{"id":{"repo_id":"freiburg-diss","oai_identifier":"oai:freidok.uni-freiburg.de:1533"},"canonical_url":"https://search.dev.ndltd.org/etd/freiburg-diss/oai:freidok.uni-freiburg.de:1533","repository":{"repo_id":"freiburg-diss","name":"University of Freiburg","base_url":"https://freidok.uni-freiburg.de/oai/oai2.php"},"display":{"title":"Multi-view microscopy and multi-beam manipulation for high-resolution optical imaging","abstract":"The work presented in this thesis tackles some of the challenges in optical conventional and confocal microscopy and opens the third dimension for biological experiments like 3D cell cultures. Two instruments were built that provide high and isotropic resolution. In the Multi Imaging AxisMicroscope (MIAM) four objective lenses are used to both image the sample and to manipulate it with optical forces. The Selective Plane Illumination Microscope (SPIM) is especially well suited for large biological samples and provides optical sectioning deep inside the specimen, efficient fluorescence excitation and reduced photo-bleaching. A technique for gentle sample preparation and manipulation is presented. Both instruments share the technique of multi-view combination to improve the overall image quality and the resolution in all directions.","abstract_html":"The work presented in this thesis tackles some of the challenges in optical conventional and confocal microscopy and opens the third dimension for biological experiments like 3D cell cultures. Two instruments were built that provide high and isotropic resolution. In the Multi Imaging AxisMicroscope (MIAM) four objective lenses are used to both image the sample and to manipulate it with optical forces. The Selective Plane Illumination Microscope (SPIM) is especially well suited for large biological samples and provides optical sectioning deep inside the specimen, efficient fluorescence excitation and reduced photo-bleaching. A technique for gentle sample preparation and manipulation is presented. Both instruments share the technique of multi-view combination to improve the overall image quality and the resolution in all directions.","abstract_has_math":false,"creators":["Huisken, Jan"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Weidemüller, Matthias"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T02:22:16Z","subjects":["optische Falle","Rotation","Probenpräparation","microscopy","confocal microscopy","image processing","optical trap","optical tweezers"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://freidok.uni-freiburg.de/data/1533","outbound_label":"Repository record","outbound_source":"source_url"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Weidemüller, Matthias"]},{"key":"dc:creator","label":"Author","values":["Huisken, Jan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:type","label":"Dc Type","values":["DoctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["optische Falle","Rotation","Probenpräparation","microscopy","confocal microscopy","image processing","optical trap","optical tweezers"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The work presented in this thesis tackles some of the challenges in optical conventional and confocal microscopy and opens the third dimension for biological experiments like 3D cell cultures. Two instruments were built that provide high and isotropic resolution. In the Multi Imaging AxisMicroscope (MIAM) four objective lenses are used to both image the sample and to manipulate it with optical forces. The Selective Plane Illumination Microscope (SPIM) is especially well suited for large biological samples and provides optical sectioning deep inside the specimen, efficient fluorescence excitation and reduced photo-bleaching. A technique for gentle sample preparation and manipulation is presented. Both instruments share the technique of multi-view combination to improve the overall image quality and the resolution in all directions.","Die vorliegende Arbeit adressiert einige Limitation konventioneller und konfokaler Mikroskopie und öffnet die dritte Dimension für biologische Experimente wie 3D-Zellkulturen. Die zwei Geräte, die entwickelt und gebaut wurden, liefern hohe und isotrope Auflösung. Im Multi Imaging Axis Microscope (MIAM) dienen vier Objektive zur Bildgebung und zur optischen Manipulation der Probe. Das Selective Plane Illumination Microscope (SPIM) ist besonders geeignet für ausgedehnte biologische Proben und liefert optische Sektionierung tief in der Probe, effiziente Fluoreszenz-Anregung und reduziertes Bleichen. Ein Verfahren wird beschrieben zur schonenden Probenpräparation und -manipulation. Beide Geräte nutzen die Technik der Kombination mehrerer Ansichten zur Verbesserung von Bildqualitat und Aufösung in alle Raumrichtungen."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Multi-view microscopy and multi-beam manipulation for high-resolution optical imaging","Multi-Winkel Mikroskopie und Multi-Strahl Manipulation für hochauflösende optische Bildgebung"]}]}],"canonical_facts":{"dc:contributor":["Weidemüller, Matthias"],"dc:creator":["Huisken, Jan"],"dc:description.abstract":["The work presented in this thesis tackles some of the challenges in optical conventional and confocal microscopy and opens the third dimension for biological experiments like 3D cell cultures. Two instruments were built that provide high and isotropic resolution. In the Multi Imaging AxisMicroscope (MIAM) four objective lenses are used to both image the sample and to manipulate it with optical forces. The Selective Plane Illumination Microscope (SPIM) is especially well suited for large biological samples and provides optical sectioning deep inside the specimen, efficient fluorescence excitation and reduced photo-bleaching. A technique for gentle sample preparation and manipulation is presented. Both instruments share the technique of multi-view combination to improve the overall image quality and the resolution in all directions.","Die vorliegende Arbeit adressiert einige Limitation konventioneller und konfokaler Mikroskopie und öffnet die dritte Dimension für biologische Experimente wie 3D-Zellkulturen. Die zwei Geräte, die entwickelt und gebaut wurden, liefern hohe und isotrope Auflösung. Im Multi Imaging Axis Microscope (MIAM) dienen vier Objektive zur Bildgebung und zur optischen Manipulation der Probe. Das Selective Plane Illumination Microscope (SPIM) ist besonders geeignet für ausgedehnte biologische Proben und liefert optische Sektionierung tief in der Probe, effiziente Fluoreszenz-Anregung und reduziertes Bleichen. Ein Verfahren wird beschrieben zur schonenden Probenpräparation und -manipulation. Beide Geräte nutzen die Technik der Kombination mehrerer Ansichten zur Verbesserung von Bildqualitat und Aufösung in alle Raumrichtungen."],"dc:format.medium":["application/pdf"],"dc:subject":["optische Falle","Rotation","Probenpräparation","microscopy","confocal microscopy","image processing","optical trap","optical tweezers"],"dc:title":["Multi-view microscopy and multi-beam manipulation for high-resolution optical imaging","Multi-Winkel Mikroskopie und Multi-Strahl Manipulation für hochauflösende optische Bildgebung"],"dc:type":["DoctoralThesis"]},"updated_at":"2026-07-24T02:22:16Z"}