{"id":{"repo_id":"kings","oai_identifier":"oai:kclpure.kcl.ac.uk:studenttheses/c820f600-610a-49cc-be4e-efce959120b4"},"canonical_url":"https://search.dev.ndltd.org/etd/kings/oai:kclpure.kcl.ac.uk:studenttheses/c820f600-610a-49cc-be4e-efce959120b4","repository":{"repo_id":"kings","name":"King's College London","base_url":"https://kclpure.kcl.ac.uk/ws/oai"},"display":{"title":"In-vitro assessment of modified resin adhesive-tooth interfaces","abstract":"Objectives:
This research aimed to characterize the interfacial characteristics of modified dental resin-based adhesive systems bonded to sound and carious dental tissue. The modification included the incorporation of matrix metalloproteinase (MMP) inhibitors within the primers of these adhesives.
Materials and methods:
Two MMP inhibitors (BB94 and GM6001) were added to three adhesive primers, Optibond FL “OB” (Kerr, USA), Prime&Bond NT “PB” (Dentsply, USA) and G-Bond “GB” (GC, UK) and bonded to sound dentine. The inhibitory effect of the modified adhesive on recombinant MMPs and on sound dentine MMPs was assessed using FRET-based measurement of MMP activity and substrate zymography, respectively. Micro-tensile bond strength and micro-permeability were used to evaluate the modified adhesives’ physical properties. Micro-Raman spectroscopy analysis was validated on carious dentine and it was used to evaluate the interface between the modified OB primer and caries-affected dentine. The inhibitory effect of the modified adhesive on caries-affected dentine was studied using in-situ zymography.
Results:
The fluorometric assay and zymography showed that modified adhesives had high affinity toward both synthetic FRET-peptides and dentine powder substrates, respectively.The immediate micro-tensile bond strength was enhanced for OB (48.0 MPa ± 20.3 SD for BB94 and 42.0 MPa ± 18.7 SD for GM6001) and GB (34.8 MPa ± 19.2 SD for BB94 and 41.7 MPa ± 17.6 SD for GM6001). However, no changes were detected between the control and the inhibitor groups following 3-month storage. Additionally, the micro-permeability of PB and GB showed less dye seepage, to the “hybrid layer” and to the “adhesive”, respectively.
The caries-infected dentine was defined significantly by the KHN (< 20.6), AF (> 14.4 A.U.) and by the relative contribution of the mineral (< 36.4%), Porphyrin fluorescence (> 25.3%) and Infected dentine signal (> 0.3%) Raman clusters. The caries-affected dentine-adhesive interface exhibited more hydrophobic resin (32.8% ± 3.9 SD) that maintained over four-week aging.
Conclusions:
The addition of MMP inhibitors to contemporary dental adhesive systems resulted in modified adhesives that had an enhanced dentine-adhesive interface with inhibited MMP activity. Such properties enhance the clinical performance of adhesive systems.","abstract_html":"Objectives:<br/>This research aimed to characterize the interfacial characteristics of modified dental resin-based adhesive systems bonded to sound and carious dental tissue. The modification included the incorporation of matrix metalloproteinase (MMP) inhibitors within the primers of these adhesives.<br/><br/>Materials and methods:<br/>Two MMP inhibitors (BB94 and GM6001) were added to three adhesive primers, Optibond FL “OB” (Kerr, USA), Prime&Bond NT “PB” (Dentsply, USA) and G-Bond “GB” (GC, UK) and bonded to sound dentine. The inhibitory effect of the modified adhesive on recombinant MMPs and on sound dentine MMPs was assessed using FRET-based measurement of MMP activity and substrate zymography, respectively. Micro-tensile bond strength and micro-permeability were used to evaluate the modified adhesives’ physical properties. Micro-Raman spectroscopy analysis was validated on carious dentine and it was used to evaluate the interface between the modified OB primer and caries-affected dentine. The inhibitory effect of the modified adhesive on caries-affected dentine was studied using in-situ zymography.<br/><br/>Results:<br/>The fluorometric assay and zymography showed that modified adhesives had high affinity toward both synthetic FRET-peptides and dentine powder substrates, respectively.The immediate micro-tensile bond strength was enhanced for OB (48.0 MPa ± 20.3 SD for BB94 and 42.0 MPa ± 18.7 SD for GM6001) and GB (34.8 MPa ± 19.2 SD for BB94 and 41.7 MPa ± 17.6 SD for GM6001). However, no changes were detected between the control and the inhibitor groups following 3-month storage. Additionally, the micro-permeability of PB and GB showed less dye seepage, to the “hybrid layer” and to the “adhesive”, respectively.<br/>The caries-infected dentine was defined significantly by the KHN (< 20.6), AF (> 14.4 A.U.) and by the relative contribution of the mineral (< 36.4%), Porphyrin fluorescence (> 25.3%) and Infected dentine signal (> 0.3%) Raman clusters. The caries-affected dentine-adhesive interface exhibited more hydrophobic resin (32.8% ± 3.9 SD) that maintained over four-week aging.<br/><br/>Conclusions:<br/>The addition of MMP inhibitors to contemporary dental adhesive systems resulted in modified adhesives that had an enhanced dentine-adhesive interface with inhibited MMP activity. Such properties enhance the clinical performance of adhesive systems.","abstract_has_math":false,"creators":["Almahdy, Ahmed"],"institution":"King's College London","degree_name":"Doctor of Philosophy","degree_level":"Doctoral Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Banerjee, Avijit","Watson, Timothy F"],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-7-1","date_published":"2013-7-1","updated_at":"2026-07-24T02:44:31Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/c820f600-610a-49cc-be4e-efce959120b4"],"render_values":[{"text":"oai:kclpure.kcl.ac.uk:studenttheses/c820f600-610a-49cc-be4e-efce959120b4","href":null,"code":true}]}]},"links":{"outbound_url":"https://kclpure.kcl.ac.uk/portal/en/studentTheses/c820f600-610a-49cc-be4e-efce959120b4","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Banerjee, Avijit","Watson, Timothy F"]},{"key":"dc:creator","label":"Author","values":["Almahdy, Ahmed"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-7-1"]},{"key":"dc:date.issued","label":"Date","values":["2013-7-1"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Conservative Dentistry (including Endodontics)"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["King's College London"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/c820f600-610a-49cc-be4e-efce959120b4"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/c820f600-610a-49cc-be4e-efce959120b4","https://kclpure.kcl.ac.uk/portal/en/studentTheses/c820f600-610a-49cc-be4e-efce959120b4"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://kclpure.kcl.ac.uk/portal/files/12624516/Studentthesis-Ahmed_Almahdy_2013.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Objectives:
This research aimed to characterize the interfacial characteristics of modified dental resin-based adhesive systems bonded to sound and carious dental tissue. The modification included the incorporation of matrix metalloproteinase (MMP) inhibitors within the primers of these adhesives.
Materials and methods:
Two MMP inhibitors (BB94 and GM6001) were added to three adhesive primers, Optibond FL “OB” (Kerr, USA), Prime&Bond NT “PB” (Dentsply, USA) and G-Bond “GB” (GC, UK) and bonded to sound dentine. The inhibitory effect of the modified adhesive on recombinant MMPs and on sound dentine MMPs was assessed using FRET-based measurement of MMP activity and substrate zymography, respectively. Micro-tensile bond strength and micro-permeability were used to evaluate the modified adhesives’ physical properties. Micro-Raman spectroscopy analysis was validated on carious dentine and it was used to evaluate the interface between the modified OB primer and caries-affected dentine. The inhibitory effect of the modified adhesive on caries-affected dentine was studied using in-situ zymography.
Results:
The fluorometric assay and zymography showed that modified adhesives had high affinity toward both synthetic FRET-peptides and dentine powder substrates, respectively.The immediate micro-tensile bond strength was enhanced for OB (48.0 MPa ± 20.3 SD for BB94 and 42.0 MPa ± 18.7 SD for GM6001) and GB (34.8 MPa ± 19.2 SD for BB94 and 41.7 MPa ± 17.6 SD for GM6001). However, no changes were detected between the control and the inhibitor groups following 3-month storage. Additionally, the micro-permeability of PB and GB showed less dye seepage, to the “hybrid layer” and to the “adhesive”, respectively.
The caries-infected dentine was defined significantly by the KHN (< 20.6), AF (> 14.4 A.U.) and by the relative contribution of the mineral (< 36.4%), Porphyrin fluorescence (> 25.3%) and Infected dentine signal (> 0.3%) Raman clusters. The caries-affected dentine-adhesive interface exhibited more hydrophobic resin (32.8% ± 3.9 SD) that maintained over four-week aging.
Conclusions:
The addition of MMP inhibitors to contemporary dental adhesive systems resulted in modified adhesives that had an enhanced dentine-adhesive interface with inhibited MMP activity. Such properties enhance the clinical performance of adhesive systems."]},{"key":"dc:title","label":"Title","values":["In-vitro assessment of modified resin adhesive-tooth interfaces"]}]}],"canonical_facts":{"dc:contributor.advisor":["Banerjee, Avijit","Watson, Timothy F"],"dc:creator":["Almahdy, Ahmed"],"dc:date":["2013-7-1"],"dc:date.issued":["2013-7-1"],"dc:description.abstract":["Objectives:
This research aimed to characterize the interfacial characteristics of modified dental resin-based adhesive systems bonded to sound and carious dental tissue. The modification included the incorporation of matrix metalloproteinase (MMP) inhibitors within the primers of these adhesives.
Materials and methods:
Two MMP inhibitors (BB94 and GM6001) were added to three adhesive primers, Optibond FL “OB” (Kerr, USA), Prime&Bond NT “PB” (Dentsply, USA) and G-Bond “GB” (GC, UK) and bonded to sound dentine. The inhibitory effect of the modified adhesive on recombinant MMPs and on sound dentine MMPs was assessed using FRET-based measurement of MMP activity and substrate zymography, respectively. Micro-tensile bond strength and micro-permeability were used to evaluate the modified adhesives’ physical properties. Micro-Raman spectroscopy analysis was validated on carious dentine and it was used to evaluate the interface between the modified OB primer and caries-affected dentine. The inhibitory effect of the modified adhesive on caries-affected dentine was studied using in-situ zymography.
Results:
The fluorometric assay and zymography showed that modified adhesives had high affinity toward both synthetic FRET-peptides and dentine powder substrates, respectively.The immediate micro-tensile bond strength was enhanced for OB (48.0 MPa ± 20.3 SD for BB94 and 42.0 MPa ± 18.7 SD for GM6001) and GB (34.8 MPa ± 19.2 SD for BB94 and 41.7 MPa ± 17.6 SD for GM6001). However, no changes were detected between the control and the inhibitor groups following 3-month storage. Additionally, the micro-permeability of PB and GB showed less dye seepage, to the “hybrid layer” and to the “adhesive”, respectively.
The caries-infected dentine was defined significantly by the KHN (< 20.6), AF (> 14.4 A.U.) and by the relative contribution of the mineral (< 36.4%), Porphyrin fluorescence (> 25.3%) and Infected dentine signal (> 0.3%) Raman clusters. The caries-affected dentine-adhesive interface exhibited more hydrophobic resin (32.8% ± 3.9 SD) that maintained over four-week aging.
Conclusions:
The addition of MMP inhibitors to contemporary dental adhesive systems resulted in modified adhesives that had an enhanced dentine-adhesive interface with inhibited MMP activity. Such properties enhance the clinical performance of adhesive systems."],"dc:identifier":["oai:kclpure.kcl.ac.uk:studenttheses/c820f600-610a-49cc-be4e-efce959120b4","https://kclpure.kcl.ac.uk/portal/en/studentTheses/c820f600-610a-49cc-be4e-efce959120b4"],"dc:identifier.uri":["https://kclpure.kcl.ac.uk/portal/files/12624516/Studentthesis-Ahmed_Almahdy_2013.pdf"],"dc:language":["eng"],"dc:publisher.department":["Conservative Dentistry (including Endodontics)"],"dc:publisher.institution":["King's College London"],"dc:relation.isreferencedby":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/c820f600-610a-49cc-be4e-efce959120b4"],"dc:title":["In-vitro assessment of modified resin adhesive-tooth interfaces"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral Thesis"],"dc:type.qualificationname":["Doctor of Philosophy"]},"updated_at":"2026-07-24T02:44:31Z"}