{"id":{"repo_id":"glasgow","oai_identifier":"oai:theses.gla.ac.uk:207"},"canonical_url":"https://search.dev.ndltd.org/etd/glasgow/oai:theses.gla.ac.uk:207","repository":{"repo_id":"glasgow","name":"University of Glasgow","base_url":"https://theses.gla.ac.uk/cgi/oai2"},"display":{"title":"Studies of in situ nitrosative stress following nitrate ingestion in the human upper gastrointestinal tract","abstract":"Nitrate ingestion leads to high luminal concentrations of nitric oxide being generated where saliva meets gastric acid. Nitric oxide generates N-nitrosative stress on reacting with oxygen at neutral pH. We aimed to ascertain if luminal nitric oxide exerts nitrosative stress in the upper GI tract, and to assess the influence of acid reflux. We utilised a specialised silicone tube as an epithelial model, inserting it into the upper gastro-intestinal tract of humans. Healthy volunteers were studied with and without ingestion of 15N enriched nitrate and Barrett’s oesophagus patients with and without stimulation of reflux. In volunteers, nitrate ingestion resulted in significantly higher concentrations of N-nitrosomorpholine in the tube sections exposed to acid. In Barrett’s patients, generation of N-nitrosomorpholine shifted proximally, with most nitrosative stress occurring within the oesophagus during reflux episodes. This chemistry may be harmful to patients with erosive esophagitis whose epithelium will be more sensitive to chemical mutagenesis.","abstract_html":"Nitrate ingestion leads to high luminal concentrations of nitric oxide being generated where saliva meets gastric acid. Nitric oxide generates N-nitrosative stress on reacting with oxygen at neutral pH. We aimed to ascertain if luminal nitric oxide exerts nitrosative stress in the upper GI tract, and to assess the influence of acid reflux. We utilised a specialised silicone tube as an epithelial model, inserting it into the upper gastro-intestinal tract of humans. Healthy volunteers were studied with and without ingestion of 15N enriched nitrate and Barrett’s oesophagus patients with and without stimulation of reflux. In volunteers, nitrate ingestion resulted in significantly higher concentrations of N-nitrosomorpholine in the tube sections exposed to acid. In Barrett’s patients, generation of N-nitrosomorpholine shifted proximally, with most nitrosative stress occurring within the oesophagus during reflux episodes. This chemistry may be harmful to patients with erosive esophagitis whose epithelium will be more sensitive to chemical mutagenesis.","abstract_has_math":false,"creators":["Winter, Jack Westwood"],"institution":"University of Glasgow","degree_name":null,"degree_level":"MD","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008","date_published":"2008","updated_at":"2026-07-24T02:23:14Z","subjects":["R Medicine (General)"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Winter, Jack Westwood"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2008"]},{"key":"dc:date.issued","label":"Date","values":["2008"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Glasgow"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://theses.gla.ac.uk/207/"]},{"key":"dc:relation.isreferencedby.uri","label":"Dc Relation Isreferencedby URI","values":["https://gla.on.worldcat.org/oclc/244461558"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["MD"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["R Medicine (General)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://theses.gla.ac.uk/207/1/2008WinterMD.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Nitrate ingestion leads to high luminal concentrations of nitric oxide being generated where saliva meets gastric acid. Nitric oxide generates N-nitrosative stress on reacting with oxygen at neutral pH. We aimed to ascertain if luminal nitric oxide exerts nitrosative stress in the upper GI tract, and to assess the influence of acid reflux. We utilised a specialised silicone tube as an epithelial model, inserting it into the upper gastro-intestinal tract of humans. Healthy volunteers were studied with and without ingestion of 15N enriched nitrate and Barrett’s oesophagus patients with and without stimulation of reflux. In volunteers, nitrate ingestion resulted in significantly higher concentrations of N-nitrosomorpholine in the tube sections exposed to acid. In Barrett’s patients, generation of N-nitrosomorpholine shifted proximally, with most nitrosative stress occurring within the oesophagus during reflux episodes. This chemistry may be harmful to patients with erosive esophagitis whose epithelium will be more sensitive to chemical mutagenesis."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Studies of in situ nitrosative stress following nitrate ingestion in the human upper gastrointestinal tract"]}]}],"canonical_facts":{"dc:creator":["Winter, Jack Westwood"],"dc:date":["2008"],"dc:date.issued":["2008"],"dc:description.abstract":["Nitrate ingestion leads to high luminal concentrations of nitric oxide being generated where saliva meets gastric acid. Nitric oxide generates N-nitrosative stress on reacting with oxygen at neutral pH. We aimed to ascertain if luminal nitric oxide exerts nitrosative stress in the upper GI tract, and to assess the influence of acid reflux. We utilised a specialised silicone tube as an epithelial model, inserting it into the upper gastro-intestinal tract of humans. Healthy volunteers were studied with and without ingestion of 15N enriched nitrate and Barrett’s oesophagus patients with and without stimulation of reflux. In volunteers, nitrate ingestion resulted in significantly higher concentrations of N-nitrosomorpholine in the tube sections exposed to acid. In Barrett’s patients, generation of N-nitrosomorpholine shifted proximally, with most nitrosative stress occurring within the oesophagus during reflux episodes. This chemistry may be harmful to patients with erosive esophagitis whose epithelium will be more sensitive to chemical mutagenesis."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://theses.gla.ac.uk/207/1/2008WinterMD.pdf"],"dc:language":["en"],"dc:publisher.institution":["University of Glasgow"],"dc:relation.isreferencedby":["https://theses.gla.ac.uk/207/"],"dc:relation.isreferencedby.uri":["https://gla.on.worldcat.org/oclc/244461558"],"dc:subject":["R Medicine (General)"],"dc:title":["Studies of in situ nitrosative stress following nitrate ingestion in the human upper gastrointestinal tract"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["MD"]},"updated_at":"2026-07-24T02:23:14Z"}