{"id":{"repo_id":"guelph","oai_identifier":"oai:atrium.lib.uoguelph.ca:10214/13002"},"canonical_url":"https://search.dev.ndltd.org/etd/guelph/oai:atrium.lib.uoguelph.ca:10214/13002","repository":{"repo_id":"guelph","name":"University of Guelph","base_url":"https://atrium.lib.uoguelph.ca/server/oai/request"},"display":{"title":"Protective and Anti-inflammatory Effects of Protegrin-1 on Citrobacter rodentium Intestinal Infection in Mice","abstract":"Intestinal disorders and colitis affect millions of humans and food-animals world-wide. Antimicrobial peptides (AMPs) and their broad-spectrum antimicrobial activity represent a valuable potential therapeutic solution. Specifically, the potent pig-originated protegrin-1 (PG-1) has previously been shown to reduce the pathological effects of chemically induced digestive tract inflammation (colitis) along with modulation of immune responses and tissue-repair. This study aimed to extend these findings by investigating the potential protective effects of PG-1 on pathogen-induced intestinal colitis. We found that oral administration of PG-1 reduced Citrobacter rodentium intestinal infection in mice evidenced by; reduced histopathologic change in the colon, prevention of body weight loss, milder clinical signs of disease, and ultimately more effective clearance of bacterial infection relative to challenged mice. Additionally, PG-1 treatment altered the expression of various inflammatory mediators during infection to resolve inflammation and re-establish intestinal homeostasis. Interestingly, PG-1 administered in its mature form was most effective relative to the pro-form (ProPG-1).","abstract_html":"Intestinal disorders and colitis affect millions of humans and food-animals world-wide. Antimicrobial peptides (AMPs) and their broad-spectrum antimicrobial activity represent a valuable potential therapeutic solution. Specifically, the potent pig-originated protegrin-1 (PG-1) has previously been shown to reduce the pathological effects of chemically induced digestive tract inflammation (colitis) along with modulation of immune responses and tissue-repair. This study aimed to extend these findings by investigating the potential protective effects of PG-1 on pathogen-induced intestinal colitis. We found that oral administration of PG-1 reduced Citrobacter rodentium intestinal infection in mice evidenced by; reduced histopathologic change in the colon, prevention of body weight loss, milder clinical signs of disease, and ultimately more effective clearance of bacterial infection relative to challenged mice. Additionally, PG-1 treatment altered the expression of various inflammatory mediators during infection to resolve inflammation and re-establish intestinal homeostasis. Interestingly, PG-1 administered in its mature form was most effective relative to the pro-form (ProPG-1).","abstract_has_math":false,"creators":["Osakowicz, Celina"],"institution":"University of Guelph","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Li, Julang"],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-05-08","date_published":"2018-05-08","updated_at":"2026-08-21T16:45:07Z","subjects":["Citrobacter rodentium","colitis","Mice","Protegrin-1","antimicrobial peptide","inflammation","colon","intestine","recombinant protein","PG-1"],"languages":["en"],"rights":["Attribution 2.5 Canada"],"rights_urls":["http://creativecommons.org/licenses/by/2.5/ca/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10214/13002","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"source_record":{"url":"https://atrium.lib.uoguelph.ca/server/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai%3Aatrium.lib.uoguelph.ca%3A10214%2F13002","prefix":"dim"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Li, Julang"]},{"key":"dc:creator","label":"Author","values":["Osakowicz, Celina"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-05-08T13:56:23Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-05-08T13:56:23Z"]},{"key":"dc:date.issued","label":"Date","values":["2018-05-08"]},{"key":"dc:publisher","label":"Institution","values":["University of Guelph"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Citrobacter rodentium","colitis","Mice","Protegrin-1","antimicrobial peptide","inflammation","colon","intestine","recombinant protein","PG-1"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Attribution 2.5 Canada"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://creativecommons.org/licenses/by/2.5/ca/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10214/13002"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Intestinal disorders and colitis affect millions of humans and food-animals world-wide. Antimicrobial peptides (AMPs) and their broad-spectrum antimicrobial activity represent a valuable potential therapeutic solution. Specifically, the potent pig-originated protegrin-1 (PG-1) has previously been shown to reduce the pathological effects of chemically induced digestive tract inflammation (colitis) along with modulation of immune responses and tissue-repair. This study aimed to extend these findings by investigating the potential protective effects of PG-1 on pathogen-induced intestinal colitis. We found that oral administration of PG-1 reduced Citrobacter rodentium intestinal infection in mice evidenced by; reduced histopathologic change in the colon, prevention of body weight loss, milder clinical signs of disease, and ultimately more effective clearance of bacterial infection relative to challenged mice. Additionally, PG-1 treatment altered the expression of various inflammatory mediators during infection to resolve inflammation and re-establish intestinal homeostasis. Interestingly, PG-1 administered in its mature form was most effective relative to the pro-form (ProPG-1)."]},{"key":"dc:title","label":"Title","values":["Protective and Anti-inflammatory Effects of Protegrin-1 on Citrobacter rodentium Intestinal Infection in Mice"]}]}],"canonical_facts":{"dc:contributor.advisor":["Li, Julang"],"dc:creator":["Osakowicz, Celina"],"dc:date.accessioned":["2018-05-08T13:56:23Z"],"dc:date.available":["2018-05-08T13:56:23Z"],"dc:date.issued":["2018-05-08"],"dc:description.abstract":["Intestinal disorders and colitis affect millions of humans and food-animals world-wide. Antimicrobial peptides (AMPs) and their broad-spectrum antimicrobial activity represent a valuable potential therapeutic solution. Specifically, the potent pig-originated protegrin-1 (PG-1) has previously been shown to reduce the pathological effects of chemically induced digestive tract inflammation (colitis) along with modulation of immune responses and tissue-repair. This study aimed to extend these findings by investigating the potential protective effects of PG-1 on pathogen-induced intestinal colitis. We found that oral administration of PG-1 reduced Citrobacter rodentium intestinal infection in mice evidenced by; reduced histopathologic change in the colon, prevention of body weight loss, milder clinical signs of disease, and ultimately more effective clearance of bacterial infection relative to challenged mice. Additionally, PG-1 treatment altered the expression of various inflammatory mediators during infection to resolve inflammation and re-establish intestinal homeostasis. Interestingly, PG-1 administered in its mature form was most effective relative to the pro-form (ProPG-1)."],"dc:identifier.uri":["http://hdl.handle.net/10214/13002"],"dc:language.iso":["en"],"dc:publisher":["University of Guelph"],"dc:rights":["Attribution 2.5 Canada"],"dc:rights.uri":["http://creativecommons.org/licenses/by/2.5/ca/"],"dc:subject":["Citrobacter rodentium","colitis","Mice","Protegrin-1","antimicrobial peptide","inflammation","colon","intestine","recombinant protein","PG-1"],"dc:title":["Protective and Anti-inflammatory Effects of Protegrin-1 on Citrobacter rodentium Intestinal Infection in Mice"],"dc:type":["Thesis"]},"updated_at":"2026-08-21T16:45:07Z"}