{"id":{"repo_id":"houston","oai_identifier":"oai:uh-ir.tdl.org:10657/5690"},"canonical_url":"https://search.dev.ndltd.org/etd/houston/oai:uh-ir.tdl.org:10657/5690","repository":{"repo_id":"houston","name":"University of Houston","base_url":"https://uh-ir.tdl.org/server/oai/request"},"display":{"title":"Bedside Washing of Stored Red Blood Cells: A Simple Apparatus Based on Microscale Sedimentation in Normal Gravity","abstract":"The aim of this study was to develop and evaluate a simple, inexpensive bedside RBC washer with the capability to process multiple pRBC units within a short period without any alteration to current blood collection and storage practices. Washing pRBC units prior to transfusion is recommended for hypersensitive patients who have a higher susceptibility towards Allergic Transfusion Reactions (ATR) due to residual plasma proteins. Unfortunately, conventional washing is bulky, expensive and laborious, due to concentrating washed units through centrifugation. In response, we developed a bedside RBC washer that utilizes gravitational sedimentation of RBCs within a saline suspension. We evaluated its ability to concentrate washed pRBC units to a therapeutic hematocrit of 65%, but it optimally concentrated to a physiological hematocrit of 41%. Concentration was found to be dependent on initial morphology of the unit. In terms of improved quality, the gravity-driven washing system performed comparably with conventional washing.","abstract_html":"The aim of this study was to develop and evaluate a simple, inexpensive bedside RBC washer with the capability to process multiple pRBC units within a short period without any alteration to current blood collection and storage practices. Washing pRBC units prior to transfusion is recommended for hypersensitive patients who have a higher susceptibility towards Allergic Transfusion Reactions (ATR) due to residual plasma proteins. Unfortunately, conventional washing is bulky, expensive and laborious, due to concentrating washed units through centrifugation. In response, we developed a bedside RBC washer that utilizes gravitational sedimentation of RBCs within a saline suspension. We evaluated its ability to concentrate washed pRBC units to a therapeutic hematocrit of 65%, but it optimally concentrated to a physiological hematocrit of 41%. Concentration was found to be dependent on initial morphology of the unit. In terms of improved quality, the gravity-driven washing system performed comparably with conventional washing.","abstract_has_math":false,"creators":["Huynh, Rose Ann A."],"institution":"University of Houston","degree_name":"Master of Science","degree_level":"Masters","degree_discipline":"Biomedical Engineering","degree_department":null,"school":null,"contributors":[],"advisors":["Shevkoplyas, Sergey S."],"committee_chairs":[],"committee_members":["Mohan, Chandra","George, Alex","Wu, Tianfu"],"year":2015,"date_issued":"2015-05","date_published":"2015-05","updated_at":"2026-07-24T02:32:27Z","subjects":["Bedside Washing","Stored Red Blood Cells"],"languages":["eng"],"rights":["The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s)."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10657/5690","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Shevkoplyas, Sergey S."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Mohan, Chandra","George, Alex","Wu, Tianfu"]},{"key":"dc:creator","label":"Author","values":["Huynh, Rose Ann A."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2020-01-03T23:14:23Z"]},{"key":"dc:date.issued","label":"Date","values":["2015-05"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Houston"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bedside Washing","Stored Red Blood Cells"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s)."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10657/5690"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The aim of this study was to develop and evaluate a simple, inexpensive bedside RBC washer with the capability to process multiple pRBC units within a short period without any alteration to current blood collection and storage practices. Washing pRBC units prior to transfusion is recommended for hypersensitive patients who have a higher susceptibility towards Allergic Transfusion Reactions (ATR) due to residual plasma proteins. Unfortunately, conventional washing is bulky, expensive and laborious, due to concentrating washed units through centrifugation. In response, we developed a bedside RBC washer that utilizes gravitational sedimentation of RBCs within a saline suspension. We evaluated its ability to concentrate washed pRBC units to a therapeutic hematocrit of 65%, but it optimally concentrated to a physiological hematocrit of 41%. Concentration was found to be dependent on initial morphology of the unit. In terms of improved quality, the gravity-driven washing system performed comparably with conventional washing."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Bedside Washing of Stored Red Blood Cells: A Simple Apparatus Based on Microscale Sedimentation in Normal Gravity"]}]}],"canonical_facts":{"dc:contributor.advisor":["Shevkoplyas, Sergey S."],"dc:contributor.committeemember":["Mohan, Chandra","George, Alex","Wu, Tianfu"],"dc:creator":["Huynh, Rose Ann A."],"dc:date.accessioned":["2020-01-03T23:14:23Z"],"dc:date.issued":["2015-05"],"dc:description.abstract":["The aim of this study was to develop and evaluate a simple, inexpensive bedside RBC washer with the capability to process multiple pRBC units within a short period without any alteration to current blood collection and storage practices. Washing pRBC units prior to transfusion is recommended for hypersensitive patients who have a higher susceptibility towards Allergic Transfusion Reactions (ATR) due to residual plasma proteins. Unfortunately, conventional washing is bulky, expensive and laborious, due to concentrating washed units through centrifugation. In response, we developed a bedside RBC washer that utilizes gravitational sedimentation of RBCs within a saline suspension. We evaluated its ability to concentrate washed pRBC units to a therapeutic hematocrit of 65%, but it optimally concentrated to a physiological hematocrit of 41%. Concentration was found to be dependent on initial morphology of the unit. In terms of improved quality, the gravity-driven washing system performed comparably with conventional washing."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/10657/5690"],"dc:language.iso":["eng"],"dc:rights":["The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s)."],"dc:subject":["Bedside Washing","Stored Red Blood Cells"],"dc:title":["Bedside Washing of Stored Red Blood Cells: A Simple Apparatus Based on Microscale Sedimentation in Normal Gravity"],"thesis:degree_discipline":["Biomedical Engineering"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["University of Houston"]},"updated_at":"2026-07-24T02:32:27Z"}