Χορήγηση φαινυτοΐνης για την προφύλαξη του εγκεφάλου από υποξία

In this experimental work was studied morphologically with the light and electron microscope, whether phenytoin provides protection in cerebral hypoxia. Hypoxia (O₂ 5%, N₂O 95%) was applied in all groups of mice and rats. The rats were divided into four groups. Ar group: Hypoxia until death. Br group: Normal saline administration 20 min before hypoxia until death. Cr group: Phenytoin administration (50mg/Kg) 20 min before hypoxia until death. Dr group: Phenytoin administration (50mg/Kg) during hypoxia. Survival time and histological study of brains under light microscope were studied. The mice were divided into nine groups. Am group: Hypoxia until death. Bm group: Normal saline administration 20 min before hypoxia until death. Cm group: Phenytoin administration 20 min before hypoxia until death. Survival time and histological study of brain under light microscope were studied. Dm group: Hypoxia of a 3 min duration. Em group: Normal saline administration 20 min before hypoxia of a 3 min duration. Fm group: Phenytoin administration 20 min before hypoxia of a 3 min duration. 72 hours after the experiment hippocampus and cerebellar were taken and put in glutaraldeyde solution 3% for study by electron microscope. Gm group: Hypoxia until onset of convulsions. Hm group: Normal saline administration 20 min before hypoxia until onset of convulsions. Im group: Phenytoin administration (50 mg/Kg) 20 min before hypoxia until onset of convulsions. 72 hours after the experiment hippocampus and cerebellar were taken and put in glutaraldeyde solution 3% for two hours for study by electron microscope. Results: Phenytoin increases survival time statistically p<0.001 both in rats and mice groups. Also prevents hypoxic necrotic lesions in rats and mice brains. The most characteristic ultrastructural alterations of hypoxia groups (control groups) were: dilatation of the rough endoplasmic reticulum cisternae, edema of the mitohondria as well as ruptures of the cristae or of the external membranes, edema and dilatation of the intercellular space between the nerve fibers and cytoplasmic rarefraction of the extensions of the glial cells. In phenytoin groups it was observed in a clearly lesser degree, dilatation of the rough endoplasmic reticulum cisternae and very seldomly light edema between the nerve fibers. Conclusively phenytoin protects effectively from the effects of hypoxia.

Grantor dc:publisher

Aristotle University Of Thessaloniki (AUTH)

Year dc:date

1990

Authors dc:creator

• Maidatsi, Panagiota
• Μαϊδάτση, Παναγιώτα

• Ιατρική και Επιστήμες Υγείας
• Βασική Ιατρική
• Κλινική Ιατρική
• Medical and Health Sciences
• Basic Medicine
• Clinical Medicine

Language dc:language

gre

Identifier

10.12681/eadd/1284

OAI identifier oai:identifier

oai:10442/1284