Development of a head-mount fiber scanning system for imaging in vivo
In this thesis I will describe the basic concepts necessary for acquiring functional data from nerve tissue by application of two-photon laser scanning microscopy and its miniaturization for in vivo imaging in freely moving animals. The construction of a laser with enhanced repetition rate and the design of a novel non-resonant fiber scanning technique is emphasized. The increased repetition rate of the femtosecond laser source should decrease undesirable non-linear effects when transmitting ultra-short pulses with a single mode fiber. By implementing the prototype of a head-mounted microscope involving the novel fiber scanner and testing with fluorescent probes I can demonstrate its feasibility - not only for imaging in freely moving animals but for mobile laser scanning microscopy in general.
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