Biodiversity of phytoparasitic nematodes associated with Musaceae and fruit crops in Colombia
type: CreativeWork
description: Wide distributions of economically important plant-parasitic nematodes belonging to the genera Meloidogyne, Pratylenchus and Radopholus have been recorded in plantain and fruit crops in Colombia. Despite of significant economic losses, taxonomic research on these taxa in Colombia is limited. This thesis focuses mainly on two composition components of biodiversity (species and genes) as a contribution to the analyses of biodiversity of economically important plant-parasitic nematodes in Colombia. Samples were taken from 16 different regions of the country. Identification, characterization, and description of known and new nematode species should be based on a combination of as many data as possible, using different approaches. Morphometrical and morphological studies were based on LM and SEM observations, combined with other taxonomical methods such as analyses of proteins and use of species-specific primers (only for Meloidogyne populations), molecular analyses of sequences and phylogenetic analyses. In the current study, morphometric and/or morphological features allowed to separate populations into small species groups as a first step towards identification. Hereby, features of perineal pattern of females or head shape of males in Meloidogyne; head annulation number in Pratylenchus and tail length of Radopholus were the most helpful features at species level and reduced the number of species to be taken into account. From the combination of these analyses it turned out that several species ocurred together in the field. Thus, purification of populations is recommended as a second step in the identification process. In this study, only three Meloidogyne “populations” could be successfully purified. However, to overcome the mixed species problem for all other species, Meloidogyne identification was based on a single young egg-laying female (protein analysis) and DNA (for species-specific PCR and sequence analyses) was always extracted from a single juvenile. The hig…
abstract: Wide distributions of economically important plant-parasitic nematodes belonging to the genera Meloidogyne, Pratylenchus and Radopholus have been recorded in plantain and fruit crops in Colombia. Despite of significant economic losses, taxonomic research on these taxa in Colombia is limited. This thesis focuses mainly on two composition components of biodiversity (species and genes) as a contribution to the analyses of biodiversity of economically important plant-parasitic nematodes in Colombia. Samples were taken from 16 different regions of the country. Identification, characterization, and description of known and new nematode species should be based on a combination of as many data as possible, using different approaches. Morphometrical and morphological studies were based on LM and SEM observations, combined with other taxonomical methods such as analyses of proteins and use of species-specific primers (only for Meloidogyne populations), molecular analyses of sequences and phylogenetic analyses. In the current study, morphometric and/or morphological features allowed to separate populations into small species groups as a first step towards identification. Hereby, features of perineal pattern of females or head shape of males in Meloidogyne; head annulation number in Pratylenchus and tail length of Radopholus were the most helpful features at species level and reduced the number of species to be taken into account. From the combination of these analyses it turned out that several species ocurred together in the field. Thus, purification of populations is recommended as a second step in the identification process. In this study, only three Meloidogyne “populations” could be successfully purified. However, to overcome the mixed species problem for all other species, Meloidogyne identification was based on a single young egg-laying female (protein analysis) and DNA (for species-specific PCR and sequence analyses) was always extracted from a single juvenile. The hig…
author: Munera Uribe, G
language: und
date published: 2008
date modified: 2026-07-24T02:23:05Z
provider: Ghent University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ghent University

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property ID: Handle
value: http://hdl.handle.net/1854/LU-468437