Συσχέτιση ιστολογικών, βιοχημικών και αιματολογικών ευρημάτων μετά από χορήγηση LPS P. aeruginosa σε επίμυες wistar: Πειραματική μελέτη

This work was undertaken in order to study the behaviour of three P. aeruginosa Lipopolysaccharides (smooth, rough and deep-rough) after intravenous injection (1.5 mg) in Wistar rats. For each lipopolysaccharide 18 experimental animals were allocated and sacrificed in groups of three at 1th, 2th, 4th and 24th hours, 3th and 7th day. The organs studied by the light microscopy were: heart, lung, liver, spleen, kidneys, adrenals and eyes. In addition the distribution of the three LPSs in the liver was studied with immunochemistry and sections of liver tissue were examined in ultrastractural microscope. Bone marrow was aspirated as well as collection of whole blood for haematological and biochemical tests. Examination of tissue sections by light microscopy revealed that the earliest lesions are located in the lung and involve focal areas of atelectasis as well as infiltration of the alveolar septa by polymorphonucleocytes resulting on the 3rd day to destruction of architectural structure by massive areas of atelectasis spleen is followed with infiltration of red pulp by polymorphonuclear and eosinophils. The liver with significant lesions, necrosis, bridging fibrosis (IR) and cytoplasmic vacuoles in hepatocytes. Adrenals with cytoplasmic vacuoles mainly in cells of the fascicular zone, and kidneys with necrosis of epithelial cells of the proximal convoluted tubules. Ultrastractural examination of the liver showed swollen mitochondria, dilated canaliculi, reduction of Hglycogen, prominent lysosomes and increased of fat. Immunoperoxidase studies of the LPS distribution in liver sections showed the smooth type LPS initially only in Kupffer cells, while the rough and the deep-rough LPS were located in Kupffer cells and hepatocytes simultaneously. Biochemical analysis of serum was related to tissue damage, while the bone marrow revealed stimulation from the first hour. The result of this study show that all three P. aeruginosa LPSs are toxic, with different time action (smooth LPS actives later than rough and deep-rough LPS, but remains longer in the cells of organs) and their different inherent distribution characteristics produces the respective lesions.

Grantor dc:publisher

University of Patras

Year dc:date

1990

Authors dc:creator

• Tzanakakis, George
• Τζανακάκης, Γεώργιος

• Ιστοπαθολογικές αλλοιώσεις μετά από χορήγηση LP
• Χορήγηση LPS P. Aeruginosa σε επίμυες
• LPS P. Aeruginosa administration in rats
• Ιατρική και Επιστήμες Υγείας
• Κλινική Ιατρική
• Medical and Health Sciences
• Clinical Medicine

Language dc:language

gre

Identifier

10.12681/eadd/1318

OAI identifier oai:identifier

oai:10442/1318