https://search.dev.ndltd.org/etd/iastate/oai:dr.lib.iastate.edu:20.500.12876/30212
type: CreativeWork
name: Comparison of the Kinetics and Spectral Properties of AuCl4- Binding by Methanobactins from Methylosinus trichosporium OB3b and Methylocystis Strain SB2: Evidence of Exciton Disruption between Intramolecular Chromophores
description: Methanobactin (Mb) is the first characterized example of a chalkophore or copper binding protein. Mbs are produced by most aerobic methane oxidizing bacteria for Cu recruitment to the cell and eventually incorporation into the central metabolism. In addition to the biological purpose of Cu binding, mbs bind a number of transition and near transition metals. Within this text, the metal binding properties are explored and compared between two mbs, which represent two distinct groups of mbs, mb from Methylosinus trichosporium OB3b and mb from Methylocystis strain SB2. The Cu binding properties of these mbs have been previously explored, however, herein, the binding and displacement properties of each mb are presented for a number of transition metals. The binding properties of the metals able to displace Cu from Cu bound mb (Cu-mb) are of particular interest due to the extremely high affinity with which mbs bind Cu. Mercury is one such example, and the binding properties of Hg, in the forms Hg2+, Hg(CN)2, and CH3Hg+, are examined for mb from Methylocystis strain SB2. Each form was bound slightly differently by mb-SB2. Chapter 2 presents the characterization of the Hg binding, in each form, for mb-SB2. Au also displaces Cu-mb for both mb-OB3b and SB2. In the final chapter, the Au binding properties of mb-OB3b and mb-SB2 are compared. Previously collected CD spectra of mb-SB2 titrated with Au(III) suggested that an exciton transfer exists between the chromophores of mb-SB2. Herein, stopped flow UV-vis kinetic traces of mb-OB3b titrated with Au(III) demonstrate that an exciton transfer is present between the chromophores of mb-OB3b.
abstract: Methanobactin (Mb) is the first characterized example of a chalkophore or copper binding protein. Mbs are produced by most aerobic methane oxidizing bacteria for Cu recruitment to the cell and eventually incorporation into the central metabolism. In addition to the biological purpose of Cu binding, mbs bind a number of transition and near transition metals. Within this text, the metal binding properties are explored and compared between two mbs, which represent two distinct groups of mbs, mb from Methylosinus trichosporium OB3b and mb from Methylocystis strain SB2. The Cu binding properties of these mbs have been previously explored, however, herein, the binding and displacement properties of each mb are presented for a number of transition metals. The binding properties of the metals able to displace Cu from Cu bound mb (Cu-mb) are of particular interest due to the extremely high affinity with which mbs bind Cu. Mercury is one such example, and the binding properties of Hg, in the forms Hg2+, Hg(CN)2, and CH3Hg+, are examined for mb from Methylocystis strain SB2. Each form was bound slightly differently by mb-SB2. Chapter 2 presents the characterization of the Hg binding, in each form, for mb-SB2. Au also displaces Cu-mb for both mb-OB3b and SB2. In the final chapter, the Au binding properties of mb-OB3b and mb-SB2 are compared. Previously collected CD spectra of mb-SB2 titrated with Au(III) suggested that an exciton transfer exists between the chromophores of mb-SB2. Herein, stopped flow UV-vis kinetic traces of mb-OB3b titrated with Au(III) demonstrate that an exciton transfer is present between the chromophores of mb-OB3b.
author: Turpin, Erick
language: en
date published: 2016-01-01
date modified: 2026-07-24T02:37:38Z
provider: Iowa State University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Iowa State University

PropertyValue
property ID: DOI
value: 10.31274/etd-180810-5656