ΜΕΛΕΤΗ ΤΩΝ ΡΙΒΟΝΟΥΚΛΕΑΣΩΝ ΤΟΥ ΟΡΟΥ ΤΟΥ ΑΙΜΑΤΟΣ ΣΕ ΥΓΙΕΙΣ ΚΑΙ ΑΣΘΕΝΕΙΣ ΜΕ ΚΑΡΚΙΝΟ ΚΑΙ ΜΙΑΣ ΠΡΩΤΕΙΝΗΣ ΠΟΥ ΠΑΡΕΜΒΑΙΝΕΙ ΣΤΗ ΔΡΑΣΗ ΤΟΥΣ

FIVE RIBONUCLEASES WERE PURIFIED FROM THE SERUM OF NORMAL HUMAN SUBJECTS. THE PROPERTIES OF FOUR OF THEM WERE IDENTICAL TO THOSE OF THE SECRETORY TYPE RIBONUCLEASES (OPTIMUM PH 8 AND FASTER HYDROLYSIS OF POLY-C COMPARED TO POLY-K). THE FIFTH ENZYME, WITH THE LOWER MOL. SIZE, HYDROLYZED FASTER POLY-K WITH AN OPTIMUMPH 7, AND THUS CAN BE CLASSIFIED TO THE NON-SECRETORY TYPE RIBONUCLEASES. THE SERUM RIBONUCLEASES OF PATIENTS WITH CANCER WERE IN GENERAL IDENTICAL WITH THOSE OF HEALTHY SUBJECTS EXCEPT FOR HEPATOMA PATIENTS WHERE THE FIFTH ENZYME WAS ALSO OF SECRETORY TYPE. A PROTEIN NAMED PRP 150 WITH MOL. SIZE 150 KD WAS PURIFIED TO HOMOGENEITY FROM HUMAN SERUM. PRP 150 IN THE PRESENCE OF RIBONUCLEASE PROTECTS RNA AGAINST ACID PRECIPITATION WITH TCA. THE PROTECTIVE ACTIVITY WAS MEASURED WITH POLY-U AS SUBSTRATE IN THE SERA OF HEALTHY INDIVIDUALS AND CANCER PATIENTS. A 2-5 FOLD INCREASE WAS OBSERVED IN THE LATTER. THE RESULTS OBTAINED, SHOWED THAT PRP 150 COULD BE A TRUSTWORTHY MARKER FOR THE DETECTION OF CANCER ANDTHE DIFFERENTIATION OF CANCER PATIENTS AND PATIENTS WITH NON-NEOPLASMIC DISEASES.

Grantor dc:publisher

Aristotle University Of Thessaloniki (AUTH)

Year dc:date

1990

Authors dc:creator

• Kouretas, Dimitrios
• Κουρέτας, Δημήτριος

• ΒΙΟΛΟΓΙΚΟΙ ΔΕΙΚΤΕΣ ΚΑΡΚΙΝΟΥ
• Καρκίνος
• ΟΡΟΣ ΑΙΜΑΤΟΣ ΑΝΘΡΩΠΟΥ
• ΠΡΟΣΤΑΣΙΑ ΤΟΥ RNA ΑΠΟ ΟΞΙΝΗ ΚΑΤΑΒΥΘΙΣΗ
• Ριβονουκλεάση
• ACID PRECIPITATION OF RNA
• Cancer
• Human serum
• Ribonuclease
• Tumor markers
• Φυσικές Επιστήμες
• Χημεία
• Natural Sciences
• Chemical Sciences

Language dc:language

gre

Identifier

10.12681/eadd/1445

OAI identifier oai:identifier

oai:10442/1445