Caprine Arthirtis Encephalitis Virus (CAEV) and Visna Virus are two viruses of the lentivirus family. They encode three structural genes (gag, pol, and env) and two regulatory genes (rev and tat). The Rev protein regulates Gag, Pol and Env expression by transporting their mRNAs to the cytoplasm by binding to the RRE (Rev Response Element) found on their mRNAs. Previous studies have indicated that Rev may be toxic to transfected cells, overexpression of exogenous RREs or a better binding RRE can inhibit Rev activity and Rev-C (CAEV Rev) can trans-activate RRE-V (Visna Virus RRE). To test these possibilities FACS analysis, RNA binding assays, cotransfections, and SELEX were done. The results indicated that Rev is not acutely toxic to cells, inhibition of Rev activity could not be achieved by making a better binder or through expression of exogenous RREs, and Rev-C can trans-activate RRE-V implicating conservation of Rev/RRE interactions in lentiviruses.
","abstract_html":"<p>Caprine Arthirtis Encephalitis Virus (CAEV) and Visna Virus are two viruses of the lentivirus family. They encode three structural genes (<em>gag</em>, <em>pol</em>, and <em>env</em>) and two regulatory genes (<em>rev</em> and <em>tat</em>). The Rev protein regulates Gag, Pol and Env expression by transporting their mRNAs to the cytoplasm by binding to the RRE (Rev Response Element) found on their mRNAs. Previous studies have indicated that Rev may be toxic to transfected cells, overexpression of exogenous RREs or a better binding RRE can inhibit Rev activity and Rev-C (CAEV Rev) can <em>trans</em>-activate RRE-V (Visna Virus RRE). To test these possibilities FACS analysis, RNA binding assays, cotransfections, and SELEX were done. The results indicated that Rev is not acutely toxic to cells, inhibition of Rev activity could not be achieved by making a better binder or through expression of exogenous RREs, and Rev-C can <em>trans</em>-activate RRE-V implicating conservation of Rev/RRE interactions in lentiviruses.</p>","abstract_has_math":false,"creators":["Graves, Bridget Michele"],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - unrestricted","degree_discipline":"Biomedical Sciences","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2001,"date_issued":"2001-12-01T08:00:00Z","date_published":"2001-12-01T08:00:00Z","updated_at":"2026-07-24T02:18:50Z","subjects":["Rev","Visna Virus","CAEV","RRE","Medical Sciences","Medicine and Health Sciences"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/45","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Graves, Bridget Michele"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["1900-01-01T08:00:00Z"]},{"key":"dc:date.issued","label":"Date","values":["2001-12-01T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - unrestricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Rev","Visna Virus","CAEV","RRE","Medical Sciences","Medicine and Health Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/1095/viewcontent/GravesB.pdf","https://dc.etsu.edu/etd/45"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Caprine Arthirtis Encephalitis Virus (CAEV) and Visna Virus are two viruses of the lentivirus family. They encode three structural genes (gag, pol, and env) and two regulatory genes (rev and tat). The Rev protein regulates Gag, Pol and Env expression by transporting their mRNAs to the cytoplasm by binding to the RRE (Rev Response Element) found on their mRNAs. Previous studies have indicated that Rev may be toxic to transfected cells, overexpression of exogenous RREs or a better binding RRE can inhibit Rev activity and Rev-C (CAEV Rev) can trans-activate RRE-V (Visna Virus RRE). To test these possibilities FACS analysis, RNA binding assays, cotransfections, and SELEX were done. The results indicated that Rev is not acutely toxic to cells, inhibition of Rev activity could not be achieved by making a better binder or through expression of exogenous RREs, and Rev-C can trans-activate RRE-V implicating conservation of Rev/RRE interactions in lentiviruses.
"]},{"key":"dc:title","label":"Title","values":["Molecular Studies involving the Rev Protein of Caprine Arthritis Encephalitis Virus and Visna Virus."]}]}],"canonical_facts":{"dc:creator":["Graves, Bridget Michele"],"dc:date.available":["1900-01-01T08:00:00Z"],"dc:date.issued":["2001-12-01T08:00:00Z"],"dc:description.abstract":["Caprine Arthirtis Encephalitis Virus (CAEV) and Visna Virus are two viruses of the lentivirus family. They encode three structural genes (gag, pol, and env) and two regulatory genes (rev and tat). The Rev protein regulates Gag, Pol and Env expression by transporting their mRNAs to the cytoplasm by binding to the RRE (Rev Response Element) found on their mRNAs. Previous studies have indicated that Rev may be toxic to transfected cells, overexpression of exogenous RREs or a better binding RRE can inhibit Rev activity and Rev-C (CAEV Rev) can trans-activate RRE-V (Visna Virus RRE). To test these possibilities FACS analysis, RNA binding assays, cotransfections, and SELEX were done. The results indicated that Rev is not acutely toxic to cells, inhibition of Rev activity could not be achieved by making a better binder or through expression of exogenous RREs, and Rev-C can trans-activate RRE-V implicating conservation of Rev/RRE interactions in lentiviruses.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/1095/viewcontent/GravesB.pdf","https://dc.etsu.edu/etd/45"],"dc:rights":["Copyright by the authors."],"dc:subject":["Rev","Visna Virus","CAEV","RRE","Medical Sciences","Medicine and Health Sciences"],"dc:title":["Molecular Studies involving the Rev Protein of Caprine Arthritis Encephalitis Virus and Visna Virus."],"thesis:degree_discipline":["Biomedical Sciences"],"thesis:degree_level":["Thesis - unrestricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:18:50Z"}