Heartbeat Optical Coherence Elastography: A Method for Passive Biomechanical Assessment of the Cornea

In this dissertation, the biomechanical properties of the cornea are explored using a novel, completely passive, biomechanical analysis technique termed heartbeat optical coherence elastography (Hb-OCE). This technology enables mechanical characterization of the cornea with applications in disease detection, staging, and therapeutic monitoring. This dissertation explores the development and evolution of the Hb-OCE technique and is divided accordingly. In the first chapter, the need for biomechanical analysis in ocular health and diagnostics and the tools available to perform that task are discussed. The second chapter describes the development of the Hb-OCE technique. Hb-OCE was initially demonstrated in a proof-of-concept ex vivo investigation. The mechanical properties of the cornea were measured in response to a simulated ocular pulse. The capabilities of the technique were initially demonstrated, including its ability to identify spatial mechanical contrast in a heterogenous sample. In the next chapter, the Hb-OCE technique is compared to better established optical elastography techniques to investigate the effects of collagen XII deficiency on the biomechanical properties of the murine cornea ex vivo. Hb-OCE was successfully used to distinguish collagen XII deficient tissue from the wild-type, and the ability of Hb-OCE to detect tissue elastic nonlinearity was demonstrated. In the fourth chapter, Hb-OCE is demonstrated in vivo, successfully illustrating that not only can the technique detect mechanical contrast in vivo, but corneal stiffness can be measured using only the heartbeat-induced ocular pulse as a source of displacement. The fifth chapter discusses how Hb-OCE was compared to a similar quasi-static but active elastography technique known as compression elastography. Furthermore, stiffness mapping using Hb-OCE was demonstrated for the first time. Finally, this dissertation concludes with closing thoughts on areas of technical improvements and future applications.

Name thesis:degree_name

Doctor of Philosophy

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Biomedical Engineering

Grantor

University of Houston

Year dc:date.issued

2022

Author dc:creator

• Nair, Achuth

Advisor dc:contributor.advisor

• Larin, Kirill V.

Committee members dc:contributor.committeemember

• Aglyamov, Salavat R.
• Twa, Michael D.
• Yoon, Geunyoung
• Zhang, Yingchun
• Ince, Nuri F.

• Cornea
• Biomechanics
• Elastography
• Optical coherence tomography
• Optical coherence elastography

Statement dc:rights

• The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. UH Libraries has secured permission to reproduce any and all previously published materials contained in the work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/10657/13339

OAI identifier oai:identifier

oai:uh-ir.tdl.org:10657/13339