Subcellular Localization of Tobacco Salicylic Acid Binding Protein 2 in Plants.

<p>Salicylic Acid Binding Protein 2 (SABP2) is a 29kDa protein present in extremely low amounts in tobacco leaves. SABP2 processes the mobile defense signal, methyl salicylic acid generated in plants resisting microbial infection. The precise localization of SABP2 in plants is not known. SABP2 has not been shown to have any targeting signal peptides. This study was designed to determine localization of SABP2 in tobacco plants. Biochemical and immunological studies using antibodies against SABP2 suggest that it is localized to the chloroplast, associating with chloroplast envelope membranes. Chloroplast import assays confirm that SABP2 is associated with the chloroplast envelope membrane. Solubilization and analysis of chloroplast membrane proteins show that imported SABP2 associates with the chloroplast envelope membrane by weak hydrophobic and/or ionic interactions. Cellular localization and understanding mechanisms of SABP2 import to the chloroplast will be important from a metabolic engineering standpoint to enhance plant natural defense against microbial pathogens.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - restricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2011

Author dc:creator

• Fai, Leonard Yenwong

• Plant defense
• salicylic acid
• systemic acquired resistance
• salicylic acid binding protein 2
• sub-cellular localization
• chloroplast import
• Life Sciences
• Plant Biology
• Plant Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1274

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2465