{"id":{"repo_id":"gsu","oai_identifier":"oai:digitalcommons.georgiasouthern.edu:etd-1697"},"canonical_url":"https://search.dev.ndltd.org/etd/gsu/oai:digitalcommons.georgiasouthern.edu:etd-1697","repository":{"repo_id":"gsu","name":"Georgia Southern University","base_url":"https://digitalcommons.georgiasouthern.edu/do/oai/"},"display":{"title":"Phylogenetic Relationships within Coleeae (Bignoniaceae Juss.)","abstract":"On Madagascar, Bignoniaceae is represented by tribes Coleeae (4 genera/58 species) and Tecomeae (4 genera/15 species). Species of Coleeae occur in assemblages of sympatric, locally endemic species. The purpose of this study was to explore the phylogenetic relationships within Coleeae. In order to do so, techniques such as PCR by using universal primers for waxy nuclear gene, agarose electrophoresis, cloning and sequencing were performed. A total of 26 taxa were successfully amplified. Sequences were analyzed using three different phylogenetic programs (Maximum parsimony, maximum likelihood and Bayesian analysis) resulting in various phylogenetic trees. Main conclusions drawn from this work are: 1. waxy is a good molecular marker to resolve relationships within Coleeae; 2. ITS and waxy resolved main clades similarly showing monophyly of Colea and Rhodocolea. Since conservation requires reliable information on species identity, a good phylogenetic assessment is of great importance in determining taxonomic status of organisms.","abstract_html":"On Madagascar, Bignoniaceae is represented by tribes Coleeae (4 genera/58 species) and Tecomeae (4 genera/15 species). Species of Coleeae occur in assemblages of sympatric, locally endemic species. The purpose of this study was to explore the phylogenetic relationships within Coleeae. In order to do so, techniques such as PCR by using universal primers for waxy nuclear gene, agarose electrophoresis, cloning and sequencing were performed. A total of 26 taxa were successfully amplified. Sequences were analyzed using three different phylogenetic programs (Maximum parsimony, maximum likelihood and Bayesian analysis) resulting in various phylogenetic trees. Main conclusions drawn from this work are: 1. waxy is a good molecular marker to resolve relationships within Coleeae; 2. ITS and waxy resolved main clades similarly showing monophyly of Colea and Rhodocolea. Since conservation requires reliable information on species identity, a good phylogenetic assessment is of great importance in determining taxonomic status of organisms.","abstract_has_math":false,"creators":["Carvajal, Andreina"],"institution":null,"degree_name":"Master of Science in Biology (M.S.)","degree_level":"Thesis (open access)","degree_discipline":"Department of Biology","degree_department":null,"school":null,"contributors":["Lorenza Beati","James Hutcheon"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007-12-01T08:00:00Z","date_published":"2007-12-01T08:00:00Z","updated_at":"2026-07-24T02:27:26Z","subjects":["ETD","Bignoniaceae","Coleeae","Phylogeny","GBSSI","Waxy"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.georgiasouthern.edu/etd/697","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Lorenza Beati","James Hutcheon"]},{"key":"dc:creator","label":"Author","values":["Carvajal, Andreina"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2013-10-17T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Department of Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis (open access)"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology (M.S.)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ETD","Bignoniaceae","Coleeae","Phylogeny","GBSSI","Waxy"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.georgiasouthern.edu/etd/697"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["On Madagascar, Bignoniaceae is represented by tribes Coleeae (4 genera/58 species) and Tecomeae (4 genera/15 species). Species of Coleeae occur in assemblages of sympatric, locally endemic species. The purpose of this study was to explore the phylogenetic relationships within Coleeae. In order to do so, techniques such as PCR by using universal primers for waxy nuclear gene, agarose electrophoresis, cloning and sequencing were performed. A total of 26 taxa were successfully amplified. Sequences were analyzed using three different phylogenetic programs (Maximum parsimony, maximum likelihood and Bayesian analysis) resulting in various phylogenetic trees. Main conclusions drawn from this work are: 1. waxy is a good molecular marker to resolve relationships within Coleeae; 2. ITS and waxy resolved main clades similarly showing monophyly of Colea and Rhodocolea. Since conservation requires reliable information on species identity, a good phylogenetic assessment is of great importance in determining taxonomic status of organisms."]},{"key":"dc:title","label":"Title","values":["Phylogenetic Relationships within Coleeae (Bignoniaceae Juss.)"]}]}],"canonical_facts":{"dc:contributor":["Lorenza Beati","James Hutcheon"],"dc:creator":["Carvajal, Andreina"],"dc:date.available":["2013-10-17T07:00:00Z"],"dc:description.abstract":["On Madagascar, Bignoniaceae is represented by tribes Coleeae (4 genera/58 species) and Tecomeae (4 genera/15 species). Species of Coleeae occur in assemblages of sympatric, locally endemic species. The purpose of this study was to explore the phylogenetic relationships within Coleeae. In order to do so, techniques such as PCR by using universal primers for waxy nuclear gene, agarose electrophoresis, cloning and sequencing were performed. A total of 26 taxa were successfully amplified. Sequences were analyzed using three different phylogenetic programs (Maximum parsimony, maximum likelihood and Bayesian analysis) resulting in various phylogenetic trees. Main conclusions drawn from this work are: 1. waxy is a good molecular marker to resolve relationships within Coleeae; 2. ITS and waxy resolved main clades similarly showing monophyly of Colea and Rhodocolea. Since conservation requires reliable information on species identity, a good phylogenetic assessment is of great importance in determining taxonomic status of organisms."],"dc:identifier":["https://digitalcommons.georgiasouthern.edu/etd/697"],"dc:subject":["ETD","Bignoniaceae","Coleeae","Phylogeny","GBSSI","Waxy"],"dc:title":["Phylogenetic Relationships within Coleeae (Bignoniaceae Juss.)"],"thesis:degree_discipline":["Department of Biology"],"thesis:degree_level":["Thesis (open access)"],"thesis:degree_name":["Master of Science in Biology (M.S.)"]},"updated_at":"2026-07-24T02:27:26Z"}