{"id":{"repo_id":"freiburg-diss","oai_identifier":"oai:freidok.uni-freiburg.de:1463"},"canonical_url":"https://search.dev.ndltd.org/etd/freiburg-diss/oai:freidok.uni-freiburg.de:1463","repository":{"repo_id":"freiburg-diss","name":"University of Freiburg","base_url":"https://freidok.uni-freiburg.de/oai/oai2.php"},"display":{"title":"Influence of Oxygenated Perfluorocarbon (oxyPFC) on survival and free radical production of Ischemic Cardiomyocytes","abstract":"In the late 1980’s, several animal studies showed that oxyPFC given intravenously or via intracoronary route have the potential to reduce the size of myocardial infarctions. The exact mechanism by which this reduction occurred remained unclear until today. The subject of this experimental work was to elucidate the pathways of interaction of oxyPFC with hypoxic cardiomyocytes. The first mechanism we elucidated in this study was the effect of oxyPFC on mitochondrial respiration in a hypoxic state of the cardiomyocyte, and the second was to find out which role oxygen free radicals, especially superoxide, play after administration of oxyPFC. Several cellular biological methods were used to analyse mitochondrial respiration under the influence of oxyPFC. The results show that myocardial viability and ATP-production is increased after application of small doses of oxyPFC. The results further indicated that superoxide is up-regulated in an early phase after oxyPFC application, and down-regulated 6 hours after oxyPFC application. This experimental study clarified some of the physiological mechanisms by which oxyPFC reduces the infarct size. Our results suggest that oxyPFC may be useful as an adjunct therapy in myocardial infarction to increase survival of cardiomyocytes.","abstract_html":"In the late 1980’s, several animal studies showed that oxyPFC given intravenously or via intracoronary route have the potential to reduce the size of myocardial infarctions. The exact mechanism by which this reduction occurred remained unclear until today. The subject of this experimental work was to elucidate the pathways of interaction of oxyPFC with hypoxic cardiomyocytes. The first mechanism we elucidated in this study was the effect of oxyPFC on mitochondrial respiration in a hypoxic state of the cardiomyocyte, and the second was to find out which role oxygen free radicals, especially superoxide, play after administration of oxyPFC. Several cellular biological methods were used to analyse mitochondrial respiration under the influence of oxyPFC. The results show that myocardial viability and ATP-production is increased after application of small doses of oxyPFC. The results further indicated that superoxide is up-regulated in an early phase after oxyPFC application, and down-regulated 6 hours after oxyPFC application. This experimental study clarified some of the physiological mechanisms by which oxyPFC reduces the infarct size. Our results suggest that oxyPFC may be useful as an adjunct therapy in myocardial infarction to increase survival of cardiomyocytes.","abstract_has_math":false,"creators":["Wang, Jin"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Hehrlein, Christoph"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T02:22:16Z","subjects":["Ischemia","Oxygenation","Free Radical Production"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://freidok.uni-freiburg.de/data/1463","outbound_label":"Repository record","outbound_source":"source_url"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hehrlein, Christoph"]},{"key":"dc:creator","label":"Author","values":["Wang, Jin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:type","label":"Dc Type","values":["DoctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ischemia","Oxygenation","Free Radical Production"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In the late 1980’s, several animal studies showed that oxyPFC given intravenously or via intracoronary route have the potential to reduce the size of myocardial infarctions. The exact mechanism by which this reduction occurred remained unclear until today. The subject of this experimental work was to elucidate the pathways of interaction of oxyPFC with hypoxic cardiomyocytes. The first mechanism we elucidated in this study was the effect of oxyPFC on mitochondrial respiration in a hypoxic state of the cardiomyocyte, and the second was to find out which role oxygen free radicals, especially superoxide, play after administration of oxyPFC. Several cellular biological methods were used to analyse mitochondrial respiration under the influence of oxyPFC. The results show that myocardial viability and ATP-production is increased after application of small doses of oxyPFC. The results further indicated that superoxide is up-regulated in an early phase after oxyPFC application, and down-regulated 6 hours after oxyPFC application. This experimental study clarified some of the physiological mechanisms by which oxyPFC reduces the infarct size. Our results suggest that oxyPFC may be useful as an adjunct therapy in myocardial infarction to increase survival of cardiomyocytes.","Ende der 80er Jahre zeigten eine Reihe von tierexperimentellen Studien, dass die Ausdehnung eines Myokardinfarkts durch intravenöse oder intrakoronare Zugabe von oxigenierten Perfluorocarbonen (oxPFC) deutlich reduziert werden kann. Der Mechanismus dieser Reduktion der Infarktgrösse durch oxigeniertes Perfluorocarbon ist bis heute unklar geblieben. Es war Gegenstand dieser wissenschaftlichen Arbeit an humanen Herzmuskelzellen zu untersuchen, 1. welche Mechanismen im Sauerstoffstoffwechsel für das Überleben von Herzmuskelzellen in der Phase der Hypoxie bzw. Ischämie eine Rolle spielen und 2. aufzuzeigen, welches Wirkungsspektrum oxigeniertes Perfluorocarbon bei der Reoxigenierung von Herzmuskelzellen und deren Produktion von Sauerstoffradikalen, insbesondere der Superoxidproduktion besitzen. Mit mehreren zellbiologischen Methoden konnte gezeigt werden, dass oxigeniertes Perfluorcarbon das Membranpotential von hypoxischen Herzmuskelzellen stabilisiert und ihre ATP-Produktion verbessert. Es konnte ebenfalls gezeigt werden, dass der Anstieg der Superoxidproduktion in der frühen Phase nach Gabe von oxPFC und die Reduktion des Superoxides in der späten Phase nach oxPFC die Überlebenswahrscheinlichkeit von hypoxischen Kardiomyozyten deutlich erhöht. Mit dieser Arbeit wurden erstmals die physiologischen Mechanismen geklärt, welche zur Reduktion der Infarktgrösse durch oxigeniertes Perfluorocarbon führen. Die Ergebnisse legen nahe, Perfluorcarbone in der Therapie des Herzinfarkts als adjunvante Medikation zur Steigerung der Überlebensrate von Herzmuskelzellen einzusetzen."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Influence of Oxygenated Perfluorocarbon (oxyPFC) on survival and free radical production of Ischemic Cardiomyocytes","Einfluss von oxygeniertem Perfluorokarbon auf das Überleben und die freie Radikalproduktion von ischämischen Kardiomyozyten"]}]}],"canonical_facts":{"dc:contributor":["Hehrlein, Christoph"],"dc:creator":["Wang, Jin"],"dc:description.abstract":["In the late 1980’s, several animal studies showed that oxyPFC given intravenously or via intracoronary route have the potential to reduce the size of myocardial infarctions. The exact mechanism by which this reduction occurred remained unclear until today. The subject of this experimental work was to elucidate the pathways of interaction of oxyPFC with hypoxic cardiomyocytes. The first mechanism we elucidated in this study was the effect of oxyPFC on mitochondrial respiration in a hypoxic state of the cardiomyocyte, and the second was to find out which role oxygen free radicals, especially superoxide, play after administration of oxyPFC. Several cellular biological methods were used to analyse mitochondrial respiration under the influence of oxyPFC. The results show that myocardial viability and ATP-production is increased after application of small doses of oxyPFC. The results further indicated that superoxide is up-regulated in an early phase after oxyPFC application, and down-regulated 6 hours after oxyPFC application. This experimental study clarified some of the physiological mechanisms by which oxyPFC reduces the infarct size. Our results suggest that oxyPFC may be useful as an adjunct therapy in myocardial infarction to increase survival of cardiomyocytes.","Ende der 80er Jahre zeigten eine Reihe von tierexperimentellen Studien, dass die Ausdehnung eines Myokardinfarkts durch intravenöse oder intrakoronare Zugabe von oxigenierten Perfluorocarbonen (oxPFC) deutlich reduziert werden kann. Der Mechanismus dieser Reduktion der Infarktgrösse durch oxigeniertes Perfluorocarbon ist bis heute unklar geblieben. Es war Gegenstand dieser wissenschaftlichen Arbeit an humanen Herzmuskelzellen zu untersuchen, 1. welche Mechanismen im Sauerstoffstoffwechsel für das Überleben von Herzmuskelzellen in der Phase der Hypoxie bzw. Ischämie eine Rolle spielen und 2. aufzuzeigen, welches Wirkungsspektrum oxigeniertes Perfluorocarbon bei der Reoxigenierung von Herzmuskelzellen und deren Produktion von Sauerstoffradikalen, insbesondere der Superoxidproduktion besitzen. Mit mehreren zellbiologischen Methoden konnte gezeigt werden, dass oxigeniertes Perfluorcarbon das Membranpotential von hypoxischen Herzmuskelzellen stabilisiert und ihre ATP-Produktion verbessert. Es konnte ebenfalls gezeigt werden, dass der Anstieg der Superoxidproduktion in der frühen Phase nach Gabe von oxPFC und die Reduktion des Superoxides in der späten Phase nach oxPFC die Überlebenswahrscheinlichkeit von hypoxischen Kardiomyozyten deutlich erhöht. Mit dieser Arbeit wurden erstmals die physiologischen Mechanismen geklärt, welche zur Reduktion der Infarktgrösse durch oxigeniertes Perfluorocarbon führen. Die Ergebnisse legen nahe, Perfluorcarbone in der Therapie des Herzinfarkts als adjunvante Medikation zur Steigerung der Überlebensrate von Herzmuskelzellen einzusetzen."],"dc:format.medium":["application/pdf"],"dc:subject":["Ischemia","Oxygenation","Free Radical Production"],"dc:title":["Influence of Oxygenated Perfluorocarbon (oxyPFC) on survival and free radical production of Ischemic Cardiomyocytes","Einfluss von oxygeniertem Perfluorokarbon auf das Überleben und die freie Radikalproduktion von ischämischen Kardiomyozyten"],"dc:type":["DoctoralThesis"]},"updated_at":"2026-07-24T02:22:16Z"}