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The gel supported system was designed to provide greater stability to the reagents during the analysis process and also during long periods of dormancy, enabling the mass production of one use micro-fluidic device, encapsulating all required reagents at time of manufacturing.Described is the application of electro-osmotic pumping through a gel supported reagent matrix, where a silica monolith was used to support both the electro-osmotic pumping mechanism and the extraction of DNA from cellular debris. The gel supported system also enabled the delivery of a precise and accurate sample plug by an electro-kinetic pinched injection across a gel-to-gel interface, contributing to the improvement and optimisation of the separation of the DNA by capillary electrophoresis. 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