The biogenic amines serotonin (5HT), dopamine (DA), and octopamine (OA) have been indicated in the regulation of behaviors, including aggression. The flesh fly, Sarcophaga crassipalpis, was used to investigate ontogenetic and circadian changes in amines and aggression. Heads of male flies were analyzed for amine content using high performance liquid chromatography with electrochemical detection (HPLC-ECD) at 3 time points on each of 4 consecutive days in 2 light cycles, 12:12 LD and 15:9 LD. Both DA and OA levels decreased with age. Light-cycle dependent differences were observed for all amines in overall levels and patterns of change throughout the day. A behavioral assay quantified interactive and aggressive behaviors at three time points in the light period for 2 age groups. The daily changes in behavioral profiles differed dependent on age. Correlations from these data can be made between changes in amine levels and time of day, photoperiod, age, and aggressive state.
","abstract_html":"<p>The biogenic amines serotonin (5HT), dopamine (DA), and octopamine (OA) have been indicated in the regulation of behaviors, including aggression. The flesh fly, <em>Sarcophaga crassipalpis</em>, was used to investigate ontogenetic and circadian changes in amines and aggression. Heads of male flies were analyzed for amine content using high performance liquid chromatography with electrochemical detection (HPLC-ECD) at 3 time points on each of 4 consecutive days in 2 light cycles, 12:12 LD and 15:9 LD. Both DA and OA levels decreased with age. Light-cycle dependent differences were observed for all amines in overall levels and patterns of change throughout the day. A behavioral assay quantified interactive and aggressive behaviors at three time points in the light period for 2 age groups. The daily changes in behavioral profiles differed dependent on age. Correlations from these data can be made between changes in amine levels and time of day, photoperiod, age, and aggressive state.</p>","abstract_has_math":false,"creators":["Fregoso, Veronica L."],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - unrestricted","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-12-01T08:00:00Z","date_published":"2012-12-01T08:00:00Z","updated_at":"2026-07-24T02:20:06Z","subjects":["flesh fly","octopamine","dopamine","serotonin","biogenic amines","aggression","Biology","Life Sciences"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/1225","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Fregoso, Veronica L."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-01T07:00:00Z"]},{"key":"dc:date.issued","label":"Date","values":["2012-12-01T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - unrestricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["flesh fly","octopamine","dopamine","serotonin","biogenic amines","aggression","Biology","Life Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/2411/viewcontent/FregosoV120312f.pdf","https://dc.etsu.edu/etd/1225"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The biogenic amines serotonin (5HT), dopamine (DA), and octopamine (OA) have been indicated in the regulation of behaviors, including aggression. The flesh fly, Sarcophaga crassipalpis, was used to investigate ontogenetic and circadian changes in amines and aggression. Heads of male flies were analyzed for amine content using high performance liquid chromatography with electrochemical detection (HPLC-ECD) at 3 time points on each of 4 consecutive days in 2 light cycles, 12:12 LD and 15:9 LD. Both DA and OA levels decreased with age. Light-cycle dependent differences were observed for all amines in overall levels and patterns of change throughout the day. A behavioral assay quantified interactive and aggressive behaviors at three time points in the light period for 2 age groups. The daily changes in behavioral profiles differed dependent on age. Correlations from these data can be made between changes in amine levels and time of day, photoperiod, age, and aggressive state.
"]},{"key":"dc:title","label":"Title","values":["Biogenic Amine Levels Correlate with Time of Day, Age, Light Cycle, and Aggressive State in the Flesh Fly, Sarcophaga crassipalpis"]}]}],"canonical_facts":{"dc:creator":["Fregoso, Veronica L."],"dc:date.available":["2014-07-01T07:00:00Z"],"dc:date.issued":["2012-12-01T08:00:00Z"],"dc:description.abstract":["The biogenic amines serotonin (5HT), dopamine (DA), and octopamine (OA) have been indicated in the regulation of behaviors, including aggression. The flesh fly, Sarcophaga crassipalpis, was used to investigate ontogenetic and circadian changes in amines and aggression. Heads of male flies were analyzed for amine content using high performance liquid chromatography with electrochemical detection (HPLC-ECD) at 3 time points on each of 4 consecutive days in 2 light cycles, 12:12 LD and 15:9 LD. Both DA and OA levels decreased with age. Light-cycle dependent differences were observed for all amines in overall levels and patterns of change throughout the day. A behavioral assay quantified interactive and aggressive behaviors at three time points in the light period for 2 age groups. The daily changes in behavioral profiles differed dependent on age. Correlations from these data can be made between changes in amine levels and time of day, photoperiod, age, and aggressive state.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/2411/viewcontent/FregosoV120312f.pdf","https://dc.etsu.edu/etd/1225"],"dc:rights":["Copyright by the authors."],"dc:subject":["flesh fly","octopamine","dopamine","serotonin","biogenic amines","aggression","Biology","Life Sciences"],"dc:title":["Biogenic Amine Levels Correlate with Time of Day, Age, Light Cycle, and Aggressive State in the Flesh Fly, Sarcophaga crassipalpis"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis - unrestricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:20:06Z"}