Iron is essential to the majority of microorganisms; it is an important cofactor in many cellular processes and enzymes. However in an aerobic environment and at biological pH, iron is primarily found as insoluble oxyhydroxides and is unavailable to microorganisms. Many bacteria have the ability to produce siderophores, low molecular weight compounds that have a high affinity for Fe3+. Siderophores are part of a multi-component system that actively transports the iron-siderophore complex into the cytoplasm. Rhizobia are characterized by their ability to form symbiotic relationships with leguminous plants, where they can fix nitrogen for the host plant and the plant provides the bacteria with nutrients. Under iron-limiting conditions, Rhizobia are known to produce siderophores. Rhizobium leguminosarum IARI 917 produces one dihydroxamate-type siderophore. This siderophore has been purified and chemically characterized. Results indicate that this strain is producing schizokinen, which has not been described in a member of the Rhizobia family.
","abstract_html":"<p>Iron is essential to the majority of microorganisms; it is an important cofactor in many cellular processes and enzymes. However in an aerobic environment and at biological pH, iron is primarily found as insoluble oxyhydroxides and is unavailable to microorganisms. Many bacteria have the ability to produce siderophores, low molecular weight compounds that have a high affinity for Fe<sup>3+</sup>. Siderophores are part of a multi-component system that actively transports the iron-siderophore complex into the cytoplasm. Rhizobia are characterized by their ability to form symbiotic relationships with leguminous plants, where they can fix nitrogen for the host plant and the plant provides the bacteria with nutrients. Under iron-limiting conditions, Rhizobia are known to produce siderophores. <em>Rhizobium leguminosarum</em> IARI 917 produces one dihydroxamate-type siderophore. This siderophore has been purified and chemically characterized. Results indicate that this strain is producing schizokinen, which has not been described in a member of the Rhizobia family.</p>","abstract_has_math":false,"creators":["Storey, Erin P."],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - unrestricted","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2005,"date_issued":"2005-08-16T07:00:00Z","date_published":"2005-08-16T07:00:00Z","updated_at":"2026-07-24T02:19:43Z","subjects":["iron transport","siderophore","hydroxamate","Rhizobium leguminosarum","schizokinen","Biochemistry, Biophysics, and Structural Biology","Life Sciences"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/1050","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Storey, Erin P."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2005-08-16T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - unrestricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["iron transport","siderophore","hydroxamate","Rhizobium leguminosarum","schizokinen","Biochemistry, Biophysics, and Structural Biology","Life Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/2207/viewcontent/StoreyE080405f.pdf","https://dc.etsu.edu/etd/1050"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Iron is essential to the majority of microorganisms; it is an important cofactor in many cellular processes and enzymes. However in an aerobic environment and at biological pH, iron is primarily found as insoluble oxyhydroxides and is unavailable to microorganisms. Many bacteria have the ability to produce siderophores, low molecular weight compounds that have a high affinity for Fe3+. Siderophores are part of a multi-component system that actively transports the iron-siderophore complex into the cytoplasm. Rhizobia are characterized by their ability to form symbiotic relationships with leguminous plants, where they can fix nitrogen for the host plant and the plant provides the bacteria with nutrients. Under iron-limiting conditions, Rhizobia are known to produce siderophores. Rhizobium leguminosarum IARI 917 produces one dihydroxamate-type siderophore. This siderophore has been purified and chemically characterized. Results indicate that this strain is producing schizokinen, which has not been described in a member of the Rhizobia family.
"]},{"key":"dc:title","label":"Title","values":["Isolation, Purification, and Chemical Characterization of the Dihydroxamate-Type Siderophore, \"Schizokinen,\" Produced by Rhizobium leguminosarum IARI 917."]}]}],"canonical_facts":{"dc:creator":["Storey, Erin P."],"dc:date.issued":["2005-08-16T07:00:00Z"],"dc:description.abstract":["Iron is essential to the majority of microorganisms; it is an important cofactor in many cellular processes and enzymes. However in an aerobic environment and at biological pH, iron is primarily found as insoluble oxyhydroxides and is unavailable to microorganisms. Many bacteria have the ability to produce siderophores, low molecular weight compounds that have a high affinity for Fe3+. Siderophores are part of a multi-component system that actively transports the iron-siderophore complex into the cytoplasm. Rhizobia are characterized by their ability to form symbiotic relationships with leguminous plants, where they can fix nitrogen for the host plant and the plant provides the bacteria with nutrients. Under iron-limiting conditions, Rhizobia are known to produce siderophores. Rhizobium leguminosarum IARI 917 produces one dihydroxamate-type siderophore. This siderophore has been purified and chemically characterized. Results indicate that this strain is producing schizokinen, which has not been described in a member of the Rhizobia family.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/2207/viewcontent/StoreyE080405f.pdf","https://dc.etsu.edu/etd/1050"],"dc:rights":["Copyright by the authors."],"dc:subject":["iron transport","siderophore","hydroxamate","Rhizobium leguminosarum","schizokinen","Biochemistry, Biophysics, and Structural Biology","Life Sciences"],"dc:title":["Isolation, Purification, and Chemical Characterization of the Dihydroxamate-Type Siderophore, \"Schizokinen,\" Produced by Rhizobium leguminosarum IARI 917."],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis - unrestricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:19:43Z"}