Effects of aflatoxin on cell-mediated immunity were investigated in guinea pigs. Dose dependent reductions in skin test responses were observed in guinea pigs which received at least 0.040 mg/kg/day aflatoxin B(,1) equivalents (B(,1) eq) for 3 weeks. Reduced weight gains were apparent at the 0.020 mg/kg/day B(,1) eq level. Serum bile acids were elevated at low levels of aflatoxin ingestion, in guinea pigs which displayed normal skin test responsiveness and weight gain;Passive transfer of delayed type hypersensitivity (DTH) was used to evaluate T lymphocytes or macrophages from aflatoxin-treated guinea pigs. The skin test responses of both nontreated guinea pigs receiving peritoneal exudate cells (PEC) from aflatoxin-treated guinea pigs and aflatoxin-treated guinea pigs receiving PEC from nontreated guinea pigs were numerically lower, but not significantly different (P < 0.05), than that of controls. Variability in susceptibility to aflatoxin was noted among individuals within groups;A bacterial binding assay currently used to identify human lymphocytes was adapted for use in guinea pigs. Three lymphocyte markers were identified: Salmonella schottmulleri and Yersinia enterocolitica, which appear to label all or some guinea pig T lymphocytes in peripheral blood; and Brucella melitensis, identified as a marker for guinea pig B lymphocytes in peripheral blood. Lymphocytes from spleen, thymus, and lymph node had different binding capacities than lymphocytes in peripheral blood, reflecting perhaps maturational differences among lymphocytes from different sources;Lymphocytes from nontreated and aflatoxin-treated guinea pigs were counted using immunofluorescence and bacterial markers. No changes occurred in absolute number of B or T lymphocytes identified by immunofluorescence. The number of S schottmulleri-positive lymphocytes closely approximated the numbr of T lymphocytes identified by immunofluorescence, except at the highest dose of aflatoxin used. At this level (0.060 mg/kg/day B(,1) eq) some T lymphocytes identified by immunofluorescence were refractory to binding of S schottmulleri;Aflatoxin impaired skin test responsiveness and passive transfer of DTH without causing quantitative changes in T or B lymphocyte populations in peripheral blood.
","abstract_html":"<p>Effects of aflatoxin on cell-mediated immunity were investigated in guinea pigs. Dose dependent reductions in skin test responses were observed in guinea pigs which received at least 0.040 mg/kg/day aflatoxin B(,1) equivalents (B(,1) eq) for 3 weeks. Reduced weight gains were apparent at the 0.020 mg/kg/day B(,1) eq level. Serum bile acids were elevated at low levels of aflatoxin ingestion, in guinea pigs which displayed normal skin test responsiveness and weight gain;Passive transfer of delayed type hypersensitivity (DTH) was used to evaluate T lymphocytes or macrophages from aflatoxin-treated guinea pigs. The skin test responses of both nontreated guinea pigs receiving peritoneal exudate cells (PEC) from aflatoxin-treated guinea pigs and aflatoxin-treated guinea pigs receiving PEC from nontreated guinea pigs were numerically lower, but not significantly different (P < 0.05), than that of controls. Variability in susceptibility to aflatoxin was noted among individuals within groups;A bacterial binding assay currently used to identify human lymphocytes was adapted for use in guinea pigs. Three lymphocyte markers were identified: Salmonella schottmulleri and Yersinia enterocolitica, which appear to label all or some guinea pig T lymphocytes in peripheral blood; and Brucella melitensis, identified as a marker for guinea pig B lymphocytes in peripheral blood. Lymphocytes from spleen, thymus, and lymph node had different binding capacities than lymphocytes in peripheral blood, reflecting perhaps maturational differences among lymphocytes from different sources;Lymphocytes from nontreated and aflatoxin-treated guinea pigs were counted using immunofluorescence and bacterial markers. No changes occurred in absolute number of B or T lymphocytes identified by immunofluorescence. The number of S schottmulleri-positive lymphocytes closely approximated the numbr of T lymphocytes identified by immunofluorescence, except at the highest dose of aflatoxin used. At this level (0.060 mg/kg/day B(,1) eq) some T lymphocytes identified by immunofluorescence were refractory to binding of S schottmulleri;Aflatoxin impaired skin test responsiveness and passive transfer of DTH without causing quantitative changes in T or B lymphocyte populations in peripheral blood.</p>","abstract_has_math":false,"creators":["McLoughlin, Mary"],"institution":null,"degree_name":"Doctor of Philosophy","degree_level":"dissertation","degree_discipline":"Immunobiology","degree_department":"Theses & dissertations (Interdisciplinary)","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1982,"date_issued":"1982","date_published":"1982","updated_at":"2026-07-24T02:38:46Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.31274/rtd-180813-1801"],"render_values":[{"text":"https://doi.org/10.31274/rtd-180813-1801","href":"https://doi.org/10.31274/rtd-180813-1801","code":true}]},{"key":"dc:identifier","label":"Identifier","values":["archive/lib.dr.iastate.edu/rtd/8369/"],"render_values":[{"text":"archive/lib.dr.iastate.edu/rtd/8369/","href":null,"code":true}]}]},"links":{"outbound_url":"https://dr.lib.iastate.edu/handle/20.500.12876/81349","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Theses & dissertations (Interdisciplinary)"]},{"key":"dc:creator","label":"Author","values":["McLoughlin, Mary"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-08-15T22:45:57.000"]},{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2020-07-02T06:06:26Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2020-07-02T06:06:26Z"]},{"key":"dc:date.issued","label":"Date","values":["1982"]},{"key":"dc:type","label":"Dc Type","values":["dissertation"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Immunobiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["archive/lib.dr.iastate.edu/rtd/8369/"]},{"key":"dc:identifier.doi","label":"DOI","values":["https://doi.org/10.31274/rtd-180813-1801"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://dr.lib.iastate.edu/handle/20.500.12876/81349"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Effects of aflatoxin on cell-mediated immunity were investigated in guinea pigs. Dose dependent reductions in skin test responses were observed in guinea pigs which received at least 0.040 mg/kg/day aflatoxin B(,1) equivalents (B(,1) eq) for 3 weeks. Reduced weight gains were apparent at the 0.020 mg/kg/day B(,1) eq level. Serum bile acids were elevated at low levels of aflatoxin ingestion, in guinea pigs which displayed normal skin test responsiveness and weight gain;Passive transfer of delayed type hypersensitivity (DTH) was used to evaluate T lymphocytes or macrophages from aflatoxin-treated guinea pigs. The skin test responses of both nontreated guinea pigs receiving peritoneal exudate cells (PEC) from aflatoxin-treated guinea pigs and aflatoxin-treated guinea pigs receiving PEC from nontreated guinea pigs were numerically lower, but not significantly different (P < 0.05), than that of controls. Variability in susceptibility to aflatoxin was noted among individuals within groups;A bacterial binding assay currently used to identify human lymphocytes was adapted for use in guinea pigs. Three lymphocyte markers were identified: Salmonella schottmulleri and Yersinia enterocolitica, which appear to label all or some guinea pig T lymphocytes in peripheral blood; and Brucella melitensis, identified as a marker for guinea pig B lymphocytes in peripheral blood. Lymphocytes from spleen, thymus, and lymph node had different binding capacities than lymphocytes in peripheral blood, reflecting perhaps maturational differences among lymphocytes from different sources;Lymphocytes from nontreated and aflatoxin-treated guinea pigs were counted using immunofluorescence and bacterial markers. No changes occurred in absolute number of B or T lymphocytes identified by immunofluorescence. The number of S schottmulleri-positive lymphocytes closely approximated the numbr of T lymphocytes identified by immunofluorescence, except at the highest dose of aflatoxin used. At this level (0.060 mg/kg/day B(,1) eq) some T lymphocytes identified by immunofluorescence were refractory to binding of S schottmulleri;Aflatoxin impaired skin test responsiveness and passive transfer of DTH without causing quantitative changes in T or B lymphocyte populations in peripheral blood.
"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Lymphocyte populations and their relationship to cell-mediated immune responses in aflatoxin-treated guinea pigs"]}]}],"canonical_facts":{"dc:contributor.department":["Theses & dissertations (Interdisciplinary)"],"dc:creator":["McLoughlin, Mary"],"dc:date":["2018-08-15T22:45:57.000"],"dc:date.accessioned":["2020-07-02T06:06:26Z"],"dc:date.available":["2020-07-02T06:06:26Z"],"dc:date.issued":["1982"],"dc:description.abstract":["Effects of aflatoxin on cell-mediated immunity were investigated in guinea pigs. Dose dependent reductions in skin test responses were observed in guinea pigs which received at least 0.040 mg/kg/day aflatoxin B(,1) equivalents (B(,1) eq) for 3 weeks. Reduced weight gains were apparent at the 0.020 mg/kg/day B(,1) eq level. Serum bile acids were elevated at low levels of aflatoxin ingestion, in guinea pigs which displayed normal skin test responsiveness and weight gain;Passive transfer of delayed type hypersensitivity (DTH) was used to evaluate T lymphocytes or macrophages from aflatoxin-treated guinea pigs. The skin test responses of both nontreated guinea pigs receiving peritoneal exudate cells (PEC) from aflatoxin-treated guinea pigs and aflatoxin-treated guinea pigs receiving PEC from nontreated guinea pigs were numerically lower, but not significantly different (P < 0.05), than that of controls. Variability in susceptibility to aflatoxin was noted among individuals within groups;A bacterial binding assay currently used to identify human lymphocytes was adapted for use in guinea pigs. Three lymphocyte markers were identified: Salmonella schottmulleri and Yersinia enterocolitica, which appear to label all or some guinea pig T lymphocytes in peripheral blood; and Brucella melitensis, identified as a marker for guinea pig B lymphocytes in peripheral blood. Lymphocytes from spleen, thymus, and lymph node had different binding capacities than lymphocytes in peripheral blood, reflecting perhaps maturational differences among lymphocytes from different sources;Lymphocytes from nontreated and aflatoxin-treated guinea pigs were counted using immunofluorescence and bacterial markers. No changes occurred in absolute number of B or T lymphocytes identified by immunofluorescence. The number of S schottmulleri-positive lymphocytes closely approximated the numbr of T lymphocytes identified by immunofluorescence, except at the highest dose of aflatoxin used. At this level (0.060 mg/kg/day B(,1) eq) some T lymphocytes identified by immunofluorescence were refractory to binding of S schottmulleri;Aflatoxin impaired skin test responsiveness and passive transfer of DTH without causing quantitative changes in T or B lymphocyte populations in peripheral blood.
"],"dc:format.mimetype":["application/pdf"],"dc:identifier":["archive/lib.dr.iastate.edu/rtd/8369/"],"dc:identifier.doi":["https://doi.org/10.31274/rtd-180813-1801"],"dc:identifier.uri":["https://dr.lib.iastate.edu/handle/20.500.12876/81349"],"dc:language.iso":["en"],"dc:title":["Lymphocyte populations and their relationship to cell-mediated immune responses in aflatoxin-treated guinea pigs"],"dc:type":["dissertation"],"thesis:degree_discipline":["Immunobiology"],"thesis:degree_level":["dissertation"],"thesis:degree_name":["Doctor of Philosophy"]},"updated_at":"2026-07-24T02:38:46Z"}