The soil bacterium Rhodococcus has a wide array of secondary metabolic pathways such as production of pigments, siderophores, and antibiotics that makes it an organism of interest for the production of novel natural products. Analysis of the genome sequence of Rhodococcus indicates the presence of 24 non-ribosomal peptide synthases and 7 polyketide synthases possibly involved in production of secondary metabolites. The use of a solid agar extraction method to screen soil isolates of Rhodococcus for compounds with inhibitory activity against other bacteria resulted in the discovery of a promising candidate molecule. The Rhodococcus strain KCHXC3, isolated from eastern Tennessee soil, produces a substance that inhibits the growth of several Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia and Gram-positive bacteria such as Micrococcus luteus and, Staphylococcus aureus. After bulk extraction of this compound with ethyl acetate from agar plates, the material was partially purified through different chromatography processes.
","abstract_html":"<p>The soil bacterium <em>Rhodococcus</em> has a wide array of secondary metabolic pathways such as production of pigments, siderophores, and antibiotics that makes it an organism of interest for the production of novel natural products. Analysis of the genome sequence of <em>Rhodococcus</em> indicates the presence of 24 non-ribosomal peptide synthases and 7 polyketide synthases possibly involved in production of secondary metabolites. The use of a solid agar extraction method to screen soil isolates of <em>Rhodococcus</em> for compounds with inhibitory activity against other bacteria resulted in the discovery of a promising candidate molecule. The <em>Rhodococcus</em> strain KCHXC3, isolated from eastern Tennessee soil, produces a substance that inhibits the growth of several Gram-negative bacteria such as <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, and <em>Klebsiella pneumonia</em> and Gram-positive bacteria such as <em>Micrococcus luteus</em> and, <em>Staphylococcus aureus</em>. After bulk extraction of this compound with ethyl acetate from agar plates, the material was partially purified through different chromatography processes.</p>","abstract_has_math":false,"creators":["Carr, Megan"],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - restricted","degree_discipline":"Biomedical Sciences","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-12-15T08:00:00Z","date_published":"2012-12-15T08:00:00Z","updated_at":"2026-07-24T02:20:42Z","subjects":["Bioactive Compound","Natural Product","Antibiotic","Rhodococcus","Purification","Earth Sciences","Physical Sciences and Mathematics","Soil Science"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/1479","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Carr, Megan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2012-12-15T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - restricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bioactive Compound","Natural Product","Antibiotic","Rhodococcus","Purification","Earth Sciences","Physical Sciences and Mathematics","Soil Science"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/2672/viewcontent/CarrM081512f.pdf","https://dc.etsu.edu/etd/1479"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The soil bacterium Rhodococcus has a wide array of secondary metabolic pathways such as production of pigments, siderophores, and antibiotics that makes it an organism of interest for the production of novel natural products. Analysis of the genome sequence of Rhodococcus indicates the presence of 24 non-ribosomal peptide synthases and 7 polyketide synthases possibly involved in production of secondary metabolites. The use of a solid agar extraction method to screen soil isolates of Rhodococcus for compounds with inhibitory activity against other bacteria resulted in the discovery of a promising candidate molecule. The Rhodococcus strain KCHXC3, isolated from eastern Tennessee soil, produces a substance that inhibits the growth of several Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia and Gram-positive bacteria such as Micrococcus luteus and, Staphylococcus aureus. After bulk extraction of this compound with ethyl acetate from agar plates, the material was partially purified through different chromatography processes.
"]},{"key":"dc:title","label":"Title","values":["Detection and Purification of a Novel Natural Inhibitory Compound from an Isolated Strain of Rhodococcus Using an Agar Extraction Method"]}]}],"canonical_facts":{"dc:creator":["Carr, Megan"],"dc:date.issued":["2012-12-15T08:00:00Z"],"dc:description.abstract":["The soil bacterium Rhodococcus has a wide array of secondary metabolic pathways such as production of pigments, siderophores, and antibiotics that makes it an organism of interest for the production of novel natural products. Analysis of the genome sequence of Rhodococcus indicates the presence of 24 non-ribosomal peptide synthases and 7 polyketide synthases possibly involved in production of secondary metabolites. The use of a solid agar extraction method to screen soil isolates of Rhodococcus for compounds with inhibitory activity against other bacteria resulted in the discovery of a promising candidate molecule. The Rhodococcus strain KCHXC3, isolated from eastern Tennessee soil, produces a substance that inhibits the growth of several Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia and Gram-positive bacteria such as Micrococcus luteus and, Staphylococcus aureus. After bulk extraction of this compound with ethyl acetate from agar plates, the material was partially purified through different chromatography processes.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/2672/viewcontent/CarrM081512f.pdf","https://dc.etsu.edu/etd/1479"],"dc:rights":["Copyright by the authors."],"dc:subject":["Bioactive Compound","Natural Product","Antibiotic","Rhodococcus","Purification","Earth Sciences","Physical Sciences and Mathematics","Soil Science"],"dc:title":["Detection and Purification of a Novel Natural Inhibitory Compound from an Isolated Strain of Rhodococcus Using an Agar Extraction Method"],"thesis:degree_discipline":["Biomedical Sciences"],"thesis:degree_level":["Thesis - restricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:20:42Z"}