{"id":{"repo_id":"kings","oai_identifier":"oai:kclpure.kcl.ac.uk:studenttheses/74f5dffc-db59-478e-bf87-46b476092e31"},"canonical_url":"https://search.dev.ndltd.org/etd/kings/oai:kclpure.kcl.ac.uk:studenttheses/74f5dffc-db59-478e-bf87-46b476092e31","repository":{"repo_id":"kings","name":"King's College London","base_url":"https://kclpure.kcl.ac.uk/ws/oai"},"display":{"title":"Soluble urokinase plasminogen activator receptor in venous ulcers","abstract":"Compression therapy remains the only treatment option for venous ulcers with
<100% success. A better understanding of the mechanisms regulating venous
ulcer healing might give rise to better treatments.
Urokinsae plasminogen activator receptor (uPAR), a GPI anchored 3‐domain
protein exists either attached to cell wall or in soluble forms (suPARI‐III, suPARI
& suPARII‐III fragments). Cleavage of uPAR results in a number of nonproteolytic
functions that may be important in wound healing. Our aim was to
determine whether suPAR and its fragments are present in the environment of
venous ulcers and whether this receptor has a role in ulcer‐healing.
Ulcer exudates and ulcer tissue biopsies were obtained from patients with
confirmed venous leg ulcers. Venous ulcers that healed within 6months of
compression therapy were classified as healers (H) while those that did not heal
in this time were defined as non‐healers (NH). Time resolved fluorescence
immunoassays (TR‐FIA) were validated for measurement of suPAR fragments.
TR‐FIAs specifically detected their antigens in wound exudates, but not in tissue
homogenates. All forms of suPAR fragments were detected within venous ulcer
exudates. Levels of all 3 suPAR fragments were significantly higher in exudates
obtained from H compared to NH. ELISA analysis of uPA and plasminogen
acitvator inhibitor (PAI‐1) antigen revealed that levels were similar in ulcer
exudates and tissue homogenates from H and NH.
9
Treatment of scratched keratinocyte cultures with exudates from H resulted in a
greater coverage of the scratch compared with NH. Depletion of suPAR from
exudates obtained from H resulted in cell death.
Results from this study have shown the presence of suPAR fragments within
venous ulcers with higher presence of suPAR fragments in H compared to NH.
This data suggests a role of suPAR and its fragments in ulcer healing, although
the precise mechanisms involved remain to be identified. Measurement of
suPAR fragments may provide a prognostic indicator and a therapeutic target
for the treatment of venous ulcers.","abstract_html":"Compression therapy remains the only treatment option for venous ulcers with<br/>&lt;100% success. A better understanding of the mechanisms regulating venous<br/>ulcer healing might give rise to better treatments.<br/>Urokinsae plasminogen activator receptor (uPAR), a GPI anchored 3‐domain<br/>protein exists either attached to cell wall or in soluble forms (suPARI‐III, suPARI<br/>&amp; suPARII‐III fragments). Cleavage of uPAR results in a number of nonproteolytic<br/>functions that may be important in wound healing. Our aim was to<br/>determine whether suPAR and its fragments are present in the environment of<br/>venous ulcers and whether this receptor has a role in ulcer‐healing.<br/>Ulcer exudates and ulcer tissue biopsies were obtained from patients with<br/>confirmed venous leg ulcers. Venous ulcers that healed within 6months of<br/>compression therapy were classified as healers (H) while those that did not heal<br/>in this time were defined as non‐healers (NH). Time resolved fluorescence<br/>immunoassays (TR‐FIA) were validated for measurement of suPAR fragments.<br/>TR‐FIAs specifically detected their antigens in wound exudates, but not in tissue<br/>homogenates. All forms of suPAR fragments were detected within venous ulcer<br/>exudates. Levels of all 3 suPAR fragments were significantly higher in exudates<br/>obtained from H compared to NH. ELISA analysis of uPA and plasminogen<br/>acitvator inhibitor (PAI‐1) antigen revealed that levels were similar in ulcer<br/>exudates and tissue homogenates from H and NH.<br/>9<br/>Treatment of scratched keratinocyte cultures with exudates from H resulted in a<br/>greater coverage of the scratch compared with NH. Depletion of suPAR from<br/>exudates obtained from H resulted in cell death.<br/>Results from this study have shown the presence of suPAR fragments within<br/>venous ulcers with higher presence of suPAR fragments in H compared to NH.<br/>This data suggests a role of suPAR and its fragments in ulcer healing, although<br/>the precise mechanisms involved remain to be identified. Measurement of<br/>suPAR fragments may provide a prognostic indicator and a therapeutic target<br/>for the treatment of venous ulcers.","abstract_has_math":false,"creators":["Ahmad, Anwar"],"institution":"King's College London","degree_name":"Doctor of Medicine by Research","degree_level":"Doctoral Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Burnand, Kevin Guiver","Smith, Alberto"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-1-1","date_published":"2014-1-1","updated_at":"2026-07-24T02:44:31Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/74f5dffc-db59-478e-bf87-46b476092e31"],"render_values":[{"text":"oai:kclpure.kcl.ac.uk:studenttheses/74f5dffc-db59-478e-bf87-46b476092e31","href":null,"code":true}]}]},"links":{"outbound_url":"https://kclpure.kcl.ac.uk/portal/en/studentTheses/74f5dffc-db59-478e-bf87-46b476092e31","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Burnand, Kevin Guiver","Smith, Alberto"]},{"key":"dc:creator","label":"Author","values":["Ahmad, Anwar"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-1-1"]},{"key":"dc:date.issued","label":"Date","values":["2014-1-1"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Vascular Risk & Surgery"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["King's College London"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/74f5dffc-db59-478e-bf87-46b476092e31"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Medicine by Research"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/74f5dffc-db59-478e-bf87-46b476092e31","https://kclpure.kcl.ac.uk/portal/en/studentTheses/74f5dffc-db59-478e-bf87-46b476092e31"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://kclpure.kcl.ac.uk/portal/files/12375460/Studentthesis-Anwar_Ahmad_2014.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Compression therapy remains the only treatment option for venous ulcers with
<100% success. A better understanding of the mechanisms regulating venous
ulcer healing might give rise to better treatments.
Urokinsae plasminogen activator receptor (uPAR), a GPI anchored 3‐domain
protein exists either attached to cell wall or in soluble forms (suPARI‐III, suPARI
& suPARII‐III fragments). Cleavage of uPAR results in a number of nonproteolytic
functions that may be important in wound healing. Our aim was to
determine whether suPAR and its fragments are present in the environment of
venous ulcers and whether this receptor has a role in ulcer‐healing.
Ulcer exudates and ulcer tissue biopsies were obtained from patients with
confirmed venous leg ulcers. Venous ulcers that healed within 6months of
compression therapy were classified as healers (H) while those that did not heal
in this time were defined as non‐healers (NH). Time resolved fluorescence
immunoassays (TR‐FIA) were validated for measurement of suPAR fragments.
TR‐FIAs specifically detected their antigens in wound exudates, but not in tissue
homogenates. All forms of suPAR fragments were detected within venous ulcer
exudates. Levels of all 3 suPAR fragments were significantly higher in exudates
obtained from H compared to NH. ELISA analysis of uPA and plasminogen
acitvator inhibitor (PAI‐1) antigen revealed that levels were similar in ulcer
exudates and tissue homogenates from H and NH.
9
Treatment of scratched keratinocyte cultures with exudates from H resulted in a
greater coverage of the scratch compared with NH. Depletion of suPAR from
exudates obtained from H resulted in cell death.
Results from this study have shown the presence of suPAR fragments within
venous ulcers with higher presence of suPAR fragments in H compared to NH.
This data suggests a role of suPAR and its fragments in ulcer healing, although
the precise mechanisms involved remain to be identified. Measurement of
suPAR fragments may provide a prognostic indicator and a therapeutic target
for the treatment of venous ulcers."]},{"key":"dc:title","label":"Title","values":["Soluble urokinase plasminogen activator receptor in venous ulcers"]}]}],"canonical_facts":{"dc:contributor.advisor":["Burnand, Kevin Guiver","Smith, Alberto"],"dc:creator":["Ahmad, Anwar"],"dc:date":["2014-1-1"],"dc:date.issued":["2014-1-1"],"dc:description.abstract":["Compression therapy remains the only treatment option for venous ulcers with
<100% success. A better understanding of the mechanisms regulating venous
ulcer healing might give rise to better treatments.
Urokinsae plasminogen activator receptor (uPAR), a GPI anchored 3‐domain
protein exists either attached to cell wall or in soluble forms (suPARI‐III, suPARI
& suPARII‐III fragments). Cleavage of uPAR results in a number of nonproteolytic
functions that may be important in wound healing. Our aim was to
determine whether suPAR and its fragments are present in the environment of
venous ulcers and whether this receptor has a role in ulcer‐healing.
Ulcer exudates and ulcer tissue biopsies were obtained from patients with
confirmed venous leg ulcers. Venous ulcers that healed within 6months of
compression therapy were classified as healers (H) while those that did not heal
in this time were defined as non‐healers (NH). Time resolved fluorescence
immunoassays (TR‐FIA) were validated for measurement of suPAR fragments.
TR‐FIAs specifically detected their antigens in wound exudates, but not in tissue
homogenates. All forms of suPAR fragments were detected within venous ulcer
exudates. Levels of all 3 suPAR fragments were significantly higher in exudates
obtained from H compared to NH. ELISA analysis of uPA and plasminogen
acitvator inhibitor (PAI‐1) antigen revealed that levels were similar in ulcer
exudates and tissue homogenates from H and NH.
9
Treatment of scratched keratinocyte cultures with exudates from H resulted in a
greater coverage of the scratch compared with NH. Depletion of suPAR from
exudates obtained from H resulted in cell death.
Results from this study have shown the presence of suPAR fragments within
venous ulcers with higher presence of suPAR fragments in H compared to NH.
This data suggests a role of suPAR and its fragments in ulcer healing, although
the precise mechanisms involved remain to be identified. Measurement of
suPAR fragments may provide a prognostic indicator and a therapeutic target
for the treatment of venous ulcers."],"dc:identifier":["oai:kclpure.kcl.ac.uk:studenttheses/74f5dffc-db59-478e-bf87-46b476092e31","https://kclpure.kcl.ac.uk/portal/en/studentTheses/74f5dffc-db59-478e-bf87-46b476092e31"],"dc:identifier.uri":["https://kclpure.kcl.ac.uk/portal/files/12375460/Studentthesis-Anwar_Ahmad_2014.pdf"],"dc:language":["eng"],"dc:publisher.department":["Vascular Risk & Surgery"],"dc:publisher.institution":["King's College London"],"dc:relation.isreferencedby":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/74f5dffc-db59-478e-bf87-46b476092e31"],"dc:title":["Soluble urokinase plasminogen activator receptor in venous ulcers"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral Thesis"],"dc:type.qualificationname":["Doctor of Medicine by Research"]},"updated_at":"2026-07-24T02:44:31Z"}