{"id":{"repo_id":"freiburg-diss","oai_identifier":"oai:freidok.uni-freiburg.de:250"},"canonical_url":"https://search.dev.ndltd.org/etd/freiburg-diss/oai:freidok.uni-freiburg.de:250","repository":{"repo_id":"freiburg-diss","name":"University of Freiburg","base_url":"https://freidok.uni-freiburg.de/oai/oai2.php"},"display":{"title":"Isolation and characterization of primitive hematopoietic cells","abstract":"Side-population (SP)cells are selected based on the rapid efflux of the fluorescent DNA-binding dye Hoechst 33342. Murine BM SP cells had long-term repopulating activity and proved negative for the antigen CD34. Such SP cells were detected in rats, pigs, monkeys and human cell sources. Within SP cells from human umbilical CB, CD7+/D34- lymphoid progenitor cells were recently described. SP cells from other human hematopoietic cell sources, such as BM from normal donors and patients with hematological malignancies, or mobilized PB from donors and patients, however, have not been characterized extensively. We therefore sought to characterize SP cells from these human cell sources, analyzed their CD34 expression, CFU-, LTC-IC potential. We detected murine SP with a median frequency of 0.03 When co-staining with verapamil was performed, the frequency of SP cells significantly decreased. There was an increase of CFUs yielded from murine SP cells as compared to unfractionated cells with a median enrichment of 52- fold. The frequencies of human SP cells were highest in AP (0.03), followed by CB (0.02) and lowest in BM. Most human SP cells were CD34- and the majority of CD34- cells were CD34- CD38-. In contrast to murine SP cells, the CFU potential of human SP cells was not increased as compared to unfractionated cells, but after short-term suspension culture, high CFU numbers were yielded. LTC-ICs were increased by a median of 49-fold from SP as compared to unfractionated cells. The highest increase was observed from donor AP SP (median 184-fold), followed by CB SP cells (53-fold), and lowest from patient AP and donor BM. Our data demonstrate that primitive hematopoietic cells can be isolated via Hoechst staining. Ongoing experiments subdividing the human Lin- SP population into CD34+ and CD34- cells will allow us to compare their LTC-IC potential and in vivo repopulating capacity, thereby aiding in the discussion whether HSC are CD34+ or CD34-.","abstract_html":"Side-population (SP)cells are selected based on the rapid efflux of the fluorescent DNA-binding dye Hoechst 33342. Murine BM SP cells had long-term repopulating activity and proved negative for the antigen CD34. Such SP cells were detected in rats, pigs, monkeys and human cell sources. Within SP cells from human umbilical CB, CD7+/D34- lymphoid progenitor cells were recently described. SP cells from other human hematopoietic cell sources, such as BM from normal donors and patients with hematological malignancies, or mobilized PB from donors and patients, however, have not been characterized extensively. We therefore sought to characterize SP cells from these human cell sources, analyzed their CD34 expression, CFU-, LTC-IC potential. We detected murine SP with a median frequency of 0.03 When co-staining with verapamil was performed, the frequency of SP cells significantly decreased. There was an increase of CFUs yielded from murine SP cells as compared to unfractionated cells with a median enrichment of 52- fold. The frequencies of human SP cells were highest in AP (0.03), followed by CB (0.02) and lowest in BM. Most human SP cells were CD34- and the majority of CD34- cells were CD34- CD38-. In contrast to murine SP cells, the CFU potential of human SP cells was not increased as compared to unfractionated cells, but after short-term suspension culture, high CFU numbers were yielded. LTC-ICs were increased by a median of 49-fold from SP as compared to unfractionated cells. The highest increase was observed from donor AP SP (median 184-fold), followed by CB SP cells (53-fold), and lowest from patient AP and donor BM. Our data demonstrate that primitive hematopoietic cells can be isolated via Hoechst staining. Ongoing experiments subdividing the human Lin- SP population into CD34+ and CD34- cells will allow us to compare their LTC-IC potential and in vivo repopulating capacity, thereby aiding in the discussion whether HSC are CD34+ or CD34-.","abstract_has_math":false,"creators":["Guo, Yalin"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Lübbert, Michael"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T02:21:35Z","subjects":["sorting"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://freidok.uni-freiburg.de/data/250","outbound_label":"Repository record","outbound_source":"source_url"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Lübbert, Michael"]},{"key":"dc:creator","label":"Author","values":["Guo, Yalin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:type","label":"Dc Type","values":["DoctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["sorting"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Side-population (SP)cells are selected based on the rapid efflux of the fluorescent DNA-binding dye Hoechst 33342. Murine BM SP cells had long-term repopulating activity and proved negative for the antigen CD34. Such SP cells were detected in rats, pigs, monkeys and human cell sources. Within SP cells from human umbilical CB, CD7+/D34- lymphoid progenitor cells were recently described. SP cells from other human hematopoietic cell sources, such as BM from normal donors and patients with hematological malignancies, or mobilized PB from donors and patients, however, have not been characterized extensively. We therefore sought to characterize SP cells from these human cell sources, analyzed their CD34 expression, CFU-, LTC-IC potential. We detected murine SP with a median frequency of 0.03 When co-staining with verapamil was performed, the frequency of SP cells significantly decreased. There was an increase of CFUs yielded from murine SP cells as compared to unfractionated cells with a median enrichment of 52- fold. The frequencies of human SP cells were highest in AP (0.03), followed by CB (0.02) and lowest in BM. Most human SP cells were CD34- and the majority of CD34- cells were CD34- CD38-. In contrast to murine SP cells, the CFU potential of human SP cells was not increased as compared to unfractionated cells, but after short-term suspension culture, high CFU numbers were yielded. LTC-ICs were increased by a median of 49-fold from SP as compared to unfractionated cells. The highest increase was observed from donor AP SP (median 184-fold), followed by CB SP cells (53-fold), and lowest from patient AP and donor BM. Our data demonstrate that primitive hematopoietic cells can be isolated via Hoechst staining. Ongoing experiments subdividing the human Lin- SP population into CD34+ and CD34- cells will allow us to compare their LTC-IC potential and in vivo repopulating capacity, thereby aiding in the discussion whether HSC are CD34+ or CD34-."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Isolation and characterization of primitive hematopoietic cells","Charakterisierung hämatopoetischer Stammzellen nach Sortierung mit dem Vitalfarbstoff Hoechst von murinen und humanen Zellproben"]}]}],"canonical_facts":{"dc:contributor":["Lübbert, Michael"],"dc:creator":["Guo, Yalin"],"dc:description.abstract":["Side-population (SP)cells are selected based on the rapid efflux of the fluorescent DNA-binding dye Hoechst 33342. Murine BM SP cells had long-term repopulating activity and proved negative for the antigen CD34. Such SP cells were detected in rats, pigs, monkeys and human cell sources. Within SP cells from human umbilical CB, CD7+/D34- lymphoid progenitor cells were recently described. SP cells from other human hematopoietic cell sources, such as BM from normal donors and patients with hematological malignancies, or mobilized PB from donors and patients, however, have not been characterized extensively. We therefore sought to characterize SP cells from these human cell sources, analyzed their CD34 expression, CFU-, LTC-IC potential. We detected murine SP with a median frequency of 0.03 When co-staining with verapamil was performed, the frequency of SP cells significantly decreased. There was an increase of CFUs yielded from murine SP cells as compared to unfractionated cells with a median enrichment of 52- fold. The frequencies of human SP cells were highest in AP (0.03), followed by CB (0.02) and lowest in BM. Most human SP cells were CD34- and the majority of CD34- cells were CD34- CD38-. In contrast to murine SP cells, the CFU potential of human SP cells was not increased as compared to unfractionated cells, but after short-term suspension culture, high CFU numbers were yielded. LTC-ICs were increased by a median of 49-fold from SP as compared to unfractionated cells. The highest increase was observed from donor AP SP (median 184-fold), followed by CB SP cells (53-fold), and lowest from patient AP and donor BM. Our data demonstrate that primitive hematopoietic cells can be isolated via Hoechst staining. Ongoing experiments subdividing the human Lin- SP population into CD34+ and CD34- cells will allow us to compare their LTC-IC potential and in vivo repopulating capacity, thereby aiding in the discussion whether HSC are CD34+ or CD34-."],"dc:format.medium":["application/pdf"],"dc:subject":["sorting"],"dc:title":["Isolation and characterization of primitive hematopoietic cells","Charakterisierung hämatopoetischer Stammzellen nach Sortierung mit dem Vitalfarbstoff Hoechst von murinen und humanen Zellproben"],"dc:type":["DoctoralThesis"]},"updated_at":"2026-07-24T02:21:35Z"}