{"id":{"repo_id":"freiburg-diss","oai_identifier":"oai:freidok.uni-freiburg.de:1890"},"canonical_url":"https://search.dev.ndltd.org/etd/freiburg-diss/oai:freidok.uni-freiburg.de:1890","repository":{"repo_id":"freiburg-diss","name":"University of Freiburg","base_url":"https://freidok.uni-freiburg.de/oai/oai2.php"},"display":{"title":"Reoxygenation of hypoxic coronary smooth muscle cells amplifies growth-retarding effects of ionizing irradiation","abstract":"Hypoxic coronary smooth muscle cells (HCSMCs) are potential targets for brachytherapy to prevent restenosis after percutaneous transluminal angioplasty. It is unclear whether gene expression, apoptosis rate and production of reactive oxygen species of these cells undergoing alpha-irradiation are changed by reoxygenation. <br>Hypoxic and reoxygenated HCSMCs were irradiated with alpha-radiation at single doses of 4, 8 and 16 Gy using a 60Co-source. Vascular endothelial growth factor (VEGF) gene expression was dramatically suppressed in reoxygenated versus hypoxic HCSMCs independent of the radiation dose. Likewise, hypoxia induced factor 1alpha (HIF-1alpha)expression was suppressed in reoxygenated versus hypoxic HCSMCs. Production of reactive oxygen species and apoptosis rate after irradiation were increased significantly in reoxygenated versus hypoxic HCSMCs. <br>Reoxygenation of hypoxic HCSMCs markedly amplifies growth-retarding effects of ionizing irradiation via induction of HIF-1-alpha. On the basis of these findings, oxygenating radiosensitizers should be analysed with regard to suitablility for coronary brachytherapy to prevent restenosis.","abstract_html":"Hypoxic coronary smooth muscle cells (HCSMCs) are potential targets for brachytherapy to prevent restenosis after percutaneous transluminal angioplasty. It is unclear whether gene expression, apoptosis rate and production of reactive oxygen species of these cells undergoing alpha-irradiation are changed by reoxygenation. &lt;br&gt;Hypoxic and reoxygenated HCSMCs were irradiated with alpha-radiation at single doses of 4, 8 and 16 Gy using a 60Co-source. Vascular endothelial growth factor (VEGF) gene expression was dramatically suppressed in reoxygenated versus hypoxic HCSMCs independent of the radiation dose. Likewise, hypoxia induced factor 1alpha (HIF-1alpha)expression was suppressed in reoxygenated versus hypoxic HCSMCs. Production of reactive oxygen species and apoptosis rate after irradiation were increased significantly in reoxygenated versus hypoxic HCSMCs. &lt;br&gt;Reoxygenation of hypoxic HCSMCs markedly amplifies growth-retarding effects of ionizing irradiation via induction of HIF-1-alpha. On the basis of these findings, oxygenating radiosensitizers should be analysed with regard to suitablility for coronary brachytherapy to prevent restenosis.","abstract_has_math":false,"creators":["Arab, Amina"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Bode, Christoph"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T02:22:33Z","subjects":["hypoxia","radiotherapy","restenosis"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://freidok.uni-freiburg.de/data/1890","outbound_label":"Repository record","outbound_source":"source_url"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bode, Christoph"]},{"key":"dc:creator","label":"Author","values":["Arab, Amina"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:type","label":"Dc Type","values":["DoctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["hypoxia","radiotherapy","restenosis"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Hypoxic coronary smooth muscle cells (HCSMCs) are potential targets for brachytherapy to prevent restenosis after percutaneous transluminal angioplasty. It is unclear whether gene expression, apoptosis rate and production of reactive oxygen species of these cells undergoing alpha-irradiation are changed by reoxygenation. <br>Hypoxic and reoxygenated HCSMCs were irradiated with alpha-radiation at single doses of 4, 8 and 16 Gy using a 60Co-source. Vascular endothelial growth factor (VEGF) gene expression was dramatically suppressed in reoxygenated versus hypoxic HCSMCs independent of the radiation dose. Likewise, hypoxia induced factor 1alpha (HIF-1alpha)expression was suppressed in reoxygenated versus hypoxic HCSMCs. Production of reactive oxygen species and apoptosis rate after irradiation were increased significantly in reoxygenated versus hypoxic HCSMCs. <br>Reoxygenation of hypoxic HCSMCs markedly amplifies growth-retarding effects of ionizing irradiation via induction of HIF-1-alpha. On the basis of these findings, oxygenating radiosensitizers should be analysed with regard to suitablility for coronary brachytherapy to prevent restenosis.","Glatte Muskelzellen (GMZ) der koronaren Gefäßwand sind nach Angaben aus der Literatur die möglichen Zielzellen der Strahlentherapie zur Reduktion der Restenose nach perkutaner transluminaler Koronarangioplastie. Es ist unklar, wie sich die Genexpression, Apoptoserate und die Produktion freien Sauerstoffradikalen in GMZ nach Behandlung mit alpha-Strahlung unter Reoxygenierung im Vergleich zur Hypoxie verändert. <br>Hypoxische und reoxygenierte GMZ wurden mit Einzeldosen von 4, 8 und 16 Gy aus einer 60Cobald-Quelle bestrahlt. Die Genexpression des vaskulär endothelialen Wachstumsfaktors (VEGF) war in reoxygenierten GMZ verglichen mit hypoxischen GMZ stark suppremiert und zwar weitgehend unabhängig von der applizierten Stahlendosis. Auch die Expression des Hypoxie-induzierten Faktors 1-alpha (HIF-1-alpha Gens war in reoxygenierten Zellen signifikant niedriger als in hypoxischen Zellen und ebenfalls weitgehend unabhängig von der applizierten Stahlendosis. Die Produktion freier Sauerstoffradikale und die Apoptoserate dagegen waren nach Bestrahlung von reoxygenierten GMZ im Vergleich zu hypoxischen GMZ signifikant erhöht. <br>Wir folgern daraus, daß die Reoxygenierung hypoxischer GMZ den anti-proliferativen Effekt der Strahlentherapie verstärkt und die Induktion des HIF-1-alpha Gens eine zentrale Rolle bei dieser Verstärkung spielt. Daher sollten die Verfahren der Tumoroxygenierung in der Radioonkologie daraufhin untersucht werden, ob sie in der intrakoronaren Strahlentherapie zur Verhinderung der Restenose nach Angioplastie anwendbar sind."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Reoxygenation of hypoxic coronary smooth muscle cells amplifies growth-retarding effects of ionizing irradiation","Die Oxygenierung hypoxischer koronarer Muskelzellen verstärkt den anti-proliferativen Effekt von gamma-Strahlung"]}]}],"canonical_facts":{"dc:contributor":["Bode, Christoph"],"dc:creator":["Arab, Amina"],"dc:description.abstract":["Hypoxic coronary smooth muscle cells (HCSMCs) are potential targets for brachytherapy to prevent restenosis after percutaneous transluminal angioplasty. It is unclear whether gene expression, apoptosis rate and production of reactive oxygen species of these cells undergoing alpha-irradiation are changed by reoxygenation. <br>Hypoxic and reoxygenated HCSMCs were irradiated with alpha-radiation at single doses of 4, 8 and 16 Gy using a 60Co-source. Vascular endothelial growth factor (VEGF) gene expression was dramatically suppressed in reoxygenated versus hypoxic HCSMCs independent of the radiation dose. Likewise, hypoxia induced factor 1alpha (HIF-1alpha)expression was suppressed in reoxygenated versus hypoxic HCSMCs. Production of reactive oxygen species and apoptosis rate after irradiation were increased significantly in reoxygenated versus hypoxic HCSMCs. <br>Reoxygenation of hypoxic HCSMCs markedly amplifies growth-retarding effects of ionizing irradiation via induction of HIF-1-alpha. On the basis of these findings, oxygenating radiosensitizers should be analysed with regard to suitablility for coronary brachytherapy to prevent restenosis.","Glatte Muskelzellen (GMZ) der koronaren Gefäßwand sind nach Angaben aus der Literatur die möglichen Zielzellen der Strahlentherapie zur Reduktion der Restenose nach perkutaner transluminaler Koronarangioplastie. Es ist unklar, wie sich die Genexpression, Apoptoserate und die Produktion freien Sauerstoffradikalen in GMZ nach Behandlung mit alpha-Strahlung unter Reoxygenierung im Vergleich zur Hypoxie verändert. <br>Hypoxische und reoxygenierte GMZ wurden mit Einzeldosen von 4, 8 und 16 Gy aus einer 60Cobald-Quelle bestrahlt. Die Genexpression des vaskulär endothelialen Wachstumsfaktors (VEGF) war in reoxygenierten GMZ verglichen mit hypoxischen GMZ stark suppremiert und zwar weitgehend unabhängig von der applizierten Stahlendosis. Auch die Expression des Hypoxie-induzierten Faktors 1-alpha (HIF-1-alpha Gens war in reoxygenierten Zellen signifikant niedriger als in hypoxischen Zellen und ebenfalls weitgehend unabhängig von der applizierten Stahlendosis. Die Produktion freier Sauerstoffradikale und die Apoptoserate dagegen waren nach Bestrahlung von reoxygenierten GMZ im Vergleich zu hypoxischen GMZ signifikant erhöht. <br>Wir folgern daraus, daß die Reoxygenierung hypoxischer GMZ den anti-proliferativen Effekt der Strahlentherapie verstärkt und die Induktion des HIF-1-alpha Gens eine zentrale Rolle bei dieser Verstärkung spielt. Daher sollten die Verfahren der Tumoroxygenierung in der Radioonkologie daraufhin untersucht werden, ob sie in der intrakoronaren Strahlentherapie zur Verhinderung der Restenose nach Angioplastie anwendbar sind."],"dc:format.medium":["application/pdf"],"dc:subject":["hypoxia","radiotherapy","restenosis"],"dc:title":["Reoxygenation of hypoxic coronary smooth muscle cells amplifies growth-retarding effects of ionizing irradiation","Die Oxygenierung hypoxischer koronarer Muskelzellen verstärkt den anti-proliferativen Effekt von gamma-Strahlung"],"dc:type":["DoctoralThesis"]},"updated_at":"2026-07-24T02:22:33Z"}