Multi-view microscopy and multi-beam manipulation for high-resolution optical imaging
The work presented in this thesis tackles some of the challenges in optical conventional and confocal microscopy and opens the third dimension for biological experiments like 3D cell cultures. Two instruments were built that provide high and isotropic resolution. In the Multi Imaging AxisMicroscope (MIAM) four objective lenses are used to both image the sample and to manipulate it with optical forces. The Selective Plane Illumination Microscope (SPIM) is especially well suited for large biological samples and provides optical sectioning deep inside the specimen, efficient fluorescence excitation and reduced photo-bleaching. A technique for gentle sample preparation and manipulation is presented. Both instruments share the technique of multi-view combination to improve the overall image quality and the resolution in all directions.
optische Falle, Rotation, Probenpräparation, microscopy, confocal microscopy, image processing, optical trap, optical tweezers
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