ΚΙΝΑΣΗ ΤΗΣ ΦΩΣΦΟΡΥΛΑΣΗΣ ΑΠΟ ΛΕΙΟ ΜΥΙΚΟ ΙΣΤΟ ΣΤΟΜΑΧΙΟΥ ΚΟΤΑΣ: ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΜΕΛΕΤΗ ΤΩΝ ΚΑΤΑΛΥΤΙΚΩΝ ΚΑΙ ΔΟΜΙΚΩΝ ΙΔΙΟΤΗΤΩΝ ΤΗΣ

THE SCOPE OF THIS WORK IS TO FACILITATE THE UNDERSTANDING OF ENZYMATIC REGULATION OF GLYCOGEN BREAKDOWN IN SMOOTH MUSCLE CELLS . PHOSPORYLASE KINASE FROM CHICKEN GIZZARD WAS PURIFIED BY A PROCEDURE INVOLVING AMMONIUM SULFATE FRACTIONATION 5'-AMP SEPHAROSE 4B AFFINITY CHROMATOGRAPHY COLUMN AND ULTRACENTRIFUGATION INGLYCEROL GRADIENT DENSITY CHICKEN GIZZARD PHOSPHORYLASE KINASE PRESENTS DIFFERENT STRUCTURAL AND CATALYTIC PROPERTIES IN DELATION TO THOSE FOUND FOR THE RABBIT SKELETAL MUSCLE ISOENZYME. ALTHOUGH BOTH KINASES HAVE THE SAME HIGH MOLECULAR WEIGHT (1.3 X 106), THE SUBUNIT STRUCTURE OF THESE ISOENZYMES ARE DIFFERENT. ELECTROPHORETIC DATA INDICATES THAT CHICHEN GIZZARD PHOSPHORYLASE KINASE DOES NOT COMPRISE Γ SUBUNIT IN ITS STRUCTURE WHICH IS THE CATALYTIC SUBUNIT OF RABBITSKELETAL MUSCLE ISOENZYME. GIZZARD PHOSPHORYLASE KINASE SHOWED AN EXTREMELY HIGH AFFINITY FOR ITS PROTEIN SUBSTRATE PHOSPHORYLASE B . THIS ENZYME DOES NOT EXIST IN A PHOSPHORYLATED OR ACTIVATED FORM, WHILE IS AFFECTED BY CA2+. THESE DATA SUGGEST THAT THE ACTIVATION OF CHICKEN GIZZARD PHOSPHRYLASE KINASE IS REGULATED BY A MECHANISM INVOLVING THE CA2+ SYSTEM RATHER, THAN THE PHOSPHORYLATION SYSTEM.

Grantor dc:publisher

National and Kapodistrian University of Athens

Year dc:date

1988

Authors dc:creator

• Nikolaropoulos, Stathis
• Νικολαρόπουλος, Στάθης

• Κινάση της φωσφορυλάσης
• ΛΕΙΟΙ ΜΥΕΣ
• Μεταβολισμός γλυκογόνου
• Glycogen metabolism
• Phosphorylase kinase
• SMOOTH MUSCLE
• Φυσικές Επιστήμες
• Βιολογία
• Natural Sciences
• Biological Sciences

Language dc:language

gre

Identifier

10.12681/eadd/0729

OAI identifier oai:identifier

oai:10442/0729