Carbon-13 metabolic flux analysis of soybean central carbon metabolism: response to temperature and genotype effects
type: CreativeWork
description: Soybeans are a major source of high quality protein, edible oil, food products, and also several industrial non food applications such as paper coating, textiles and plastics. It is difficult to develop soybeans with high protein and high oil, however, due to the inverse relationship between these two components. Consequently, increasing seed protein concentration without adversely affecting the yield and oil content has been difficult to achieve. It has been demonstrated that the temperature and genotype of soybeans influence the protein or lipid composition considerably. However, the interactions between various pathways responsible for the changes in the composition are unknown;Metabolic flux analysis (MFA) quantifies carbon flow in a biological system, which is an important characteristic reflective of the system physiology. The application of MFA to study complex plant metabolic networks has however been recent. Towards this goal, MFA using carbon labeling (13C) experiments, nuclear magnetic resonance spectroscopy (NMR) combined with a generic mathematical framework (NMR2Flux) has been developed in our group. Metabolic flux maps, developed from 13C MFA are effective tools for comparing pathway interactions between genetic or environmental variants of biological systems and identifying possible targets for genetic manipulations;13C MFA has been performed to understand response of central carbon metabolism of developing soybeans to two parameters: temperature and genotype. Experimental treatments were designed to distinguish between temperature effects prior to and during incubation in vitro of Evans genotype. Biomass accumulation increased with temperature as did carbon partitioning into lipid. The flux through the plastidic oxidative pentose phosphate pathway ( pglP) relative to total sucrose intake remained fairly constant (~56 % (+/-24%)) when cotyledons were transferred from an optimum growth temperature in planta to optimum, lower and higher temperatures in…
abstract: Soybeans are a major source of high quality protein, edible oil, food products, and also several industrial non food applications such as paper coating, textiles and plastics. It is difficult to develop soybeans with high protein and high oil, however, due to the inverse relationship between these two components. Consequently, increasing seed protein concentration without adversely affecting the yield and oil content has been difficult to achieve. It has been demonstrated that the temperature and genotype of soybeans influence the protein or lipid composition considerably. However, the interactions between various pathways responsible for the changes in the composition are unknown;Metabolic flux analysis (MFA) quantifies carbon flow in a biological system, which is an important characteristic reflective of the system physiology. The application of MFA to study complex plant metabolic networks has however been recent. Towards this goal, MFA using carbon labeling (13C) experiments, nuclear magnetic resonance spectroscopy (NMR) combined with a generic mathematical framework (NMR2Flux) has been developed in our group. Metabolic flux maps, developed from 13C MFA are effective tools for comparing pathway interactions between genetic or environmental variants of biological systems and identifying possible targets for genetic manipulations;13C MFA has been performed to understand response of central carbon metabolism of developing soybeans to two parameters: temperature and genotype. Experimental treatments were designed to distinguish between temperature effects prior to and during incubation in vitro of Evans genotype. Biomass accumulation increased with temperature as did carbon partitioning into lipid. The flux through the plastidic oxidative pentose phosphate pathway ( pglP) relative to total sucrose intake remained fairly constant (~56 % (+/-24%)) when cotyledons were transferred from an optimum growth temperature in planta to optimum, lower and higher temperatures in…
author: Iyer, Vidya
language: en
date published: 2006-01-01
date modified: 2026-07-24T02:39:07Z
provider: Iowa State University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Iowa State University

PropertyValue
property ID: DOI
value: 10.31274/rtd-180813-11945