{"id":{"repo_id":"houston","oai_identifier":"oai:uh-ir.tdl.org:10657/2194"},"canonical_url":"https://search.dev.ndltd.org/etd/houston/oai:uh-ir.tdl.org:10657/2194","repository":{"repo_id":"houston","name":"University of Houston","base_url":"https://uh-ir.tdl.org/server/oai/request"},"display":{"title":"Quantification and Molecular Characterization of Anti-citrulline Antibodies in Rheumatoid Arthritis","abstract":"Autoimmune diseases are believed to result from the inability of the immune system to distinguish self and non-self. Rheumatoid Arthritis (RA) is a systemic autoimmune disease that leads to joint destruction and affects more than 1.5 million adults (Centre for Disease Control). Auto-antibodies against citrullinated proteins (ACPA) are present in approximately 60-75% of RA patients with 96% specificity. Although the importance of ACPA, both as a causative agent and diagnostic marker, has been established, the reactivities of single ACPA and the eptiopes are unknown. Isolation and characterization of protein targets of ACPA would shed light on the underlying mechanism of RA and would offer (i) earlier diagnosis and (ii) routes for therapeutic intervention. Here, we employ a novel high-throughput methodology, microengraving, based on fabricated nanowells arrays, to isolate antibodies from stimulated memory B cells of RA patients. In conjunction with single-cell RT-PCR amplification this technique is employed to perform molecular characterization of ACPA antibodies.","abstract_html":"Autoimmune diseases are believed to result from the inability of the immune system to distinguish self and non-self. Rheumatoid Arthritis (RA) is a systemic autoimmune disease that leads to joint destruction and affects more than 1.5 million adults (Centre for Disease Control). Auto-antibodies against citrullinated proteins (ACPA) are present in approximately 60-75% of RA patients with 96% specificity. Although the importance of ACPA, both as a causative agent and diagnostic marker, has been established, the reactivities of single ACPA and the eptiopes are unknown. Isolation and characterization of protein targets of ACPA would shed light on the underlying mechanism of RA and would offer (i) earlier diagnosis and (ii) routes for therapeutic intervention. Here, we employ a novel high-throughput methodology, microengraving, based on fabricated nanowells arrays, to isolate antibodies from stimulated memory B cells of RA patients. In conjunction with single-cell RT-PCR amplification this technique is employed to perform molecular characterization of ACPA antibodies.","abstract_has_math":false,"creators":["Lie, Anthony 1987-"],"institution":"University of Houston","degree_name":"Master of Science","degree_level":"Masters","degree_discipline":"Chemical Engineering","degree_department":null,"school":null,"contributors":[],"advisors":["Varadarajan, Navin"],"committee_chairs":[],"committee_members":["Krishnamoorti, Ramanan","Economou, Demetre J."],"year":2012,"date_issued":"2012-08","date_published":"2012-08","updated_at":"2026-07-24T02:31:42Z","subjects":["Rheumatoid Arthritis","ACPA","Auto-antibodies","CCP","Nanowell arrays","Single cell"],"languages":["eng"],"rights":["The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. 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UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s)."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10657/2194"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Autoimmune diseases are believed to result from the inability of the immune system to distinguish self and non-self. Rheumatoid Arthritis (RA) is a systemic autoimmune disease that leads to joint destruction and affects more than 1.5 million adults (Centre for Disease Control). Auto-antibodies against citrullinated proteins (ACPA) are present in approximately 60-75% of RA patients with 96% specificity. Although the importance of ACPA, both as a causative agent and diagnostic marker, has been established, the reactivities of single ACPA and the eptiopes are unknown. Isolation and characterization of protein targets of ACPA would shed light on the underlying mechanism of RA and would offer (i) earlier diagnosis and (ii) routes for therapeutic intervention. Here, we employ a novel high-throughput methodology, microengraving, based on fabricated nanowells arrays, to isolate antibodies from stimulated memory B cells of RA patients. In conjunction with single-cell RT-PCR amplification this technique is employed to perform molecular characterization of ACPA antibodies."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Quantification and Molecular Characterization of Anti-citrulline Antibodies in Rheumatoid Arthritis"]}]}],"canonical_facts":{"dc:contributor.advisor":["Varadarajan, Navin"],"dc:contributor.committeemember":["Krishnamoorti, Ramanan","Economou, Demetre J."],"dc:creator":["Lie, Anthony 1987-"],"dc:date.accessioned":["2018-02-15T20:10:13Z"],"dc:date.available":["2018-02-15T20:10:13Z"],"dc:date.issued":["2012-08"],"dc:description.abstract":["Autoimmune diseases are believed to result from the inability of the immune system to distinguish self and non-self. Rheumatoid Arthritis (RA) is a systemic autoimmune disease that leads to joint destruction and affects more than 1.5 million adults (Centre for Disease Control). Auto-antibodies against citrullinated proteins (ACPA) are present in approximately 60-75% of RA patients with 96% specificity. Although the importance of ACPA, both as a causative agent and diagnostic marker, has been established, the reactivities of single ACPA and the eptiopes are unknown. Isolation and characterization of protein targets of ACPA would shed light on the underlying mechanism of RA and would offer (i) earlier diagnosis and (ii) routes for therapeutic intervention. Here, we employ a novel high-throughput methodology, microengraving, based on fabricated nanowells arrays, to isolate antibodies from stimulated memory B cells of RA patients. In conjunction with single-cell RT-PCR amplification this technique is employed to perform molecular characterization of ACPA antibodies."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10657/2194"],"dc:language.iso":["eng"],"dc:rights":["The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s)."],"dc:subject":["Rheumatoid Arthritis","ACPA","Auto-antibodies","CCP","Nanowell arrays","Single cell"],"dc:title":["Quantification and Molecular Characterization of Anti-citrulline Antibodies in Rheumatoid Arthritis"],"thesis:degree_discipline":["Chemical Engineering"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["University of Houston"]},"updated_at":"2026-07-24T02:31:42Z"}