{"id":{"repo_id":"greece","oai_identifier":"oai:10442/1597"},"canonical_url":"https://search.dev.ndltd.org/etd/greece/oai:10442/1597","repository":{"repo_id":"greece","name":"Greek National Archive of PhD Theses","base_url":"https://phdtheses.ekt.gr/eadd_oai/request"},"display":{"title":"Ταχεία ανίχνευση της εντεροτοξίνης του C. perfringens τύπου Α με την ανοσοενζυμική μέθοδο ELISA, σε κόπρανα ασθενών με συμπτώματα τροφικής δηλητηρίασης","abstract":"C. perfringens type A is one of the most common agents for the food poisonings in human, worldwide. Its enterotoxin produced during the sporulation is particularly considered to be responsible for the development of the food poisoning, symptoms. The detection of its enterotoxin in feces of patients with diarrhoea is a diagnostic criterion of food poisonings caused by C. perfringens type A. Therefore the application of a sensitive and rapid method as the enzyme linked immunosorbent assay (ELISA) for tracing of the enterotoxin in faecal samples was considered to be essential. In the present study an effort was made to detect the presence of enterotoxin of C. perfringens in stools. 280 faecal samples taken from patients with symptoms of food poisoning suspected to be caused by C. perfringens, were examined. All samples were tested qualitatively and quantitatively for the presence of C. perfringens and its enterotoxin. C. perfringens was recovered from 215 samples in 31 of which the microbes were >10⁶/gr of feces. Out of 26 of these 31 samples enterotoxin was detected by the sandwich ELISA method. The samples positive for enterotoxin were also examined by the reverse passive latex agglutination test. Only 22 of them were positive by this method. It is concluded that sandwich ELISA is a very sensitive method with reproductibility. Also it is rapid enough to be suggested for diagnostic purposes and epidemiological surveys.","abstract_html":"C. perfringens type A is one of the most common agents for the food poisonings in human, worldwide. Its enterotoxin produced during the sporulation is particularly considered to be responsible for the development of the food poisoning, symptoms. The detection of its enterotoxin in feces of patients with diarrhoea is a diagnostic criterion of food poisonings caused by C. perfringens type A. Therefore the application of a sensitive and rapid method as the enzyme linked immunosorbent assay (ELISA) for tracing of the enterotoxin in faecal samples was considered to be essential. In the present study an effort was made to detect the presence of enterotoxin of C. perfringens in stools. 280 faecal samples taken from patients with symptoms of food poisoning suspected to be caused by C. perfringens, were examined. All samples were tested qualitatively and quantitatively for the presence of C. perfringens and its enterotoxin. C. perfringens was recovered from 215 samples in 31 of which the microbes were >10⁶/gr of feces. Out of 26 of these 31 samples enterotoxin was detected by the sandwich ELISA method. The samples positive for enterotoxin were also examined by the reverse passive latex agglutination test. Only 22 of them were positive by this method. It is concluded that sandwich ELISA is a very sensitive method with reproductibility. Also it is rapid enough to be suggested for diagnostic purposes and epidemiological surveys.","abstract_has_math":false,"creators":["Repanta-Skaraga, Helen","Ρεπαντά-Σκαραγκά, Ελένη"],"institution":"Aristotle University Of Thessaloniki (AUTH)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1991,"date_issued":"1991","date_published":"1991","updated_at":"2026-07-24T02:25:02Z","subjects":["Ιατρική και Επιστήμες Υγείας","Βασική Ιατρική","Κλινική Ιατρική","Medical and Health Sciences","Basic Medicine","Clinical Medicine"],"languages":["gre"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["10.12681/eadd/1597"],"render_values":[{"text":"10.12681/eadd/1597","href":"https://doi.org/10.12681/eadd/1597","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10442/hedi/1597","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Repanta-Skaraga, Helen","Ρεπαντά-Σκαραγκά, Ελένη"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1991"]},{"key":"dc:publisher","label":"Institution","values":["Aristotle University Of Thessaloniki (AUTH)","Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)"]},{"key":"dc:type","label":"Dc Type","values":["PhD Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ιατρική και Επιστήμες Υγείας","Βασική Ιατρική","Κλινική Ιατρική","Medical and Health Sciences","Basic Medicine","Clinical Medicine"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["gre"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.12681/eadd/1597","http://hdl.handle.net/10442/hedi/1597"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["C. perfringens type A is one of the most common agents for the food poisonings in human, worldwide. Its enterotoxin produced during the sporulation is particularly considered to be responsible for the development of the food poisoning, symptoms. The detection of its enterotoxin in feces of patients with diarrhoea is a diagnostic criterion of food poisonings caused by C. perfringens type A. Therefore the application of a sensitive and rapid method as the enzyme linked immunosorbent assay (ELISA) for tracing of the enterotoxin in faecal samples was considered to be essential. In the present study an effort was made to detect the presence of enterotoxin of C. perfringens in stools. 280 faecal samples taken from patients with symptoms of food poisoning suspected to be caused by C. perfringens, were examined. All samples were tested qualitatively and quantitatively for the presence of C. perfringens and its enterotoxin. C. perfringens was recovered from 215 samples in 31 of which the microbes were >10⁶/gr of feces. Out of 26 of these 31 samples enterotoxin was detected by the sandwich ELISA method. The samples positive for enterotoxin were also examined by the reverse passive latex agglutination test. Only 22 of them were positive by this method. It is concluded that sandwich ELISA is a very sensitive method with reproductibility. Also it is rapid enough to be suggested for diagnostic purposes and epidemiological surveys.","To C. perfringens τύπου Α αποτελεί μία από τι πιο συχνές αιτίες τροφικών δηλητηριάσεων στον άνθρωπο, σε ολόκληρο τον κόσμο. Υπεύθυνη των συμπτωμάτων των τροφικών δηλητηριάσεων θεωρείται η εντεροτοξίνη του κλωστηριδίου, η οποία παράγεται κατά την φάση της σπορογονίας. Η ανίχνευση της εντεροτοξίνης στα κόπρανα των ασθενών αποτελεί διαγνωστικό κριτήριο των τροφικών δηλητηριάσεων από C. perfringens τύπου Α. Θεωρήθηκε λοιπόν αναγκαίο να διερευνηθεί η δυνατότητα εφαρμογής μιας ταχείας και ευαίσθητης μεθόδου για την ανίχνευση της τοξίνης αυτής. Στην παρούσα εργασία καταβλήθηκε προσπάθεια προς την κατεύθυνση αυτή. Εξετάσθηκαν 280 δείγματα ύποπτων κοπράνων ασθενών με συμπτώματα τροφικής δηλητηρίασης ύποπτης από C. perfringens. Τα δείγματα εξετάσθηκαν με ποιοτική και ποσοτική καλλιέργεια για την ανάπτυξη κλωστηριδίων και επίσης αναζητήθηκε σ' αυτά η παρουσία της εντεροτοξίνης του κλωστηριδίου. Από τα 280 δείγματα των ύποπτων κοπράνων τα 215 ήταν θετικά στην καλλιέργεια για C. perfringens. Από αυτά τα 31 έδωσαν αριθμό C. perfringens>10⁶ ανά gr κοπράνων. Η εντεροτοξίνη ανιχνεύθηκε στα 26 από τα 31 δείγματα που είχαν δώσει αριθμό C. perfringens>10⁶/gr κοπράνων με την εφαρμογή της μεθόδου Sandwich ELISA. Τα θετικά για εντεροτοξίνη δείγματα ελέγχθηκαν και με την μέθοδο της αντίστροφης παθητικής συγκόλλησης σωματιδίων latex, (RPLA). Από τα 26 δείγματα μόνο τα 22 βρέθηκαν θετικά με τη μέθοδο αυτή. Η Sandwich ELISA που εφαρμόσθηκε είναι πολύ ευαίσθητη μέθοδος, έχει επαναληψιμότητα, είναι ταχεία στην εκτέλεση της και η εφαρμογή της θα βοηθήσει τόσο στη διάγνωση των τροφικών δηλητηριάσεων όσο και σε κάθε επιδημιολογική έρευνα σχετική με τις τροφικές δηλητηριάσεις από C. perfringens τύπου Α."]},{"key":"dc:title","label":"Title","values":["Ταχεία ανίχνευση της εντεροτοξίνης του C. perfringens τύπου Α με την ανοσοενζυμική μέθοδο ELISA, σε κόπρανα ασθενών με συμπτώματα τροφικής δηλητηρίασης","Rapid detection of C. perfringens type A enterotoxion by enzyme-linked immunosorbent assay in feces from patients with food-poisoning"]}]}],"canonical_facts":{"dc:creator":["Repanta-Skaraga, Helen","Ρεπαντά-Σκαραγκά, Ελένη"],"dc:date":["1991"],"dc:description":["C. perfringens type A is one of the most common agents for the food poisonings in human, worldwide. Its enterotoxin produced during the sporulation is particularly considered to be responsible for the development of the food poisoning, symptoms. The detection of its enterotoxin in feces of patients with diarrhoea is a diagnostic criterion of food poisonings caused by C. perfringens type A. Therefore the application of a sensitive and rapid method as the enzyme linked immunosorbent assay (ELISA) for tracing of the enterotoxin in faecal samples was considered to be essential. In the present study an effort was made to detect the presence of enterotoxin of C. perfringens in stools. 280 faecal samples taken from patients with symptoms of food poisoning suspected to be caused by C. perfringens, were examined. All samples were tested qualitatively and quantitatively for the presence of C. perfringens and its enterotoxin. C. perfringens was recovered from 215 samples in 31 of which the microbes were >10⁶/gr of feces. Out of 26 of these 31 samples enterotoxin was detected by the sandwich ELISA method. The samples positive for enterotoxin were also examined by the reverse passive latex agglutination test. Only 22 of them were positive by this method. It is concluded that sandwich ELISA is a very sensitive method with reproductibility. Also it is rapid enough to be suggested for diagnostic purposes and epidemiological surveys.","To C. perfringens τύπου Α αποτελεί μία από τι πιο συχνές αιτίες τροφικών δηλητηριάσεων στον άνθρωπο, σε ολόκληρο τον κόσμο. Υπεύθυνη των συμπτωμάτων των τροφικών δηλητηριάσεων θεωρείται η εντεροτοξίνη του κλωστηριδίου, η οποία παράγεται κατά την φάση της σπορογονίας. Η ανίχνευση της εντεροτοξίνης στα κόπρανα των ασθενών αποτελεί διαγνωστικό κριτήριο των τροφικών δηλητηριάσεων από C. perfringens τύπου Α. Θεωρήθηκε λοιπόν αναγκαίο να διερευνηθεί η δυνατότητα εφαρμογής μιας ταχείας και ευαίσθητης μεθόδου για την ανίχνευση της τοξίνης αυτής. Στην παρούσα εργασία καταβλήθηκε προσπάθεια προς την κατεύθυνση αυτή. Εξετάσθηκαν 280 δείγματα ύποπτων κοπράνων ασθενών με συμπτώματα τροφικής δηλητηρίασης ύποπτης από C. perfringens. Τα δείγματα εξετάσθηκαν με ποιοτική και ποσοτική καλλιέργεια για την ανάπτυξη κλωστηριδίων και επίσης αναζητήθηκε σ' αυτά η παρουσία της εντεροτοξίνης του κλωστηριδίου. Από τα 280 δείγματα των ύποπτων κοπράνων τα 215 ήταν θετικά στην καλλιέργεια για C. perfringens. Από αυτά τα 31 έδωσαν αριθμό C. perfringens>10⁶ ανά gr κοπράνων. Η εντεροτοξίνη ανιχνεύθηκε στα 26 από τα 31 δείγματα που είχαν δώσει αριθμό C. perfringens>10⁶/gr κοπράνων με την εφαρμογή της μεθόδου Sandwich ELISA. Τα θετικά για εντεροτοξίνη δείγματα ελέγχθηκαν και με την μέθοδο της αντίστροφης παθητικής συγκόλλησης σωματιδίων latex, (RPLA). Από τα 26 δείγματα μόνο τα 22 βρέθηκαν θετικά με τη μέθοδο αυτή. Η Sandwich ELISA που εφαρμόσθηκε είναι πολύ ευαίσθητη μέθοδος, έχει επαναληψιμότητα, είναι ταχεία στην εκτέλεση της και η εφαρμογή της θα βοηθήσει τόσο στη διάγνωση των τροφικών δηλητηριάσεων όσο και σε κάθε επιδημιολογική έρευνα σχετική με τις τροφικές δηλητηριάσεις από C. perfringens τύπου Α."],"dc:identifier":["10.12681/eadd/1597","http://hdl.handle.net/10442/hedi/1597"],"dc:language":["gre"],"dc:publisher":["Aristotle University Of Thessaloniki (AUTH)","Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)"],"dc:subject":["Ιατρική και Επιστήμες Υγείας","Βασική Ιατρική","Κλινική Ιατρική","Medical and Health Sciences","Basic Medicine","Clinical Medicine"],"dc:title":["Ταχεία ανίχνευση της εντεροτοξίνης του C. perfringens τύπου Α με την ανοσοενζυμική μέθοδο ELISA, σε κόπρανα ασθενών με συμπτώματα τροφικής δηλητηρίασης","Rapid detection of C. perfringens type A enterotoxion by enzyme-linked immunosorbent assay in feces from patients with food-poisoning"],"dc:type":["PhD Thesis"]},"updated_at":"2026-07-24T02:25:02Z"}