{"id":{"repo_id":"kings","oai_identifier":"oai:kclpure.kcl.ac.uk:studenttheses/53e3fda4-26d6-4cbb-8c03-e667bab85705"},"canonical_url":"https://search.dev.ndltd.org/etd/kings/oai:kclpure.kcl.ac.uk:studenttheses/53e3fda4-26d6-4cbb-8c03-e667bab85705","repository":{"repo_id":"kings","name":"King's College London","base_url":"https://kclpure.kcl.ac.uk/ws/oai"},"display":{"title":"The Role of Transitional B cells in Kidney Transplantation Tolerance","abstract":"Previous studies aimed at identifying biomarkers of tolerance in kidney transplant patients have revealed an expansion of peripheral blood B cells and over- expression of B cell-related genes. In humans, Memory, Naïve and Transitional B cells are the main B cell subsets found in circulation, and it is has been shown that the Transitional subset, defined by its regulatory properties, is expanded in tolerant recipients compared to non-tolerant kidney transplant patients. However, the role this population plays in kidney transplantation tolerance remains unclear. Here, we report three different mechanisms to explain the contribution of B cells in transplantation tolerance.
Kidney transplant patients were divided into five groups; tolerant, stable, monotherapy, patients who lost tolerance and chronic rejector. In addition, this study also included a group of age/gender-matched healthy volunteers. B cells from each group of patients were tested for antigen presentation, antibody production, cytokine production, and co-stimulatory function.
B cells from tolerant patients produced higher levels of IL-10 and lower levels of TNF-α than B cells from chronic rejector after CD40 and CpG activation. Moreover, B lymphocytes from tolerant patients also exhibited a failure in the BCR signalling pathway, suggesting a certain degree of anergy or responsiveness by these cells. Donor- specific assays revealed that B cells from tolerant patients were inefficient to recognise donor-antigens, compared to B cells from chronic rejector. This impairment prevented the triggering of the Th1 response by recipient CD4+ T cells and donor-specific antibody production by Plasma cells. Finally, Transitional B cells were the lowest CD4+ T cell- activating cells, compared to Naïve and Memory B cells. This reduced CD4+ T cell activation was due to low cell viability, reduced CD86 expression and high IL-10 production.
In conclusion, these data suggest that B cells from kidney tolerant recipients contributed to maintaining organ acceptance and graft survival by donor-specific and non-specific regulatory properties exhibited by all their subsets, especially Transitional B cells.
","abstract_html":"Previous studies aimed at identifying biomarkers of tolerance in kidney transplant patients have revealed an expansion of peripheral blood B cells and over- expression of B cell-related genes. In humans, Memory, Naïve and Transitional B cells are the main B cell subsets found in circulation, and it is has been shown that the Transitional subset, defined by its regulatory properties, is expanded in tolerant recipients compared to non-tolerant kidney transplant patients. However, the role this population plays in kidney transplantation tolerance remains unclear. Here, we report three different mechanisms to explain the contribution of B cells in transplantation tolerance.<br/><br/>Kidney transplant patients were divided into five groups; tolerant, stable, monotherapy, patients who lost tolerance and chronic rejector. In addition, this study also included a group of age/gender-matched healthy volunteers. B cells from each group of patients were tested for antigen presentation, antibody production, cytokine production, and co-stimulatory function.<br/><br/>B cells from tolerant patients produced higher levels of IL-10 and lower levels of TNF-α than B cells from chronic rejector after CD40 and CpG activation. Moreover, B lymphocytes from tolerant patients also exhibited a failure in the BCR signalling pathway, suggesting a certain degree of anergy or responsiveness by these cells. Donor- specific assays revealed that B cells from tolerant patients were inefficient to recognise donor-antigens, compared to B cells from chronic rejector. This impairment prevented the triggering of the Th1 response by recipient CD4+ T cells and donor-specific antibody production by Plasma cells. Finally, Transitional B cells were the lowest CD4+ T cell- activating cells, compared to Naïve and Memory B cells. This reduced CD4+ T cell activation was due to low cell viability, reduced CD86 expression and high IL-10 production.<br/><br/>In conclusion, these data suggest that B cells from kidney tolerant recipients contributed to maintaining organ acceptance and graft survival by donor-specific and non-specific regulatory properties exhibited by all their subsets, especially Transitional B cells.<br/>","abstract_has_math":false,"creators":["Nova Lamperti, Estefania"],"institution":"King's College London","degree_name":"Doctor of Philosophy","degree_level":"Doctoral Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Lombardi, Giovanna","Hernandez Fuentes, Maria Puerto"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-3-1","date_published":"2014-3-1","updated_at":"2026-07-24T02:44:42Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/53e3fda4-26d6-4cbb-8c03-e667bab85705"],"render_values":[{"text":"oai:kclpure.kcl.ac.uk:studenttheses/53e3fda4-26d6-4cbb-8c03-e667bab85705","href":null,"code":true}]}]},"links":{"outbound_url":"https://kclpure.kcl.ac.uk/portal/en/studentTheses/53e3fda4-26d6-4cbb-8c03-e667bab85705","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Lombardi, Giovanna","Hernandez Fuentes, Maria Puerto"]},{"key":"dc:creator","label":"Author","values":["Nova Lamperti, Estefania"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-3-1"]},{"key":"dc:date.issued","label":"Date","values":["2014-3-1"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Experimental Immunobiology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["King's College London"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/53e3fda4-26d6-4cbb-8c03-e667bab85705"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/53e3fda4-26d6-4cbb-8c03-e667bab85705","https://kclpure.kcl.ac.uk/portal/en/studentTheses/53e3fda4-26d6-4cbb-8c03-e667bab85705"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://kclpure.kcl.ac.uk/portal/files/13320015/Studentthesis-Estefania_Nova%20Lamperti_2014.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Previous studies aimed at identifying biomarkers of tolerance in kidney transplant patients have revealed an expansion of peripheral blood B cells and over- expression of B cell-related genes. In humans, Memory, Naïve and Transitional B cells are the main B cell subsets found in circulation, and it is has been shown that the Transitional subset, defined by its regulatory properties, is expanded in tolerant recipients compared to non-tolerant kidney transplant patients. However, the role this population plays in kidney transplantation tolerance remains unclear. Here, we report three different mechanisms to explain the contribution of B cells in transplantation tolerance.
Kidney transplant patients were divided into five groups; tolerant, stable, monotherapy, patients who lost tolerance and chronic rejector. In addition, this study also included a group of age/gender-matched healthy volunteers. B cells from each group of patients were tested for antigen presentation, antibody production, cytokine production, and co-stimulatory function.
B cells from tolerant patients produced higher levels of IL-10 and lower levels of TNF-α than B cells from chronic rejector after CD40 and CpG activation. Moreover, B lymphocytes from tolerant patients also exhibited a failure in the BCR signalling pathway, suggesting a certain degree of anergy or responsiveness by these cells. Donor- specific assays revealed that B cells from tolerant patients were inefficient to recognise donor-antigens, compared to B cells from chronic rejector. This impairment prevented the triggering of the Th1 response by recipient CD4+ T cells and donor-specific antibody production by Plasma cells. Finally, Transitional B cells were the lowest CD4+ T cell- activating cells, compared to Naïve and Memory B cells. This reduced CD4+ T cell activation was due to low cell viability, reduced CD86 expression and high IL-10 production.
In conclusion, these data suggest that B cells from kidney tolerant recipients contributed to maintaining organ acceptance and graft survival by donor-specific and non-specific regulatory properties exhibited by all their subsets, especially Transitional B cells.
"]},{"key":"dc:title","label":"Title","values":["The Role of Transitional B cells in Kidney Transplantation Tolerance"]}]}],"canonical_facts":{"dc:contributor.advisor":["Lombardi, Giovanna","Hernandez Fuentes, Maria Puerto"],"dc:creator":["Nova Lamperti, Estefania"],"dc:date":["2014-3-1"],"dc:date.issued":["2014-3-1"],"dc:description.abstract":["Previous studies aimed at identifying biomarkers of tolerance in kidney transplant patients have revealed an expansion of peripheral blood B cells and over- expression of B cell-related genes. In humans, Memory, Naïve and Transitional B cells are the main B cell subsets found in circulation, and it is has been shown that the Transitional subset, defined by its regulatory properties, is expanded in tolerant recipients compared to non-tolerant kidney transplant patients. However, the role this population plays in kidney transplantation tolerance remains unclear. Here, we report three different mechanisms to explain the contribution of B cells in transplantation tolerance.
Kidney transplant patients were divided into five groups; tolerant, stable, monotherapy, patients who lost tolerance and chronic rejector. In addition, this study also included a group of age/gender-matched healthy volunteers. B cells from each group of patients were tested for antigen presentation, antibody production, cytokine production, and co-stimulatory function.
B cells from tolerant patients produced higher levels of IL-10 and lower levels of TNF-α than B cells from chronic rejector after CD40 and CpG activation. Moreover, B lymphocytes from tolerant patients also exhibited a failure in the BCR signalling pathway, suggesting a certain degree of anergy or responsiveness by these cells. Donor- specific assays revealed that B cells from tolerant patients were inefficient to recognise donor-antigens, compared to B cells from chronic rejector. This impairment prevented the triggering of the Th1 response by recipient CD4+ T cells and donor-specific antibody production by Plasma cells. Finally, Transitional B cells were the lowest CD4+ T cell- activating cells, compared to Naïve and Memory B cells. This reduced CD4+ T cell activation was due to low cell viability, reduced CD86 expression and high IL-10 production.
In conclusion, these data suggest that B cells from kidney tolerant recipients contributed to maintaining organ acceptance and graft survival by donor-specific and non-specific regulatory properties exhibited by all their subsets, especially Transitional B cells.
"],"dc:identifier":["oai:kclpure.kcl.ac.uk:studenttheses/53e3fda4-26d6-4cbb-8c03-e667bab85705","https://kclpure.kcl.ac.uk/portal/en/studentTheses/53e3fda4-26d6-4cbb-8c03-e667bab85705"],"dc:identifier.uri":["https://kclpure.kcl.ac.uk/portal/files/13320015/Studentthesis-Estefania_Nova%20Lamperti_2014.pdf"],"dc:language":["eng"],"dc:publisher.department":["Experimental Immunobiology"],"dc:publisher.institution":["King's College London"],"dc:relation.isreferencedby":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/53e3fda4-26d6-4cbb-8c03-e667bab85705"],"dc:title":["The Role of Transitional B cells in Kidney Transplantation Tolerance"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral Thesis"],"dc:type.qualificationname":["Doctor of Philosophy"]},"updated_at":"2026-07-24T02:44:42Z"}