{"id":{"repo_id":"kings","oai_identifier":"oai:kclpure.kcl.ac.uk:studenttheses/46cfba10-5ebb-41a2-9db3-1892daa17e76"},"canonical_url":"https://search.dev.ndltd.org/etd/kings/oai:kclpure.kcl.ac.uk:studenttheses/46cfba10-5ebb-41a2-9db3-1892daa17e76","repository":{"repo_id":"kings","name":"King's College London","base_url":"https://kclpure.kcl.ac.uk/ws/oai"},"display":{"title":"Hybridisation of dental hard tissues with modified adhesive systems: therapeutic impact of bioactive silicate compounds on bonding to dentine","abstract":"The first section of this work is a review of the literature necessary to understand the objectives of the project; it includes general information about dental adhesive technology as well as adhesion testing, about dentine hybridisation and about the drawbacks of contemporary bonding systems. Several studies revealed excellent immediate and short-term bonding effectiveness of etch-and-rinse adhesives, yet substantial reductions in resin- dentine bond strength occur after ageing. Degenerative phenomena involve hydrolysis of suboptimally polymerised hydrophilic resin components and degradation of mineral-deprived water-rich resin-sparse collagen matrices by matrix metalloproteinases and cysteine cathepsins.
Silicate compounds, including calcium/sodium phosphosilicates, such as commercially available bioactive glass, and calcium-silicate Portland-derived cements are known to promote the formation of apatite in aqueous environments that contain calcium and phosphate (e.g. saliva); thus, we have raised questions about whether their presence at the bonded interface could increase the in vitro durability of resin-dentine bonds through crystal formation and self-sealing, in the presence of phosphate buffered saline or simulated body fluid solutions.
In answering these questions, the objectives were accomplished by employing Bioglass® 45S5 in etch-and-rinse bonding procedures either (i) included within the composition of a resin adhesive as a tailored micro-filler, or (ii) applied directly onto acid-etched wetted dentine. Alternative light-curable methacrylate-based agents containing (iii) three modified calcium-silicates derived from ordinary Portland cement were also tested.
Confirming the relative success of bioactive materials incorporated in the dentine bonding procedures required assessment of the potential to reduce nano-leakage, as well as their effect upon the strength of the bond over time.
In order to explore these possibilities, which have not been previously investigated, a combination of methods were applied in the second experimental section. Bond strength variations were quantified using the microtensile test while scanning electron microscopy, confocal laser scanning microscopy and Knoop micro-indentation analysis were used to evaluate optically and mechanically adjustments to mineral and water content within the resin bonded-dentine interface. Initially, high microtensile values were achieved in each tested group. All the resin-dentine interfaces created with bonding agents containing micro-fillers showed an evident reduction of nano-leakage and mineral deposition after the ageing period. However, only adhesive systems containing Bioglass and two modified Portland cement-based micro- fillers were found to reduce nano-leakage with no negative effects on bond strength. Furthermore, specimens created with the same experimental adhesives did not restore micro-hardness to the level of sound dentine but were able to maintain statistically unaltered Knoop values.
The second section is also composed of a set of preliminary studies that involved the use of up-to-date spectroscopic (attenuated total reflection Fourier transform infrared spectroscopy) and thermoanalytical (differential scanning calorimetry) techniques to predict the chemical-physical properties and apatite- forming ability of the novel ion-leachable hybrid materials. Lastly, the overall conclusions of the present work and directions for future research are discussed.
","abstract_html":"The first section of this work is a review of the literature necessary to understand the objectives of the project; it includes general information about dental adhesive technology as well as adhesion testing, about dentine hybridisation and about the drawbacks of contemporary bonding systems. Several studies revealed excellent immediate and short-term bonding effectiveness of etch-and-rinse adhesives, yet substantial reductions in resin- dentine bond strength occur after ageing. Degenerative phenomena involve hydrolysis of suboptimally polymerised hydrophilic resin components and degradation of mineral-deprived water-rich resin-sparse collagen matrices by matrix metalloproteinases and cysteine cathepsins.<br/>Silicate compounds, including calcium/sodium phosphosilicates, such as commercially available bioactive glass, and calcium-silicate Portland-derived cements are known to promote the formation of apatite in aqueous environments that contain calcium and phosphate (e.g. saliva); thus, we have raised questions about whether their presence at the bonded interface could increase the in vitro durability of resin-dentine bonds through crystal formation and self-sealing, in the presence of phosphate buffered saline or simulated body fluid solutions.<br/>In answering these questions, the objectives were accomplished by employing Bioglass® 45S5 in etch-and-rinse bonding procedures either (i) included within the composition of a resin adhesive as a tailored micro-filler, or (ii) applied directly onto acid-etched wetted dentine. Alternative light-curable methacrylate-based agents containing (iii) three modified calcium-silicates derived from ordinary Portland cement were also tested.<br/>Confirming the relative success of bioactive materials incorporated in the dentine bonding procedures required assessment of the potential to reduce nano-leakage, as well as their effect upon the strength of the bond over time.<br/>In order to explore these possibilities, which have not been previously investigated, a combination of methods were applied in the second experimental section. Bond strength variations were quantified using the microtensile test while scanning electron microscopy, confocal laser scanning microscopy and Knoop micro-indentation analysis were used to evaluate optically and mechanically adjustments to mineral and water content within the resin bonded-dentine interface. Initially, high microtensile values were achieved in each tested group. All the resin-dentine interfaces created with bonding agents containing micro-fillers showed an evident reduction of nano-leakage and mineral deposition after the ageing period. However, only adhesive systems containing Bioglass and two modified Portland cement-based micro- fillers were found to reduce nano-leakage with no negative effects on bond strength. Furthermore, specimens created with the same experimental adhesives did not restore micro-hardness to the level of sound dentine but were able to maintain statistically unaltered Knoop values.<br/>The second section is also composed of a set of preliminary studies that involved the use of up-to-date spectroscopic (attenuated total reflection Fourier transform infrared spectroscopy) and thermoanalytical (differential scanning calorimetry) techniques to predict the chemical-physical properties and apatite- forming ability of the novel ion-leachable hybrid materials. Lastly, the overall conclusions of the present work and directions for future research are discussed.<br/>","abstract_has_math":false,"creators":["Corrado Profeta, Andrea"],"institution":"King's College London","degree_name":"Doctor of Philosophy","degree_level":"Doctoral Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Foxton, Richard Mark"],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-7-1","date_published":"2013-7-1","updated_at":"2026-07-24T02:44:31Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/46cfba10-5ebb-41a2-9db3-1892daa17e76"],"render_values":[{"text":"oai:kclpure.kcl.ac.uk:studenttheses/46cfba10-5ebb-41a2-9db3-1892daa17e76","href":null,"code":true}]}]},"links":{"outbound_url":"https://kclpure.kcl.ac.uk/portal/en/studentTheses/46cfba10-5ebb-41a2-9db3-1892daa17e76","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Foxton, Richard Mark"]},{"key":"dc:creator","label":"Author","values":["Corrado Profeta, Andrea"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2013-7-1"]},{"key":"dc:date.issued","label":"Date","values":["2013-7-1"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Conservative Dentistry (including Endodontics)"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["King's College London"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/46cfba10-5ebb-41a2-9db3-1892daa17e76"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:kclpure.kcl.ac.uk:studenttheses/46cfba10-5ebb-41a2-9db3-1892daa17e76","https://kclpure.kcl.ac.uk/portal/en/studentTheses/46cfba10-5ebb-41a2-9db3-1892daa17e76"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://kclpure.kcl.ac.uk/portal/files/11772676/Studentthesis-Andrea_Corrado%20Profeta_2013.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The first section of this work is a review of the literature necessary to understand the objectives of the project; it includes general information about dental adhesive technology as well as adhesion testing, about dentine hybridisation and about the drawbacks of contemporary bonding systems. Several studies revealed excellent immediate and short-term bonding effectiveness of etch-and-rinse adhesives, yet substantial reductions in resin- dentine bond strength occur after ageing. Degenerative phenomena involve hydrolysis of suboptimally polymerised hydrophilic resin components and degradation of mineral-deprived water-rich resin-sparse collagen matrices by matrix metalloproteinases and cysteine cathepsins.
Silicate compounds, including calcium/sodium phosphosilicates, such as commercially available bioactive glass, and calcium-silicate Portland-derived cements are known to promote the formation of apatite in aqueous environments that contain calcium and phosphate (e.g. saliva); thus, we have raised questions about whether their presence at the bonded interface could increase the in vitro durability of resin-dentine bonds through crystal formation and self-sealing, in the presence of phosphate buffered saline or simulated body fluid solutions.
In answering these questions, the objectives were accomplished by employing Bioglass® 45S5 in etch-and-rinse bonding procedures either (i) included within the composition of a resin adhesive as a tailored micro-filler, or (ii) applied directly onto acid-etched wetted dentine. Alternative light-curable methacrylate-based agents containing (iii) three modified calcium-silicates derived from ordinary Portland cement were also tested.
Confirming the relative success of bioactive materials incorporated in the dentine bonding procedures required assessment of the potential to reduce nano-leakage, as well as their effect upon the strength of the bond over time.
In order to explore these possibilities, which have not been previously investigated, a combination of methods were applied in the second experimental section. Bond strength variations were quantified using the microtensile test while scanning electron microscopy, confocal laser scanning microscopy and Knoop micro-indentation analysis were used to evaluate optically and mechanically adjustments to mineral and water content within the resin bonded-dentine interface. Initially, high microtensile values were achieved in each tested group. All the resin-dentine interfaces created with bonding agents containing micro-fillers showed an evident reduction of nano-leakage and mineral deposition after the ageing period. However, only adhesive systems containing Bioglass and two modified Portland cement-based micro- fillers were found to reduce nano-leakage with no negative effects on bond strength. Furthermore, specimens created with the same experimental adhesives did not restore micro-hardness to the level of sound dentine but were able to maintain statistically unaltered Knoop values.
The second section is also composed of a set of preliminary studies that involved the use of up-to-date spectroscopic (attenuated total reflection Fourier transform infrared spectroscopy) and thermoanalytical (differential scanning calorimetry) techniques to predict the chemical-physical properties and apatite- forming ability of the novel ion-leachable hybrid materials. Lastly, the overall conclusions of the present work and directions for future research are discussed.
"]},{"key":"dc:title","label":"Title","values":["Hybridisation of dental hard tissues with modified adhesive systems: therapeutic impact of bioactive silicate compounds on bonding to dentine"]}]}],"canonical_facts":{"dc:contributor.advisor":["Foxton, Richard Mark"],"dc:creator":["Corrado Profeta, Andrea"],"dc:date":["2013-7-1"],"dc:date.issued":["2013-7-1"],"dc:description.abstract":["The first section of this work is a review of the literature necessary to understand the objectives of the project; it includes general information about dental adhesive technology as well as adhesion testing, about dentine hybridisation and about the drawbacks of contemporary bonding systems. Several studies revealed excellent immediate and short-term bonding effectiveness of etch-and-rinse adhesives, yet substantial reductions in resin- dentine bond strength occur after ageing. Degenerative phenomena involve hydrolysis of suboptimally polymerised hydrophilic resin components and degradation of mineral-deprived water-rich resin-sparse collagen matrices by matrix metalloproteinases and cysteine cathepsins.
Silicate compounds, including calcium/sodium phosphosilicates, such as commercially available bioactive glass, and calcium-silicate Portland-derived cements are known to promote the formation of apatite in aqueous environments that contain calcium and phosphate (e.g. saliva); thus, we have raised questions about whether their presence at the bonded interface could increase the in vitro durability of resin-dentine bonds through crystal formation and self-sealing, in the presence of phosphate buffered saline or simulated body fluid solutions.
In answering these questions, the objectives were accomplished by employing Bioglass® 45S5 in etch-and-rinse bonding procedures either (i) included within the composition of a resin adhesive as a tailored micro-filler, or (ii) applied directly onto acid-etched wetted dentine. Alternative light-curable methacrylate-based agents containing (iii) three modified calcium-silicates derived from ordinary Portland cement were also tested.
Confirming the relative success of bioactive materials incorporated in the dentine bonding procedures required assessment of the potential to reduce nano-leakage, as well as their effect upon the strength of the bond over time.
In order to explore these possibilities, which have not been previously investigated, a combination of methods were applied in the second experimental section. Bond strength variations were quantified using the microtensile test while scanning electron microscopy, confocal laser scanning microscopy and Knoop micro-indentation analysis were used to evaluate optically and mechanically adjustments to mineral and water content within the resin bonded-dentine interface. Initially, high microtensile values were achieved in each tested group. All the resin-dentine interfaces created with bonding agents containing micro-fillers showed an evident reduction of nano-leakage and mineral deposition after the ageing period. However, only adhesive systems containing Bioglass and two modified Portland cement-based micro- fillers were found to reduce nano-leakage with no negative effects on bond strength. Furthermore, specimens created with the same experimental adhesives did not restore micro-hardness to the level of sound dentine but were able to maintain statistically unaltered Knoop values.
The second section is also composed of a set of preliminary studies that involved the use of up-to-date spectroscopic (attenuated total reflection Fourier transform infrared spectroscopy) and thermoanalytical (differential scanning calorimetry) techniques to predict the chemical-physical properties and apatite- forming ability of the novel ion-leachable hybrid materials. Lastly, the overall conclusions of the present work and directions for future research are discussed.
"],"dc:identifier":["oai:kclpure.kcl.ac.uk:studenttheses/46cfba10-5ebb-41a2-9db3-1892daa17e76","https://kclpure.kcl.ac.uk/portal/en/studentTheses/46cfba10-5ebb-41a2-9db3-1892daa17e76"],"dc:identifier.uri":["https://kclpure.kcl.ac.uk/portal/files/11772676/Studentthesis-Andrea_Corrado%20Profeta_2013.pdf"],"dc:language":["eng"],"dc:publisher.department":["Conservative Dentistry (including Endodontics)"],"dc:publisher.institution":["King's College London"],"dc:relation.isreferencedby":["https://kclpure.kcl.ac.uk/portal/en/studentTheses/46cfba10-5ebb-41a2-9db3-1892daa17e76"],"dc:title":["Hybridisation of dental hard tissues with modified adhesive systems: therapeutic impact of bioactive silicate compounds on bonding to dentine"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral Thesis"],"dc:type.qualificationname":["Doctor of Philosophy"]},"updated_at":"2026-07-24T02:44:31Z"}