TRUC disease. Selective IL-7R blockade attenuated disease. Together these data provide new insights into TRUC disease and demonstrate at least some of the mechanisms by which T-bet regulates the interplay between mucosal innate immune cells and the intestinal microbiota."],"dc:identifier":["oai:kclpure.kcl.ac.uk:studenttheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04","https://kclpure.kcl.ac.uk/portal/en/studentTheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04"],"dc:identifier.uri":["https://kclpure.kcl.ac.uk/portal/files/13519072/Studentthesis-Nicholas_Powell_2014.pdf"],"dc:language":["eng"],"dc:publisher.department":["Experimental Immunobiology"],"dc:publisher.institution":["King's College London"],"dc:relation.isreferencedby":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/d78ff348-ac4f-4ef6-b1a9-7e527226bf04"],"dc:title":["Defining the role of the transcription factor T-bet in the innate immune system in intestinal inflammation."],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral Thesis"],"dc:type.qualificationname":["Doctor of Philosophy"]},"updated_at":"2026-07-24T02:44:42Z"}