{"id":{"repo_id":"houston","oai_identifier":"oai:uh-ir.tdl.org:10657/689"},"canonical_url":"https://search.dev.ndltd.org/etd/houston/oai:uh-ir.tdl.org:10657/689","repository":{"repo_id":"houston","name":"University of Houston","base_url":"https://uh-ir.tdl.org/server/oai/request"},"display":{"title":"Synthesis of Inhibitors of Cystathionine β-Synthase","abstract":"Hydrogen sulfide (H2S) is well known for its harmful effects to the environment, yet within the last decade evidence of its role as an endogenous gasotransmitter has surfaced. Both overproduction and underproduction of H2S can lead to detrimental health conditions; therefore, adequate metabolism of H2S is required to maintain homeostasis. Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) are the two main PLP-dependent enzymes that contribute to H2S metabolism through generation from L-cysteine. A recent study has discovered a link between CBS-derived H2S and tumor growth in colorectal cancer. Overproduction of H2S in colon cancer cells leads to an increase in ATP generation and development of new blood vessels, which are two factors required for tumor growth. Therefore, it is promising to develop potent and selective inhibitors of the CBS enzyme to serve as potential drug therapies for colorectal cancer. Aminooxyacetic acid (AOA) is an inhibitor of CBS, yet has a major limitation in that it is not selective and inhibits a variety of other PLP-dependent enzymes. To overcome this limitation, two AOA conjugates were synthesized in an attempt to deliver the inhibitor into the mitochondria. The use of the triphenylphosphonium cation should allow for the selective accumulation of AOA into the mitochondria due to the high inner membrane potential gradient. Modification of the carboxylic acid functionality of AOA to an ester and amide functionality will also allow for comparison to AOA inhibitor activity once tested in animals. Another modification of AOA was made by converting the oxyamino functionality into a hydroxylamino functionality, in an effort to enhance the bioactivity and drug-properties of the compound. In addition, the CBS product, L, L-cystathionine, was modified by converting the amino group of the serine (or cysteine) derived half of the product into a carbonyl group, although purification was not successful. Both of the aforementioned modifications will allow for comparison to natural inhibitor and product binding activities, respectively.","abstract_html":"Hydrogen sulfide (H2S) is well known for its harmful effects to the environment, yet within the last decade evidence of its role as an endogenous gasotransmitter has surfaced. Both overproduction and underproduction of H2S can lead to detrimental health conditions; therefore, adequate metabolism of H2S is required to maintain homeostasis. Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) are the two main PLP-dependent enzymes that contribute to H2S metabolism through generation from L-cysteine. A recent study has discovered a link between CBS-derived H2S and tumor growth in colorectal cancer. Overproduction of H2S in colon cancer cells leads to an increase in ATP generation and development of new blood vessels, which are two factors required for tumor growth. Therefore, it is promising to develop potent and selective inhibitors of the CBS enzyme to serve as potential drug therapies for colorectal cancer. Aminooxyacetic acid (AOA) is an inhibitor of CBS, yet has a major limitation in that it is not selective and inhibits a variety of other PLP-dependent enzymes. To overcome this limitation, two AOA conjugates were synthesized in an attempt to deliver the inhibitor into the mitochondria. The use of the triphenylphosphonium cation should allow for the selective accumulation of AOA into the mitochondria due to the high inner membrane potential gradient. Modification of the carboxylic acid functionality of AOA to an ester and amide functionality will also allow for comparison to AOA inhibitor activity once tested in animals. Another modification of AOA was made by converting the oxyamino functionality into a hydroxylamino functionality, in an effort to enhance the bioactivity and drug-properties of the compound. In addition, the CBS product, L, L-cystathionine, was modified by converting the amino group of the serine (or cysteine) derived half of the product into a carbonyl group, although purification was not successful. Both of the aforementioned modifications will allow for comparison to natural inhibitor and product binding activities, respectively.","abstract_has_math":false,"creators":["Guajardo, Alyssa Michelle 1989-"],"institution":"University of Houston","degree_name":"Master of Science","degree_level":"Masters","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":["Gilbertson, Scott R."],"committee_chairs":[],"committee_members":["Bark, Steven J.","Coltart, Don M.","Xu, Shoujun"],"year":2014,"date_issued":"2014-05","date_published":"2014-05","updated_at":"2026-07-24T02:32:34Z","subjects":["Cystathionine β-synthase","Colorectal cancer","Aminooxyacetic acid"],"languages":["eng"],"rights":["The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s)."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10657/689","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Gilbertson, Scott R."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Bark, Steven J.","Coltart, Don M.","Xu, Shoujun"]},{"key":"dc:creator","label":"Author","values":["Guajardo, Alyssa Michelle 1989-"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-07-21T15:53:58Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-21T15:53:58Z"]},{"key":"dc:date.issued","label":"Date","values":["2014-05"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Houston"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cystathionine β-synthase","Colorectal cancer","Aminooxyacetic acid"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s)."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10657/689"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Hydrogen sulfide (H2S) is well known for its harmful effects to the environment, yet within the last decade evidence of its role as an endogenous gasotransmitter has surfaced. Both overproduction and underproduction of H2S can lead to detrimental health conditions; therefore, adequate metabolism of H2S is required to maintain homeostasis. Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) are the two main PLP-dependent enzymes that contribute to H2S metabolism through generation from L-cysteine. A recent study has discovered a link between CBS-derived H2S and tumor growth in colorectal cancer. Overproduction of H2S in colon cancer cells leads to an increase in ATP generation and development of new blood vessels, which are two factors required for tumor growth. Therefore, it is promising to develop potent and selective inhibitors of the CBS enzyme to serve as potential drug therapies for colorectal cancer. Aminooxyacetic acid (AOA) is an inhibitor of CBS, yet has a major limitation in that it is not selective and inhibits a variety of other PLP-dependent enzymes. To overcome this limitation, two AOA conjugates were synthesized in an attempt to deliver the inhibitor into the mitochondria. The use of the triphenylphosphonium cation should allow for the selective accumulation of AOA into the mitochondria due to the high inner membrane potential gradient. Modification of the carboxylic acid functionality of AOA to an ester and amide functionality will also allow for comparison to AOA inhibitor activity once tested in animals. Another modification of AOA was made by converting the oxyamino functionality into a hydroxylamino functionality, in an effort to enhance the bioactivity and drug-properties of the compound. In addition, the CBS product, L, L-cystathionine, was modified by converting the amino group of the serine (or cysteine) derived half of the product into a carbonyl group, although purification was not successful. Both of the aforementioned modifications will allow for comparison to natural inhibitor and product binding activities, respectively."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Synthesis of Inhibitors of Cystathionine β-Synthase"]}]}],"canonical_facts":{"dc:contributor.advisor":["Gilbertson, Scott R."],"dc:contributor.committeemember":["Bark, Steven J.","Coltart, Don M.","Xu, Shoujun"],"dc:creator":["Guajardo, Alyssa Michelle 1989-"],"dc:date.accessioned":["2014-07-21T15:53:58Z"],"dc:date.available":["2014-07-21T15:53:58Z"],"dc:date.issued":["2014-05"],"dc:description.abstract":["Hydrogen sulfide (H2S) is well known for its harmful effects to the environment, yet within the last decade evidence of its role as an endogenous gasotransmitter has surfaced. Both overproduction and underproduction of H2S can lead to detrimental health conditions; therefore, adequate metabolism of H2S is required to maintain homeostasis. Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) are the two main PLP-dependent enzymes that contribute to H2S metabolism through generation from L-cysteine. A recent study has discovered a link between CBS-derived H2S and tumor growth in colorectal cancer. Overproduction of H2S in colon cancer cells leads to an increase in ATP generation and development of new blood vessels, which are two factors required for tumor growth. Therefore, it is promising to develop potent and selective inhibitors of the CBS enzyme to serve as potential drug therapies for colorectal cancer. Aminooxyacetic acid (AOA) is an inhibitor of CBS, yet has a major limitation in that it is not selective and inhibits a variety of other PLP-dependent enzymes. To overcome this limitation, two AOA conjugates were synthesized in an attempt to deliver the inhibitor into the mitochondria. The use of the triphenylphosphonium cation should allow for the selective accumulation of AOA into the mitochondria due to the high inner membrane potential gradient. Modification of the carboxylic acid functionality of AOA to an ester and amide functionality will also allow for comparison to AOA inhibitor activity once tested in animals. Another modification of AOA was made by converting the oxyamino functionality into a hydroxylamino functionality, in an effort to enhance the bioactivity and drug-properties of the compound. In addition, the CBS product, L, L-cystathionine, was modified by converting the amino group of the serine (or cysteine) derived half of the product into a carbonyl group, although purification was not successful. Both of the aforementioned modifications will allow for comparison to natural inhibitor and product binding activities, respectively."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10657/689"],"dc:language.iso":["eng"],"dc:rights":["The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s)."],"dc:subject":["Cystathionine β-synthase","Colorectal cancer","Aminooxyacetic acid"],"dc:title":["Synthesis of Inhibitors of Cystathionine β-Synthase"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["University of Houston"]},"updated_at":"2026-07-24T02:32:34Z"}