ΔΙΕΡΕΥΝΗΣΗ ΜΕ ΤΗΝ ΥΓΡΟΧΡΩΜΑΤΟΓΡΑΦΙΑ ΥΨΗΛΗΣ ΠΙΕΣΗΣ ΤΗΣ ΣΥΓΚΕΝΤΡΩΣΗΣ ΤΗΣ ΦΟΥΡΑΖΟΛΙΔΟΝΗΣ ΣΤΑ ΑΥΓΑ ΟΡΝΙΘΩΝ ΣΕ ΣΧΕΣΗ ΜΕ ΤΗΝ ΠΟΣΟΤΗΤΑ ΤΗΣ ΟΤΑΝ ...
IN THIS STUDY A HPLC METHOD FOR DETERMINING FURAZOLIDONE IN HENS' EGGS HAS BEENDEVELOPED. FURTHERMORE THE DEPOSITION AND CLEARANCE OF THIS DRUG IN EGGS FOLLOWING FEEDING TRIALS WITH 100, 200 AND 400 PPM FOR 28, 14 AND 14 DAYS, RESPECTIVELY, HAVE BEEN DEVELOPED. ACCORDING TO THE METHOD, THE EGG SAMPLE IS ACIDIFIED AT PH 4 AND EXTRACTED WITH DICHLOROMETHANE. THE EXTRACT IS CLARIFIED BY TREATMENT WITH ACETONE AT -78 C AND THEN BY PARTITIONING BETWEEN HEXANE AND WATER. FURAZOLIDONE IS EXTRACTED FROM THE EQUEOUS PHASE WITH DICHLOROMETHANE AND THEN IS CHROMATOGRAPHED ON A C8 COLUMN WITH A MIXTURE OF AN ACETIC ACID BUFFER, ACETONITRILE AND METHANOL (45.5+35+P9.5, V/V) AT 365 NM. THE RESULTS SHOWED THAT THE CONCENTRATION OF THE DRUG IN HEN'S EGGS DURING ITS ADMINISTRATION AT 100, 200 AND 400 PPM REACHED A PLATEAU OF APPROXIMATELY 65 PPB, 152 PPD AND 322 PPB RESPECTIVELY . AFTER CESSATION OF DRUG FEEDING THE CONCENTRATION (Y) OF FURAZOLIDONE IN EGGS AS A FUNCTION OF TIME (X DAYS) IS EXPRESSED BY THE FOLLOWING EQUATIONS:Y= 86.42 EXP (-052X) FOR HENS FED 100 PPM FENAZOLIDONE Y=125,47 EXP (0-50X) FOR HENS FED 200 PPM FURAZOLIDONE Y=177,08 EXP (0-51X) FOR HENS FED 400 PPM FURAZOLIDONE.
DRUG RESIDUESIN EGGS, FURAZOLIDONE, HENS EGGS, High pressure liquid chromatography (HPLC), ΑΥΓΑ ΟΡΝΙΘΩΝ, ΚΑΤΑΛΟΙΠΑ ΦΑΡΜΑΚΩΝ ΣΕ ΑΥΓΑ, ΥΓΡΟΧΡΩΜΑΤΟΓΡΑΦΙΑ ΥΨΗΛΗΣ ΠΙΕΣΗΣ, ΦΟΥΡΑΖΟΛΙΔΟΝΗ, Γεωπονικές Επιστήμες και Κτηνιατρική, Agricultural and Veterinary Sciences, Κτηνιατρική, Veterinary Science
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Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)