{"id":{"repo_id":"greece","oai_identifier":"oai:10442/0259"},"canonical_url":"https://search.dev.ndltd.org/etd/greece/oai:10442/0259","repository":{"repo_id":"greece","name":"Greek National Archive of PhD Theses","base_url":"https://phdtheses.ekt.gr/eadd_oai/request"},"display":{"title":"ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΗΣ ΚΙΝΑΣΗΣ ΚΑΖΕΙΝΗΣ ΙΙ ΑΠΟ ΣΠΛΗΝΑ ΠΑΙΔΙΩΝ ΜΕ ΜΕΣΟΓΕΙΑΚΗ ΑΝΑΙΜΙΑ","abstract":"IN THE PRESENT STUDY CASEIN KINASE II WAS PURIFIED FROM CHILDREN'S SPLEEN, SUFFERING FROM BETA-THALASSEMIA MAJOR AND WAS CHARACTERIZED SDS POLYACRYLAMIDE GEL ELECTROPHORESIS AND ISOELECTRIC FOCUSING HAVE PROVED THAT THE ENZYME WAS HIGHLY PURIFIED. THE PURIFICATION STEPS INCLUDED ION EXCHANGE CHROMATOGRAPHY, AMMONIUM SULFATE FRACTIONATION AND MOLECULAR SIEVING. THE PURIFIED ENZYME PHOSPHORYLATES IN PREFERENCE ACIDIC PROTEINS, CASEIN, USING ATP AND GTP AS PHOSPHATEDONORS, IN THE PRESENCE OF MGCL2. THE ENZYME IS CAMP AND CGMP INDEPENDENT, HAS A MOLECULAR WEIGHT OF 43.000 AND PI 71. IT IS INHIBITED BY HEPARIN. BECAUSE OF THE ABOVE CHARACTERISTICS THIS ENZYME IS LISTED AMONG CASEIN KINASES II. THE PURIFIED ENZYME CAN BE USED AS A TOOL IN THE STUDY OF PHOSPHORYLATION, ONE OF THE MOST IMPORTANT POST- SYNTHETIC MODIFICATIONS OF THE CELL MACROMOLECULES.","abstract_html":"IN THE PRESENT STUDY CASEIN KINASE II WAS PURIFIED FROM CHILDREN'S SPLEEN, SUFFERING FROM BETA-THALASSEMIA MAJOR AND WAS CHARACTERIZED SDS POLYACRYLAMIDE GEL ELECTROPHORESIS AND ISOELECTRIC FOCUSING HAVE PROVED THAT THE ENZYME WAS HIGHLY PURIFIED. THE PURIFICATION STEPS INCLUDED ION EXCHANGE CHROMATOGRAPHY, AMMONIUM SULFATE FRACTIONATION AND MOLECULAR SIEVING. THE PURIFIED ENZYME PHOSPHORYLATES IN PREFERENCE ACIDIC PROTEINS, CASEIN, USING ATP AND GTP AS PHOSPHATEDONORS, IN THE PRESENCE OF MGCL2. THE ENZYME IS CAMP AND CGMP INDEPENDENT, HAS A MOLECULAR WEIGHT OF 43.000 AND PI 71. IT IS INHIBITED BY HEPARIN. BECAUSE OF THE ABOVE CHARACTERISTICS THIS ENZYME IS LISTED AMONG CASEIN KINASES II. THE PURIFIED ENZYME CAN BE USED AS A TOOL IN THE STUDY OF PHOSPHORYLATION, ONE OF THE MOST IMPORTANT POST- SYNTHETIC MODIFICATIONS OF THE CELL MACROMOLECULES.","abstract_has_math":false,"creators":["Gounari, Antonia","Γούναρη, Αντωνία"],"institution":"National and Kapodistrian University of Athens","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1986,"date_issued":"1986","date_published":"1986","updated_at":"2026-07-24T02:25:08Z","subjects":["Βιοχημεία","ΚΙΝΑΣΗ ΚΑΖΕΙΝΗΣ","Μεσογειακή αναιμία","Σπλήνας","Φωσφορυλίωση","BETA THALASSEMIA MAJOR","Casein kinase","Phosphorylation","Spleen","Φυσικές Επιστήμες","Βιολογία","Natural Sciences","Biological Sciences"],"languages":["gre"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["10.12681/eadd/0259"],"render_values":[{"text":"10.12681/eadd/0259","href":"https://doi.org/10.12681/eadd/0259","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10442/hedi/0259","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Gounari, Antonia","Γούναρη, Αντωνία"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1986"]},{"key":"dc:publisher","label":"Institution","values":["National and Kapodistrian University of Athens","Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)"]},{"key":"dc:type","label":"Dc Type","values":["PhD Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Βιοχημεία","ΚΙΝΑΣΗ ΚΑΖΕΙΝΗΣ","Μεσογειακή αναιμία","Σπλήνας","Φωσφορυλίωση","BETA THALASSEMIA MAJOR","Casein kinase","Phosphorylation","Spleen","Φυσικές Επιστήμες","Βιολογία","Natural Sciences","Biological Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["gre"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.12681/eadd/0259","http://hdl.handle.net/10442/hedi/0259"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["IN THE PRESENT STUDY CASEIN KINASE II WAS PURIFIED FROM CHILDREN'S SPLEEN, SUFFERING FROM BETA-THALASSEMIA MAJOR AND WAS CHARACTERIZED SDS POLYACRYLAMIDE GEL ELECTROPHORESIS AND ISOELECTRIC FOCUSING HAVE PROVED THAT THE ENZYME WAS HIGHLY PURIFIED. THE PURIFICATION STEPS INCLUDED ION EXCHANGE CHROMATOGRAPHY, AMMONIUM SULFATE FRACTIONATION AND MOLECULAR SIEVING. THE PURIFIED ENZYME PHOSPHORYLATES IN PREFERENCE ACIDIC PROTEINS, CASEIN, USING ATP AND GTP AS PHOSPHATEDONORS, IN THE PRESENCE OF MGCL2. THE ENZYME IS CAMP AND CGMP INDEPENDENT, HAS A MOLECULAR WEIGHT OF 43.000 AND PI 71. IT IS INHIBITED BY HEPARIN. BECAUSE OF THE ABOVE CHARACTERISTICS THIS ENZYME IS LISTED AMONG CASEIN KINASES II. THE PURIFIED ENZYME CAN BE USED AS A TOOL IN THE STUDY OF PHOSPHORYLATION, ONE OF THE MOST IMPORTANT POST- SYNTHETIC MODIFICATIONS OF THE CELL MACROMOLECULES.","Σ'ΑΥΤΗ ΤΗΝ ΕΡΓΑΣΙΑ ΑΠΟΜΟΝΩΘΗΚΕ ΚΑΙ ΜΕΛΕΤΗΘΗΚΕ Η ΚΙΝΑΣΗ ΚΑΖΕΙΝΗΣ ΙΙ, ΑΠΟ ΣΠΛΗΝΑΠΑΙΔΙΩΝ ΜΕ ΜΕΣΟΓΕΙΑΚΗ ΑΝΑΙΜΙΑ. Η ΗΛΕΚΤΡΟΦΟΡΗΣΗ ΣΕ ΣΥΝΘΗΚΕΣ ΑΠΟΔΙΑΤΑΞΗΣ ΚΑΙ Η ΙΣΟΗΛΕΚΤΡΙΚΗ ΕΣΤΙΑΣΗ, ΕΔΕΙΞΑΝ ΟΤΙ Ο ΚΑΘΑΡΙΣΜΟΣ ΗΤΑΝ ΥΨΗΛΗΣ ΑΠΟΔΟΣΗΣ. ΤΑ ΣΤΑΔΙΑΚΑΘΑΡΙΣΜΟΥ ΠΕΡΙΕΛΑΜΒΑΝΑΝ ΣΤΗΛΕΣ ΙΟΝΤΟΑΝΤΑΛΛΑΓΗΣ, ΜΟΡΙΑΚΗΣ ΔΙΗΘΗΣΗΣ ΚΑΙ ΚΛΑΣΜΑΤΩΣΗ ΜΕ ΘΕΙΙΚΟ ΑΜΜΩΝΙΟ. ΤΟ ΕΝΖΥΜΟ ΠΟΥ ΑΠΟΜΟΝΩΘΗΚΕ ΦΩΣΦΟΡΥΛΙΩΝΕΙ ΚΑΤΑ ΠΡΟΤΙΜΗΣΗΟΞΙΝΕΣ ΠΡΩΤΕΙΝΕΣ, ΚΑΖΕΙΝΗ, ΧΡΗΣΙΜΟΠΟΙΩΝΤΑΣ ΔΟΤΗ ΦΩΣΦΟΡΟΥ ΑΤΡ 'Η GTP, ΠΑΡΟΥΣΙΑMGCL2. ΔΕΝ ΕΞΑΡΤΑΤΑΙ ΑΠΟ CAMP # CGMP, ΕΧΕΙ ΜΟΡΙΑΚΟ ΒΑΡΟΣ 43.000 ΡΙ 71 ΚΑΙ ΑΝΑΣΤΕΛΛΕΤΑΙ Η ΔΡΑΣΗ ΤΟΥ ΠΑΡΟΥΣΙΑ ΗΠΑΡΙΝΗΣ. ΟΙ ΠΑΡΑΠΑΝΩ ΙΔΙΟΤΗΤΕΣ, ΤΟ ΚΑΤΑΤΑΣΣΟΥΝ ΣΤΙΣ ΚΙΝΑΣΕΣ ΚΑΖΕΙΝΗΣ ΙΙ ΚΑΙ ΤΟ ΚΑΘΙΣΤΟΥΝ ΙΚΑΝΟ ΝΑ ΧΡΗΣΙΜΕΥΣΕΙ ΣΑΝ ΑΠΑΡΑΙΤΗΤΟ ΕΡΓΑΛΕΙΟ ΓΙΑ ΤΗ ΜΕΛΕΤΗ ΤΟΥ ΜΗΧΑΝΙΣΜΟΥ ΤΗΣ ΦΩΣΦΟΡΥΛΙΩΣΗΣ, ΠΟΥ ΕΙΝΑΙ ΕΚΕΙΝΟΣ ΠΟΥ ΕΛΕΓΧΕΙ ΔΟΜΙΚΕΣ ΚΑΙ ΛΕΙΤΟΥΡΓΙΚΕΣ ΠΡΩΤΕΙΝΕΣ ΤΟΥ ΚΥΤΤΑΡΟΥ ΣΤΟ ΜΕΤΑΣΥΝΘΕΤΙΚΟ ΕΠΙΠΕΔΟ ΚΑΙ ΡΥΘΜΙΖΕΙ ΕΤΣΙ ΤΗΝ ΕΚΦΡΑΣΗ ΤΟΥ."]},{"key":"dc:title","label":"Title","values":["ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΗΣ ΚΙΝΑΣΗΣ ΚΑΖΕΙΝΗΣ ΙΙ ΑΠΟ ΣΠΛΗΝΑ ΠΑΙΔΙΩΝ ΜΕ ΜΕΣΟΓΕΙΑΚΗ ΑΝΑΙΜΙΑ","ISOLATION AND CHARCTERIZATION FROM SPLEAN OF CHILDREN SUFFERING FROM THALASSEMIA BETA MAJOR"]}]}],"canonical_facts":{"dc:creator":["Gounari, Antonia","Γούναρη, Αντωνία"],"dc:date":["1986"],"dc:description":["IN THE PRESENT STUDY CASEIN KINASE II WAS PURIFIED FROM CHILDREN'S SPLEEN, SUFFERING FROM BETA-THALASSEMIA MAJOR AND WAS CHARACTERIZED SDS POLYACRYLAMIDE GEL ELECTROPHORESIS AND ISOELECTRIC FOCUSING HAVE PROVED THAT THE ENZYME WAS HIGHLY PURIFIED. THE PURIFICATION STEPS INCLUDED ION EXCHANGE CHROMATOGRAPHY, AMMONIUM SULFATE FRACTIONATION AND MOLECULAR SIEVING. THE PURIFIED ENZYME PHOSPHORYLATES IN PREFERENCE ACIDIC PROTEINS, CASEIN, USING ATP AND GTP AS PHOSPHATEDONORS, IN THE PRESENCE OF MGCL2. THE ENZYME IS CAMP AND CGMP INDEPENDENT, HAS A MOLECULAR WEIGHT OF 43.000 AND PI 71. IT IS INHIBITED BY HEPARIN. BECAUSE OF THE ABOVE CHARACTERISTICS THIS ENZYME IS LISTED AMONG CASEIN KINASES II. THE PURIFIED ENZYME CAN BE USED AS A TOOL IN THE STUDY OF PHOSPHORYLATION, ONE OF THE MOST IMPORTANT POST- SYNTHETIC MODIFICATIONS OF THE CELL MACROMOLECULES.","Σ'ΑΥΤΗ ΤΗΝ ΕΡΓΑΣΙΑ ΑΠΟΜΟΝΩΘΗΚΕ ΚΑΙ ΜΕΛΕΤΗΘΗΚΕ Η ΚΙΝΑΣΗ ΚΑΖΕΙΝΗΣ ΙΙ, ΑΠΟ ΣΠΛΗΝΑΠΑΙΔΙΩΝ ΜΕ ΜΕΣΟΓΕΙΑΚΗ ΑΝΑΙΜΙΑ. Η ΗΛΕΚΤΡΟΦΟΡΗΣΗ ΣΕ ΣΥΝΘΗΚΕΣ ΑΠΟΔΙΑΤΑΞΗΣ ΚΑΙ Η ΙΣΟΗΛΕΚΤΡΙΚΗ ΕΣΤΙΑΣΗ, ΕΔΕΙΞΑΝ ΟΤΙ Ο ΚΑΘΑΡΙΣΜΟΣ ΗΤΑΝ ΥΨΗΛΗΣ ΑΠΟΔΟΣΗΣ. ΤΑ ΣΤΑΔΙΑΚΑΘΑΡΙΣΜΟΥ ΠΕΡΙΕΛΑΜΒΑΝΑΝ ΣΤΗΛΕΣ ΙΟΝΤΟΑΝΤΑΛΛΑΓΗΣ, ΜΟΡΙΑΚΗΣ ΔΙΗΘΗΣΗΣ ΚΑΙ ΚΛΑΣΜΑΤΩΣΗ ΜΕ ΘΕΙΙΚΟ ΑΜΜΩΝΙΟ. ΤΟ ΕΝΖΥΜΟ ΠΟΥ ΑΠΟΜΟΝΩΘΗΚΕ ΦΩΣΦΟΡΥΛΙΩΝΕΙ ΚΑΤΑ ΠΡΟΤΙΜΗΣΗΟΞΙΝΕΣ ΠΡΩΤΕΙΝΕΣ, ΚΑΖΕΙΝΗ, ΧΡΗΣΙΜΟΠΟΙΩΝΤΑΣ ΔΟΤΗ ΦΩΣΦΟΡΟΥ ΑΤΡ 'Η GTP, ΠΑΡΟΥΣΙΑMGCL2. ΔΕΝ ΕΞΑΡΤΑΤΑΙ ΑΠΟ CAMP # CGMP, ΕΧΕΙ ΜΟΡΙΑΚΟ ΒΑΡΟΣ 43.000 ΡΙ 71 ΚΑΙ ΑΝΑΣΤΕΛΛΕΤΑΙ Η ΔΡΑΣΗ ΤΟΥ ΠΑΡΟΥΣΙΑ ΗΠΑΡΙΝΗΣ. ΟΙ ΠΑΡΑΠΑΝΩ ΙΔΙΟΤΗΤΕΣ, ΤΟ ΚΑΤΑΤΑΣΣΟΥΝ ΣΤΙΣ ΚΙΝΑΣΕΣ ΚΑΖΕΙΝΗΣ ΙΙ ΚΑΙ ΤΟ ΚΑΘΙΣΤΟΥΝ ΙΚΑΝΟ ΝΑ ΧΡΗΣΙΜΕΥΣΕΙ ΣΑΝ ΑΠΑΡΑΙΤΗΤΟ ΕΡΓΑΛΕΙΟ ΓΙΑ ΤΗ ΜΕΛΕΤΗ ΤΟΥ ΜΗΧΑΝΙΣΜΟΥ ΤΗΣ ΦΩΣΦΟΡΥΛΙΩΣΗΣ, ΠΟΥ ΕΙΝΑΙ ΕΚΕΙΝΟΣ ΠΟΥ ΕΛΕΓΧΕΙ ΔΟΜΙΚΕΣ ΚΑΙ ΛΕΙΤΟΥΡΓΙΚΕΣ ΠΡΩΤΕΙΝΕΣ ΤΟΥ ΚΥΤΤΑΡΟΥ ΣΤΟ ΜΕΤΑΣΥΝΘΕΤΙΚΟ ΕΠΙΠΕΔΟ ΚΑΙ ΡΥΘΜΙΖΕΙ ΕΤΣΙ ΤΗΝ ΕΚΦΡΑΣΗ ΤΟΥ."],"dc:identifier":["10.12681/eadd/0259","http://hdl.handle.net/10442/hedi/0259"],"dc:language":["gre"],"dc:publisher":["National and Kapodistrian University of Athens","Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)"],"dc:subject":["Βιοχημεία","ΚΙΝΑΣΗ ΚΑΖΕΙΝΗΣ","Μεσογειακή αναιμία","Σπλήνας","Φωσφορυλίωση","BETA THALASSEMIA MAJOR","Casein kinase","Phosphorylation","Spleen","Φυσικές Επιστήμες","Βιολογία","Natural Sciences","Biological Sciences"],"dc:title":["ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΤΗΣ ΚΙΝΑΣΗΣ ΚΑΖΕΙΝΗΣ ΙΙ ΑΠΟ ΣΠΛΗΝΑ ΠΑΙΔΙΩΝ ΜΕ ΜΕΣΟΓΕΙΑΚΗ ΑΝΑΙΜΙΑ","ISOLATION AND CHARCTERIZATION FROM SPLEAN OF CHILDREN SUFFERING FROM THALASSEMIA BETA MAJOR"],"dc:type":["PhD Thesis"]},"updated_at":"2026-07-24T02:25:08Z"}