{"id":{"repo_id":"gatech","oai_identifier":"oai:repository.gatech.edu:1853/81748"},"canonical_url":"https://search.dev.ndltd.org/etd/gatech/oai:repository.gatech.edu:1853/81748","repository":{"repo_id":"gatech","name":"Georgia Tech","base_url":"https://repository.gatech.edu/server/oai/request"},"display":{"title":"Investigating Poloidal Bias in DNA Minicircles and Overhang influence on DNA Dehybridization","abstract":"DNA is a dynamic molecule that undergoes constant physical manipulation by proteins. For instance, it experiences extreme bending when packaged into nucleosomes and undergoes strand separation (dehybridization) during transcription. Single-molecule assays offer a powerful approach to uncover the behavior of DNA during these processes. This dissertation presents two investigations utilizing single-molecule techniques to explore the anisotropic bending of DNA and the effects of single stranded overhangs on DNA dehybridization kinetics. The first investigation examines the extreme bending mechanics of DNA by directly probing the anisotropic nature of DNA minicircles through the detection of poloidal bias. We designed an assay using atomic force microscopy to map the orientation of these minicircles. By precisely monitoring the positions of protein-bound markers, we directly visualized poloidal orientation and successfully demonstrated a sequence-dependent poloidal bias in 105 bp minicircles. Our findings were further corroborated by coarse-grained simulations. The second investigation probes the effects of overhangs on the dehybridization kinetics of a DNA probe. Using single-molecule fluorescence resonance energy transfer, we found that the terminal base of the overhang can dictate dehybridization via base stacking. Furthermore, we identified a length-dependent relationship between the overhang and duplex stability, showing that shorter overhangs suppress dehybridization. Finally, we also found that when the overhang can form a base pair with itself, the overall stability of the bound DNA probe is significantly enhanced.","abstract_html":"DNA is a dynamic molecule that undergoes constant physical manipulation by proteins. For instance, it experiences extreme bending when packaged into nucleosomes and undergoes strand separation (dehybridization) during transcription. Single-molecule assays offer a powerful approach to uncover the behavior of DNA during these processes. This dissertation presents two investigations utilizing single-molecule techniques to explore the anisotropic bending of DNA and the effects of single stranded overhangs on DNA dehybridization kinetics. The first investigation examines the extreme bending mechanics of DNA by directly probing the anisotropic nature of DNA minicircles through the detection of poloidal bias. We designed an assay using atomic force microscopy to map the orientation of these minicircles. By precisely monitoring the positions of protein-bound markers, we directly visualized poloidal orientation and successfully demonstrated a sequence-dependent poloidal bias in 105 bp minicircles. Our findings were further corroborated by coarse-grained simulations. The second investigation probes the effects of overhangs on the dehybridization kinetics of a DNA probe. Using single-molecule fluorescence resonance energy transfer, we found that the terminal base of the overhang can dictate dehybridization via base stacking. Furthermore, we identified a length-dependent relationship between the overhang and duplex stability, showing that shorter overhangs suppress dehybridization. Finally, we also found that when the overhang can form a base pair with itself, the overall stability of the bound DNA probe is significantly enhanced.","abstract_has_math":false,"creators":["Lemos, Tony"],"institution":"Georgia Institute of Technology","degree_name":"Physics, PhD","degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Kim, Harold D."],"committee_chairs":[],"committee_members":["Gumbart, JC","Yunker, Peter","Rocklin, D. Zeb","Storici, Francesca"],"year":2026,"date_issued":"2026-05","date_published":"2026-05","updated_at":"2026-07-27T19:50:58Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1853/81748","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Kim, Harold D."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Gumbart, JC","Yunker, Peter","Rocklin, D. 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For instance, it experiences extreme bending when packaged into nucleosomes and undergoes strand separation (dehybridization) during transcription. Single-molecule assays offer a powerful approach to uncover the behavior of DNA during these processes. This dissertation presents two investigations utilizing single-molecule techniques to explore the anisotropic bending of DNA and the effects of single stranded overhangs on DNA dehybridization kinetics. The first investigation examines the extreme bending mechanics of DNA by directly probing the anisotropic nature of DNA minicircles through the detection of poloidal bias. We designed an assay using atomic force microscopy to map the orientation of these minicircles. By precisely monitoring the positions of protein-bound markers, we directly visualized poloidal orientation and successfully demonstrated a sequence-dependent poloidal bias in 105 bp minicircles. Our findings were further corroborated by coarse-grained simulations. The second investigation probes the effects of overhangs on the dehybridization kinetics of a DNA probe. Using single-molecule fluorescence resonance energy transfer, we found that the terminal base of the overhang can dictate dehybridization via base stacking. Furthermore, we identified a length-dependent relationship between the overhang and duplex stability, showing that shorter overhangs suppress dehybridization. Finally, we also found that when the overhang can form a base pair with itself, the overall stability of the bound DNA probe is significantly enhanced."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Investigating Poloidal Bias in DNA Minicircles and Overhang influence on DNA Dehybridization"]}]}],"canonical_facts":{"dc:contributor.advisor":["Kim, Harold D."],"dc:contributor.committeemember":["Gumbart, JC","Yunker, Peter","Rocklin, D. Zeb","Storici, Francesca"],"dc:creator":["Lemos, Tony"],"dc:date.accessioned":["2026-05-29T14:13:21Z"],"dc:date.available":["2026-05-29T14:13:21Z"],"dc:date.issued":["2026-05"],"dc:description.abstract":["DNA is a dynamic molecule that undergoes constant physical manipulation by proteins. For instance, it experiences extreme bending when packaged into nucleosomes and undergoes strand separation (dehybridization) during transcription. Single-molecule assays offer a powerful approach to uncover the behavior of DNA during these processes. This dissertation presents two investigations utilizing single-molecule techniques to explore the anisotropic bending of DNA and the effects of single stranded overhangs on DNA dehybridization kinetics. The first investigation examines the extreme bending mechanics of DNA by directly probing the anisotropic nature of DNA minicircles through the detection of poloidal bias. We designed an assay using atomic force microscopy to map the orientation of these minicircles. By precisely monitoring the positions of protein-bound markers, we directly visualized poloidal orientation and successfully demonstrated a sequence-dependent poloidal bias in 105 bp minicircles. Our findings were further corroborated by coarse-grained simulations. The second investigation probes the effects of overhangs on the dehybridization kinetics of a DNA probe. Using single-molecule fluorescence resonance energy transfer, we found that the terminal base of the overhang can dictate dehybridization via base stacking. Furthermore, we identified a length-dependent relationship between the overhang and duplex stability, showing that shorter overhangs suppress dehybridization. Finally, we also found that when the overhang can form a base pair with itself, the overall stability of the bound DNA probe is significantly enhanced."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/1853/81748"],"dc:title":["Investigating Poloidal Bias in DNA Minicircles and Overhang influence on DNA Dehybridization"],"dc:type":["Text"],"thesis:degree_name":["Physics, PhD"],"thesis:institution_name":["Georgia Institute of Technology"]},"updated_at":"2026-07-27T19:50:58Z"}