ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΗΣ ΓΛΥΚΟΛΙΠΟΠΡΩΤΕΙΝΗΣ (G.L.P.) ΤΟΥ SLIME ΤΡΙΩΝ ΠΡΟΤΥΠΩΝ ΣΤΕΛΕΧΩΝPSEUDOMONAS AERUGINOSA ΚΑΙ ΣΥΓΚΡΙΤΙΚΗ ΜΕΛΕΤΗ ΑΥΤΗΣ ΩΣ ΠΡΟΣ ΤΙΣ ΟΜΟΙΟΤΗΤΕΣ Η ΤΙΣ ΔΙΑΦΟΡΕΣ ΜΕ ΤΟ ΛΙΠΟΠΟΛΥΣΑΚΧΑΡΙΤΗ (L.P.S.) ΤΟΥ ΜΙΚΡΟΟΡΓΑΝΙΣΜΟΥ

LIPOPOLYSACCHARIDE (LPS) SLIME AND OUTER MEMBRANES WERE OBTAINED FROM A SMOOTH NON MUCOID PSEUDOMONAS AERUGINOSA STRAIN PAC-IR AND ITS TWO ROUGH MUTANTS PAC 557 AND PAC 605. ELECTROPHORETIC ANALYSIS ON SDS- POLYACRYLAMIDE GELS AND STRAINING WITH SILVER AND COOMASSIE BLUE SHOWED DIFFERENT PROFILES BETWEEN LPS SLIME AND OUTER MEMBRANES IN ALL THREE STRAINS. COMPARATIVE ANALYSIS OF THE SACCHARIDE MOIETY, BETWEEN LPS AND SLIME OF EACH STRAIN BY HPLC DEMONSTRATED THAT THE SACCHARIDE MOIETY OF SLIME HAS DIFFERENT COMPOSITION FROM THAT OF LPS. SIX MENTALSUGARS RHAMMOSE, FUCOSE, XYLOSE, MANNOSE, GALACTOSE AND GLUCOSE WERE IDENTIFIED IN ALL THREE SLIMES, THOUGH IN DIFFERENT AMOUNTS MANNOSE AND GALACTOSE WERE NOT FOUND IN THE SMOOTH LPS WHERE AS ONLY RHAMMOSE AND GLUCOSE WERE IDENTIFIED IN THE LPS OF THE ROUGH STRAINS-COMPARATIVE ANALYSIS OF THE LIPID MOIETY BETWEENLPS AND SLIME IN ALL THREE STRAIN WITH GAS CHROMATOGRAPHY AND MASS SPECTROSCOPY INDICATED THAT LIPID MOIETY HAD NO QUALITATIVE DIFFERENCES CONCERNING THE LIPID ACIDS. FIVE LIPID ACIDS WERE IDENTIFIED IN ALL THREE SLIMES AND LPSS (-C12),2OH(-C12), (- C14), (-C16), (-C18) AND ITS ISOMERIC FORMS. AFTER GEL FILTRATION OF ALL THREE SLIMES THE POLYSACCHARIDE OBTAINS FROM THE CARBOHYDRATE PEAK FRACTION WAS FOUND TO BE PROTEIN FREE. BY GEL FILTRATION THE MOLECULAR SIZE OF THEPOLYSACCHARIDES WAS ESTIMATED TO BE ABOUT 40.000-100.000 DALTONS. THESE RESULTS SUGGESTS THAT SLIME PRODUCTION IS A COMMON PROPERTY SHARED BY BOTH SMOOTH ANDROUGH P.AERUGINOSA STRAINS. THE SACCHARIDE MOIETY OF SLIME DOES NOT REPRESENT THE SIDE CHAINS OF LPS AND IT IS PROTEIN FREE.

Grantor dc:publisher

University of Patras

Year dc:date

1989

Authors dc:creator

• Christofidou, Myrto
• Χριστοφίδου, Μυρτώ

• GLYCOLIPID (G.L.) OF P. AERUGINOSA
• GLYCOLIPOPROTEIN (G.L.P.) OF P. AERUGINOSA
• LIPOPOLYSACCHARIDE (L.P.S.) OF P. AERUGINOSA
• ΓΛΥΚΟΛΙΠΙΔΙΟ (G.L.) ΤΗΣ P. AERUGINOSA
• ΓΛΥΚΟΛΙΠΟΠΡΩΤΕΙΝΗ (G.L.P.) ΤΗΣ P. AERUGINOSA
• ΕΞΩΚΥΤΤΑΡΙΑ ΠΡΟΙΟΝΤΑ P. AERUGINOSA
• ΛΙΠΟΠΟΛΥΣΑΚΧΑΡΙΤΗΣ (L.P.S.) ΤΗΣ P. AERUGINOSA
• Ιατρική και Επιστήμες Υγείας
• Κλινική Ιατρική
• Medical and Health Sciences
• Clinical Medicine

Language dc:language

gre

Identifier

10.12681/eadd/1083

OAI identifier oai:identifier

oai:10442/1083