{"id":{"repo_id":"gatech","oai_identifier":"oai:repository.gatech.edu:1853/80050"},"canonical_url":"https://search.dev.ndltd.org/etd/gatech/oai:repository.gatech.edu:1853/80050","repository":{"repo_id":"gatech","name":"Georgia Tech","base_url":"https://repository.gatech.edu/server/oai/request"},"display":{"title":"Biocapacitance Sensors for In-Line Viable Cell Density Monitoring in Complex Bioreactors","abstract":"The precise monitoring of Viable Cell Density (VCD) in bioreactors is crucial for optimizing bioprocesses, improving yield, and maintaining product quality. VCD can be effectively indicated by biocapacitance, a capacitance associated with the viable cell membrane, which can be detected through Impedance/Dielectric Spectroscopy (IS/DS) The advancements of high-performance computing and Artificial Intelligence (AI) in recent decades have expedited the measuring and analysis of IS/DS enabling real-time monitoring of biocapacitance. In-line biocapacitance sensors has been widely applied for VCD monitoring in lab-scale adherent cell cultures and in high-throughput suspension cell cultures. However, this promising technology has not been effectively applied to high-throughput adherent cell cultures and lab-scale suspension cell cultures due to the complexity of the bioreactors. The Hollow Fiber Bioreactor (HFBR) is a high-efficiency bioreactor for high-throughput adherent cell culture thanks to its high surface-to-volume ratio. However, the packed interior of the HFBR presents unique challenges in achieving in-line biocapacitance sensing. This dissertation presents the development and characterization of biocapacitance sensors tailored for HFBRs. The study encompasses theoretical analysis, modeling, design, fabrication, and data analysis to create and optimize these sensors. The technology developed in this study is the first to achieve direct in-line VCD monitoring for high-throughput adherent cell culture. Key findings demonstrate that the sensors exhibit high sensitivity and potential to achieve spatial resolution, effectively monitoring VCD in real-time. Comprehensive data analysis and theoretical insights provides deeper understanding and optimization of sensor performance. Additionally, the work includes the development and assessment of two other biocapacitance sensors for lab-scale suspension cell cultures, which are also insufficiently addressed in current market and research. This work highlights the critical role of sensor technology, theoretical analysis, and data processing in advancing bioreactor systems.","abstract_html":"The precise monitoring of Viable Cell Density (VCD) in bioreactors is crucial for optimizing bioprocesses, improving yield, and maintaining product quality. VCD can be effectively indicated by biocapacitance, a capacitance associated with the viable cell membrane, which can be detected through Impedance/Dielectric Spectroscopy (IS/DS) The advancements of high-performance computing and Artificial Intelligence (AI) in recent decades have expedited the measuring and analysis of IS/DS enabling real-time monitoring of biocapacitance. In-line biocapacitance sensors has been widely applied for VCD monitoring in lab-scale adherent cell cultures and in high-throughput suspension cell cultures. However, this promising technology has not been effectively applied to high-throughput adherent cell cultures and lab-scale suspension cell cultures due to the complexity of the bioreactors. The Hollow Fiber Bioreactor (HFBR) is a high-efficiency bioreactor for high-throughput adherent cell culture thanks to its high surface-to-volume ratio. However, the packed interior of the HFBR presents unique challenges in achieving in-line biocapacitance sensing. This dissertation presents the development and characterization of biocapacitance sensors tailored for HFBRs. The study encompasses theoretical analysis, modeling, design, fabrication, and data analysis to create and optimize these sensors. The technology developed in this study is the first to achieve direct in-line VCD monitoring for high-throughput adherent cell culture. Key findings demonstrate that the sensors exhibit high sensitivity and potential to achieve spatial resolution, effectively monitoring VCD in real-time. Comprehensive data analysis and theoretical insights provides deeper understanding and optimization of sensor performance. Additionally, the work includes the development and assessment of two other biocapacitance sensors for lab-scale suspension cell cultures, which are also insufficiently addressed in current market and research. This work highlights the critical role of sensor technology, theoretical analysis, and data processing in advancing bioreactor systems.","abstract_has_math":false,"creators":["Liu, Zhaonan"],"institution":"Georgia Institute of Technology","degree_name":null,"degree_level":"Doctoral","degree_discipline":null,"degree_department":"Materials Science and Engineering","school":null,"contributors":[],"advisors":["Zhang, Chuck","Wang, Ben"],"committee_chairs":[],"committee_members":["Gerhardt, Rosario","Yao, Donggang","Wang, Kan"],"year":2024,"date_issued":"2024-08-20","date_published":"2024-08-20","updated_at":"2026-07-27T19:49:57Z","subjects":["Impedance spectroscopy","Biocapacitance","Viable cell density","Hollow fiber bioreactor"],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1853/80050","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Zhang, Chuck","Wang, Ben"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Gerhardt, Rosario","Yao, Donggang","Wang, Kan"]},{"key":"dc:contributor.department","label":"Department","values":["Materials Science and Engineering"]},{"key":"dc:creator","label":"Author","values":["Liu, Zhaonan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2026-01-20T15:00:00Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2026-01-20T15:00:00Z"]},{"key":"dc:date.issued","label":"Date","values":["2024-08-20"]},{"key":"dc:publisher","label":"Institution","values":["Georgia Institute of Technology"]},{"key":"dc:type","label":"Dc Type","values":["Text"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Impedance spectroscopy","Biocapacitance","Viable cell density","Hollow fiber bioreactor"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1853/80050"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The precise monitoring of Viable Cell Density (VCD) in bioreactors is crucial for optimizing bioprocesses, improving yield, and maintaining product quality. VCD can be effectively indicated by biocapacitance, a capacitance associated with the viable cell membrane, which can be detected through Impedance/Dielectric Spectroscopy (IS/DS) The advancements of high-performance computing and Artificial Intelligence (AI) in recent decades have expedited the measuring and analysis of IS/DS enabling real-time monitoring of biocapacitance. In-line biocapacitance sensors has been widely applied for VCD monitoring in lab-scale adherent cell cultures and in high-throughput suspension cell cultures. However, this promising technology has not been effectively applied to high-throughput adherent cell cultures and lab-scale suspension cell cultures due to the complexity of the bioreactors. The Hollow Fiber Bioreactor (HFBR) is a high-efficiency bioreactor for high-throughput adherent cell culture thanks to its high surface-to-volume ratio. However, the packed interior of the HFBR presents unique challenges in achieving in-line biocapacitance sensing. This dissertation presents the development and characterization of biocapacitance sensors tailored for HFBRs. The study encompasses theoretical analysis, modeling, design, fabrication, and data analysis to create and optimize these sensors. The technology developed in this study is the first to achieve direct in-line VCD monitoring for high-throughput adherent cell culture. Key findings demonstrate that the sensors exhibit high sensitivity and potential to achieve spatial resolution, effectively monitoring VCD in real-time. Comprehensive data analysis and theoretical insights provides deeper understanding and optimization of sensor performance. Additionally, the work includes the development and assessment of two other biocapacitance sensors for lab-scale suspension cell cultures, which are also insufficiently addressed in current market and research. This work highlights the critical role of sensor technology, theoretical analysis, and data processing in advancing bioreactor systems."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph.D."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Biocapacitance Sensors for In-Line Viable Cell Density Monitoring in Complex Bioreactors"]}]}],"canonical_facts":{"dc:contributor.advisor":["Zhang, Chuck","Wang, Ben"],"dc:contributor.committeemember":["Gerhardt, Rosario","Yao, Donggang","Wang, Kan"],"dc:contributor.department":["Materials Science and Engineering"],"dc:creator":["Liu, Zhaonan"],"dc:date.accessioned":["2026-01-20T15:00:00Z"],"dc:date.available":["2026-01-20T15:00:00Z"],"dc:date.issued":["2024-08-20"],"dc:description.abstract":["The precise monitoring of Viable Cell Density (VCD) in bioreactors is crucial for optimizing bioprocesses, improving yield, and maintaining product quality. VCD can be effectively indicated by biocapacitance, a capacitance associated with the viable cell membrane, which can be detected through Impedance/Dielectric Spectroscopy (IS/DS) The advancements of high-performance computing and Artificial Intelligence (AI) in recent decades have expedited the measuring and analysis of IS/DS enabling real-time monitoring of biocapacitance. In-line biocapacitance sensors has been widely applied for VCD monitoring in lab-scale adherent cell cultures and in high-throughput suspension cell cultures. However, this promising technology has not been effectively applied to high-throughput adherent cell cultures and lab-scale suspension cell cultures due to the complexity of the bioreactors. The Hollow Fiber Bioreactor (HFBR) is a high-efficiency bioreactor for high-throughput adherent cell culture thanks to its high surface-to-volume ratio. However, the packed interior of the HFBR presents unique challenges in achieving in-line biocapacitance sensing. This dissertation presents the development and characterization of biocapacitance sensors tailored for HFBRs. The study encompasses theoretical analysis, modeling, design, fabrication, and data analysis to create and optimize these sensors. The technology developed in this study is the first to achieve direct in-line VCD monitoring for high-throughput adherent cell culture. Key findings demonstrate that the sensors exhibit high sensitivity and potential to achieve spatial resolution, effectively monitoring VCD in real-time. Comprehensive data analysis and theoretical insights provides deeper understanding and optimization of sensor performance. Additionally, the work includes the development and assessment of two other biocapacitance sensors for lab-scale suspension cell cultures, which are also insufficiently addressed in current market and research. This work highlights the critical role of sensor technology, theoretical analysis, and data processing in advancing bioreactor systems."],"dc:description.degree":["Ph.D."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/1853/80050"],"dc:language.iso":["en_US"],"dc:publisher":["Georgia Institute of Technology"],"dc:subject":["Impedance spectroscopy","Biocapacitance","Viable cell density","Hollow fiber bioreactor"],"dc:title":["Biocapacitance Sensors for In-Line Viable Cell Density Monitoring in Complex Bioreactors"],"dc:type":["Text"],"thesis:degree_level":["Doctoral"]},"updated_at":"2026-07-27T19:49:57Z"}