{"id":{"repo_id":"heid-diss","oai_identifier":"oai:archiv.ub.uni-heidelberg.de:1413"},"canonical_url":"https://search.dev.ndltd.org/etd/heid-diss/oai:archiv.ub.uni-heidelberg.de:1413","repository":{"repo_id":"heid-diss","name":"Universität Heidelberg","base_url":"http://archiv.ub.uni-heidelberg.de/volltextserver/cgi/oai2"},"display":{"title":"Biophysical analysis of the spatial and temporal distribution of free Ca2+ ions within micro- and nanodomains of muscle cells","abstract":"This work establishes methods to measure and quantify the spatial and temporal changes of the free Ca2+ ion concentration ([Ca2+]) inside skeletal muscle fibers and to calculate the underlying Ca2+ release from the sarcoplasmic reticulum (SR) caused by the opening of Ca2+ permeable ion channels (RyRs). The recording of microdomain Ca2+ signals (spatially averaged measurements of transient increases in myoplasmic [Ca2+]), was achieved by epi-illumination microscopy utilizing changes in the absorption of the Ca2+ indicator Antipyrylazo III. Nanodomain Ca2+ signals, the brief and highly localized spatio-temporal changes in [Ca2+] caused by elementary Ca2+ release events, were measured with submicron precision and a time resolution of 2 ms using confocal laser scanning fluorescence microscopy. The underlying release rate of Ca2+ ions from the SR was calculated using the most detailed information about intracellular Ca2+ buffers and Ca2+ pumps currently available. With these quantitative tools this work lead to the conclusion that the elementary events of Ca2+ release are generated by the opening of more than one RyR. Striking morphological differences between elementary Ca2+ release events measured under different experimental conditions are revealed, which can be explained by the molecular structure of the Ca2+ release source. For the first time this work presents measurements of Ca2+ sparks in adult mammalian muscle with high abundance which is of significant importance for medical diseases involving pathophysiological changes in Ca2+ regulation.","abstract_html":"This work establishes methods to measure and quantify the spatial and temporal changes of the free Ca2+ ion concentration ([Ca2+]) inside skeletal muscle fibers and to calculate the underlying Ca2+ release from the sarcoplasmic reticulum (SR) caused by the opening of Ca2+ permeable ion channels (RyRs). The recording of microdomain Ca2+ signals (spatially averaged measurements of transient increases in myoplasmic [Ca2+]), was achieved by epi-illumination microscopy utilizing changes in the absorption of the Ca2+ indicator Antipyrylazo III. Nanodomain Ca2+ signals, the brief and highly localized spatio-temporal changes in [Ca2+] caused by elementary Ca2+ release events, were measured with submicron precision and a time resolution of 2 ms using confocal laser scanning fluorescence microscopy. The underlying release rate of Ca2+ ions from the SR was calculated using the most detailed information about intracellular Ca2+ buffers and Ca2+ pumps currently available. With these quantitative tools this work lead to the conclusion that the elementary events of Ca2+ release are generated by the opening of more than one RyR. Striking morphological differences between elementary Ca2+ release events measured under different experimental conditions are revealed, which can be explained by the molecular structure of the Ca2+ release source. For the first time this work presents measurements of Ca2+ sparks in adult mammalian muscle with high abundance which is of significant importance for medical diseases involving pathophysiological changes in Ca2+ regulation.","abstract_has_math":false,"creators":["Kirsch, Wolfgang G."],"institution":"Universität Heidelberg","degree_name":null,"degree_level":"thesis.doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Fink, Rainer H. A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2000,"date_issued":"2000-10-27","date_published":"2000-10-27","updated_at":"2026-07-24T02:28:55Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://www.ub.uni-heidelberg.de/archiv/1413","outbound_label":"Repository record","outbound_source":"source_url"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Fink, Rainer H. 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The recording of microdomain Ca2+ signals (spatially averaged measurements of transient increases in myoplasmic [Ca2+]), was achieved by epi-illumination microscopy utilizing changes in the absorption of the Ca2+ indicator Antipyrylazo III. Nanodomain Ca2+ signals, the brief and highly localized spatio-temporal changes in [Ca2+] caused by elementary Ca2+ release events, were measured with submicron precision and a time resolution of 2 ms using confocal laser scanning fluorescence microscopy. The underlying release rate of Ca2+ ions from the SR was calculated using the most detailed information about intracellular Ca2+ buffers and Ca2+ pumps currently available. With these quantitative tools this work lead to the conclusion that the elementary events of Ca2+ release are generated by the opening of more than one RyR. Striking morphological differences between elementary Ca2+ release events measured under different experimental conditions are revealed, which can be explained by the molecular structure of the Ca2+ release source. For the first time this work presents measurements of Ca2+ sparks in adult mammalian muscle with high abundance which is of significant importance for medical diseases involving pathophysiological changes in Ca2+ regulation."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Biophysical analysis of the spatial and temporal distribution of free Ca2+ ions within micro- and nanodomains of muscle cells","Biophysikalische Analyse der räumlichen und zeitlichen Verteilung freier Ca2+-Ionen in Mikro- und Nanodomänen von Muskelzellen"]}]}],"canonical_facts":{"dc:contributor":["Fink, Rainer H. A."],"dc:creator":["Kirsch, Wolfgang G."],"dc:description.abstract":["This work establishes methods to measure and quantify the spatial and temporal changes of the free Ca2+ ion concentration ([Ca2+]) inside skeletal muscle fibers and to calculate the underlying Ca2+ release from the sarcoplasmic reticulum (SR) caused by the opening of Ca2+ permeable ion channels (RyRs). The recording of microdomain Ca2+ signals (spatially averaged measurements of transient increases in myoplasmic [Ca2+]), was achieved by epi-illumination microscopy utilizing changes in the absorption of the Ca2+ indicator Antipyrylazo III. Nanodomain Ca2+ signals, the brief and highly localized spatio-temporal changes in [Ca2+] caused by elementary Ca2+ release events, were measured with submicron precision and a time resolution of 2 ms using confocal laser scanning fluorescence microscopy. The underlying release rate of Ca2+ ions from the SR was calculated using the most detailed information about intracellular Ca2+ buffers and Ca2+ pumps currently available. With these quantitative tools this work lead to the conclusion that the elementary events of Ca2+ release are generated by the opening of more than one RyR. Striking morphological differences between elementary Ca2+ release events measured under different experimental conditions are revealed, which can be explained by the molecular structure of the Ca2+ release source. For the first time this work presents measurements of Ca2+ sparks in adult mammalian muscle with high abundance which is of significant importance for medical diseases involving pathophysiological changes in Ca2+ regulation."],"dc:format.medium":["application/pdf"],"dc:publisher":["Universitätsbibliothek Heidelberg"],"dc:title":["Biophysical analysis of the spatial and temporal distribution of free Ca2+ ions within micro- and nanodomains of muscle cells","Biophysikalische Analyse der räumlichen und zeitlichen Verteilung freier Ca2+-Ionen in Mikro- und Nanodomänen von Muskelzellen"],"dc:type":["doctoralThesis"],"thesis:degree_level":["thesis.doctoral"],"thesis:institution_name":["Universität Heidelberg"]},"updated_at":"2026-07-24T02:28:55Z"}