High-Magnification Digital Staining of FTIR Spectroscopic Images

Histopathology, the examination of molecular and microscopic structures, is essential in tissue analysis and disease diagnosis. It currently relies on the use of dyes and stains to label the morphology and the chemical composition of tissue samples. It relies heavily on human interpretation of the tissue and the chemical staining process has several limitations in histopathologic examinations. FTIR spectroscopic imaging has seen groundbreaking success in the characterization of tissue, but has yet to provide practical pathological examination of biopsies. In an attempt to build a bridge between modern spectroscopic imaging techniques and current clinical research, digital staining aims to make a rapid and quantitative solution available by providing additional molecular and morphological information for pathology. In this work, we develop digital staining techniques that can be applied to high-magnification samples collected using Fourier-transform infrared (FTIR) and discrete-frequency infrared (DFIR) images.

Name thesis:degree_name

Master of Science in Electrical Engineering

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Electrical Engineering

Grantor

University of Houston

Year dc:date.issued

2017

Author dc:creator

• Daeinejad, Seyeddavar

Advisor dc:contributor.advisor

• Mayerich, David

Committee members dc:contributor.committeemember

• Shih, Wei-Chuan
• Eriksen, Jason
• Nguyen, Hien Van

• Conventional histopathology
• Fourier transforms
• Digital staining

Statement dc:rights

• The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/10657/2943

OAI identifier oai:identifier

oai:uh-ir.tdl.org:10657/2943