{"id":{"repo_id":"iupui","oai_identifier":"oai:scholarworks.indianapolis.iu.edu:1805/6229"},"canonical_url":"https://search.dev.ndltd.org/etd/iupui/oai:scholarworks.indianapolis.iu.edu:1805/6229","repository":{"repo_id":"iupui","name":"IUPUI","base_url":"https://scholarworks.indianapolis.iu.edu/server/oai/request"},"display":{"title":"Nmp4 restricts bone marrow osteoprogenitors and parathyroid hormone induced bone formation in healthy and estrogen depleted female mice","abstract":"We have shown that nuclear matrix protein 4 (Nmp4) attenuates the response to intermittent parathyroid hormone (PTH) in healthy and ovariectomized (OVX) female mice using a global knockout of the Nmp4 gene. Additionally, these mice have increased bone marrow osteoprogenitors and CD8+ T-cells which support osteoblast differentiation. The animals were not protected from bone loss following OVX, but retained the hypersensitivity seen in the intact mice. Mesenchymal stem/progenitor cells (osteoprogenitors) demonstrated increased growth rate in culture and showed more robust differentiation into mineralizing bone cells. Chromosome precipitation followed by next generation sequencing and bioinformatics analysis characterized Nmp4 as a negative regulator of synthetic processes and suggested the IGF1/Akt and BMP2/Smad biochemical pathways which are likely targets for Nmp4 regulation. We have experimentally verified these pathways in immortalized bone marrow mesenchymal cells from wild type and Nmp4-KO mice. Disabling Nmp4 in estrogen replete or depleted mice confers an enhanced bone formation from intermittent parathyroid hormone.","abstract_html":"We have shown that nuclear matrix protein 4 (Nmp4) attenuates the response to intermittent parathyroid hormone (PTH) in healthy and ovariectomized (OVX) female mice using a global knockout of the Nmp4 gene. Additionally, these mice have increased bone marrow osteoprogenitors and CD8+ T-cells which support osteoblast differentiation. The animals were not protected from bone loss following OVX, but retained the hypersensitivity seen in the intact mice. Mesenchymal stem/progenitor cells (osteoprogenitors) demonstrated increased growth rate in culture and showed more robust differentiation into mineralizing bone cells. Chromosome precipitation followed by next generation sequencing and bioinformatics analysis characterized Nmp4 as a negative regulator of synthetic processes and suggested the IGF1/Akt and BMP2/Smad biochemical pathways which are likely targets for Nmp4 regulation. We have experimentally verified these pathways in immortalized bone marrow mesenchymal cells from wild type and Nmp4-KO mice. Disabling Nmp4 in estrogen replete or depleted mice confers an enhanced bone formation from intermittent parathyroid hormone.","abstract_has_math":false,"creators":["Childress, Paul Jeffrey"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Bidwell, Joseph P."],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12","date_published":"2014-12","updated_at":"2026-07-24T02:40:35Z","subjects":["Nmp4","Parathyroid hormone","Osteoprogenitor","Estrogen depletion"],"languages":["en_US"],"rights":["CC0 1.0 Universal"],"rights_urls":["http://creativecommons.org/publicdomain/zero/1.0/"],"identifier_entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://dx.doi.org/10.7912/C2/2101"],"render_values":[{"text":"http://dx.doi.org/10.7912/C2/2101","href":"http://dx.doi.org/10.7912/C2/2101","code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/1805/6229","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Bidwell, Joseph P."]},{"key":"dc:contributor.other","label":"Dc Contributor Other","values":["Pavalko, Fredrick M.","Bellido, Teresita","Robling, Alexander G.","Kacena, Melissa A."]},{"key":"dc:creator","label":"Author","values":["Childress, Paul Jeffrey"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-04-22T19:22:36Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-04-22T19:22:36Z"]},{"key":"dc:date.issued","label":"Date","values":["2014-12"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nmp4","Parathyroid hormone","Osteoprogenitor","Estrogen depletion"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["CC0 1.0 Universal"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://creativecommons.org/publicdomain/zero/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1805/6229","http://dx.doi.org/10.7912/C2/2101"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["We have shown that nuclear matrix protein 4 (Nmp4) attenuates the response to intermittent parathyroid hormone (PTH) in healthy and ovariectomized (OVX) female mice using a global knockout of the Nmp4 gene. Additionally, these mice have increased bone marrow osteoprogenitors and CD8+ T-cells which support osteoblast differentiation. The animals were not protected from bone loss following OVX, but retained the hypersensitivity seen in the intact mice. Mesenchymal stem/progenitor cells (osteoprogenitors) demonstrated increased growth rate in culture and showed more robust differentiation into mineralizing bone cells. Chromosome precipitation followed by next generation sequencing and bioinformatics analysis characterized Nmp4 as a negative regulator of synthetic processes and suggested the IGF1/Akt and BMP2/Smad biochemical pathways which are likely targets for Nmp4 regulation. We have experimentally verified these pathways in immortalized bone marrow mesenchymal cells from wild type and Nmp4-KO mice. Disabling Nmp4 in estrogen replete or depleted mice confers an enhanced bone formation from intermittent parathyroid hormone."]},{"key":"dc:title","label":"Title","values":["Nmp4 restricts bone marrow osteoprogenitors and parathyroid hormone induced bone formation in healthy and estrogen depleted female mice"]}]}],"canonical_facts":{"dc:contributor.advisor":["Bidwell, Joseph P."],"dc:contributor.other":["Pavalko, Fredrick M.","Bellido, Teresita","Robling, Alexander G.","Kacena, Melissa A."],"dc:creator":["Childress, Paul Jeffrey"],"dc:date.accessioned":["2015-04-22T19:22:36Z"],"dc:date.available":["2015-04-22T19:22:36Z"],"dc:date.issued":["2014-12"],"dc:description.abstract":["We have shown that nuclear matrix protein 4 (Nmp4) attenuates the response to intermittent parathyroid hormone (PTH) in healthy and ovariectomized (OVX) female mice using a global knockout of the Nmp4 gene. Additionally, these mice have increased bone marrow osteoprogenitors and CD8+ T-cells which support osteoblast differentiation. The animals were not protected from bone loss following OVX, but retained the hypersensitivity seen in the intact mice. Mesenchymal stem/progenitor cells (osteoprogenitors) demonstrated increased growth rate in culture and showed more robust differentiation into mineralizing bone cells. Chromosome precipitation followed by next generation sequencing and bioinformatics analysis characterized Nmp4 as a negative regulator of synthetic processes and suggested the IGF1/Akt and BMP2/Smad biochemical pathways which are likely targets for Nmp4 regulation. We have experimentally verified these pathways in immortalized bone marrow mesenchymal cells from wild type and Nmp4-KO mice. Disabling Nmp4 in estrogen replete or depleted mice confers an enhanced bone formation from intermittent parathyroid hormone."],"dc:identifier.uri":["https://hdl.handle.net/1805/6229","http://dx.doi.org/10.7912/C2/2101"],"dc:language.iso":["en_US"],"dc:rights":["CC0 1.0 Universal"],"dc:rights.uri":["http://creativecommons.org/publicdomain/zero/1.0/"],"dc:subject":["Nmp4","Parathyroid hormone","Osteoprogenitor","Estrogen depletion"],"dc:title":["Nmp4 restricts bone marrow osteoprogenitors and parathyroid hormone induced bone formation in healthy and estrogen depleted female mice"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T02:40:35Z"}