Many important antibiotics have become nearly obsolete due to the rise of antibiotic resistant pathogens. Rhodococcus, an actinomycete related to the prolific antibiotic producing genus Streptomyces, harbors over 30 genes for secondary metabolism that could be involved in antibiotic production. Several antibiotics have already been reported for Rhodococcus, suggesting the genus may be a good source for new inhibitory compounds. Fifty four soil bacteria were isolated using enrichment culture techniques (including 37 Rhodococcus) and screened for antibiotic producers. BTHX2, a species of Bacillus was found to have activity against Micrococcus luteus and Rhodococcus erythropolis. BTHX2 has a 16S rDNA sequence 97% homologous to Bacillus licheniformis, and may be a new strain of B. licheniformis. The inhibitory substance produced by BTHX2 was and found to have a spectrum of activity against a broad Gram-positive bacteria and some fungus, and may have cytolytic activity.
","abstract_html":"<p>Many important antibiotics have become nearly obsolete due to the rise of antibiotic resistant pathogens. <em>Rhodococcus</em>, an actinomycete related to the prolific antibiotic producing genus <em>Streptomyces</em>, harbors over 30 genes for secondary metabolism that could be involved in antibiotic production. Several antibiotics have already been reported for <em>Rhodococcus</em>, suggesting the genus may be a good source for new inhibitory compounds. Fifty four soil bacteria were isolated using enrichment culture techniques (including 37 <em>Rhodococcus</em>) and screened for antibiotic producers. BTHX2, a species of <em>Bacillus</em> was found to have activity against <em>Micrococcus luteus</em> and <em>Rhodococcus erythropolis</em>. BTHX2 has a 16S rDNA sequence 97% homologous to <em>Bacillus licheniformis</em>, and may be a new strain of <em>B. licheniformis</em>. The inhibitory substance produced by BTHX2 was and found to have a spectrum of activity against a broad Gram-positive bacteria and some fungus, and may have cytolytic activity.</p>","abstract_has_math":false,"creators":["Barber, Thomas S"],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - unrestricted","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-12-18T08:00:00Z","date_published":"2010-12-18T08:00:00Z","updated_at":"2026-07-24T02:20:55Z","subjects":["antibiotic","antimicrobial","Bacillus","Rhodococcus","Bacteriology","Life Sciences","Microbiology"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/1777","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Barber, Thomas S"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2010-12-18T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - unrestricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["antibiotic","antimicrobial","Bacillus","Rhodococcus","Bacteriology","Life Sciences","Microbiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/3132/viewcontent/BarberT120810f.pdf","https://dc.etsu.edu/etd/1777"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Many important antibiotics have become nearly obsolete due to the rise of antibiotic resistant pathogens. Rhodococcus, an actinomycete related to the prolific antibiotic producing genus Streptomyces, harbors over 30 genes for secondary metabolism that could be involved in antibiotic production. Several antibiotics have already been reported for Rhodococcus, suggesting the genus may be a good source for new inhibitory compounds. Fifty four soil bacteria were isolated using enrichment culture techniques (including 37 Rhodococcus) and screened for antibiotic producers. BTHX2, a species of Bacillus was found to have activity against Micrococcus luteus and Rhodococcus erythropolis. BTHX2 has a 16S rDNA sequence 97% homologous to Bacillus licheniformis, and may be a new strain of B. licheniformis. The inhibitory substance produced by BTHX2 was and found to have a spectrum of activity against a broad Gram-positive bacteria and some fungus, and may have cytolytic activity.
"]},{"key":"dc:title","label":"Title","values":["Discovery and Characterization of an Antibiotic from the Soil Bacterium Bacillus sp."]}]}],"canonical_facts":{"dc:creator":["Barber, Thomas S"],"dc:date.issued":["2010-12-18T08:00:00Z"],"dc:description.abstract":["Many important antibiotics have become nearly obsolete due to the rise of antibiotic resistant pathogens. Rhodococcus, an actinomycete related to the prolific antibiotic producing genus Streptomyces, harbors over 30 genes for secondary metabolism that could be involved in antibiotic production. Several antibiotics have already been reported for Rhodococcus, suggesting the genus may be a good source for new inhibitory compounds. Fifty four soil bacteria were isolated using enrichment culture techniques (including 37 Rhodococcus) and screened for antibiotic producers. BTHX2, a species of Bacillus was found to have activity against Micrococcus luteus and Rhodococcus erythropolis. BTHX2 has a 16S rDNA sequence 97% homologous to Bacillus licheniformis, and may be a new strain of B. licheniformis. The inhibitory substance produced by BTHX2 was and found to have a spectrum of activity against a broad Gram-positive bacteria and some fungus, and may have cytolytic activity.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/3132/viewcontent/BarberT120810f.pdf","https://dc.etsu.edu/etd/1777"],"dc:rights":["Copyright by the authors."],"dc:subject":["antibiotic","antimicrobial","Bacillus","Rhodococcus","Bacteriology","Life Sciences","Microbiology"],"dc:title":["Discovery and Characterization of an Antibiotic from the Soil Bacterium Bacillus sp."],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis - unrestricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:20:55Z"}