Iowa State University
The Fourier convolution-deconvolution method: its limitation and application
Abstract
dc:description.abstract<p>The ability to measure distances within a biomolecule is an important tool for determining structure and function. The electron paramagnetic resonance (EPR) Fourier Convolution-Deconvolution method of measuring distance was utilized to determine the distance between two cysteine mutated residues in the linker region of Sso1p in order to determine the conformation of this region during SNARE complex assembly. A 12 -14 Angstrom distance measurement suggests this region to alpha helical, with a small unstructured area, during complex formation. In addition the limits of distance measurements were tested using the continuous wave electron paramagnetic resonance (cwEPR) method of Fourier Convolution-Deconvolution. This method was unable to measure a known 32 Angstrom distance within the C-terminus region of the SNAP-25 protein.</p>
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- thesis
- Discipline thesis:degree_discipline
- Chemistry
- Department dc:contributor.department
- Department of Chemistry
- Year dc:date.issued
- 2006
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Gugliotta, Nicole
Rights
- Language dc:language.iso
- en
Identifiers
dc:identifier.*- Identifier
- archive/lib.dr.iastate.edu/rtd/19409/
- OAI identifier oai:identifier
- oai:dr.lib.iastate.edu:20.500.12876/73416